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    NOVEL PROMOTERS AND GENE EXPRESSION METHOD BY USING THE PROMOTERS
    5.
    发明公开
    NOVEL PROMOTERS AND GENE EXPRESSION METHOD BY USING THE PROMOTERS 审中-公开
    新启动子与基因表达中的方法使用SAME

    公开(公告)号：EP1291432A4

    公开(公告)日：2004-07-14

    申请号：EP01925940

    申请日：2001-04-26

    申请人： BIOLEADERS CORP ,  TAKARA BIO INC

    发明人： SUNG MOON-HEE ,  LEE SEUNG-GOO ,  HONG SEUNG-PYO ,  SEO HWA-JUNG

    IPC分类号： C07K14/32 ,  C12N1/21 ,  C12N15/70 ,  C12N15/75 ,  C12P21/02

    CPC分类号： C07K14/32 ,  C12N15/70 ,  C12N15/75

    摘要： Promoters capable of expressing a gene at a high level without inducing the gene expression; recombinant DNAs; vectors for gene expression; expression vectors; transformed cells; a process for producing a protein which can be conveniently performed at a low cost; and a kit therefor. Namely, an isolated DNA having a base sequence represented by SEQ ID NO:1 or 2 in Sequence Listing or its fragment showing a constitutive promoter activity in Escherichia coli or bacteria belonging to the genus Bacillus ; an isolated DNA hybridizable with the above DNA and showing a constitutive promoter activity in Escherichia coli or bacteria belonging to the genus Bacillus ; a recombinant DNA wherein the above DNA and a foreign gene are provided in such a state as enabling the expression of the foreign gene; a vector for gene expression which contains at least the above DNA; an expression vector containing the above recombinant DNA; a transformed cells carrying the above recombinant DNA or the above expression vector; a process for producing a protein which comprises culturing the above transformed cell and collecting the protein from the culture medium thus obtained; and a kit for producing a protein which contains at least the above DNA or the above vector for gene expression.