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    ASSAY FOR INHIBITORS OF ISPH
    2.
    发明授权
    ASSAY FOR INHIBITORS OF ISPH 有权
    分析法的抑制剂ISPH

    公开(公告)号：EP1585826B1

    公开(公告)日：2006-11-22

    申请号：EP04702324.7

    申请日：2004-01-15

    申请人： Bacher, Adelbert

    发明人： BACHER, Adelbert ,  ADAM, Petra ,  EISENREICH, Wolfgang ,  GRÄWERT, Tobias ,  HECHT, Stefan ,  ROHDICH, Felix ,  ZEPECK, Ferdinand

    IPC分类号： C12Q1/26

    CPC分类号： C12Q1/26

    摘要： Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P) ; c) repeating step (a) in the presence of the sample to be tested; d) repeating step (b) with the reaction mixture defined in step (c); e) comparing the results of steps (b) and (d).

    THE NON-MEVALONATE ISOPRENOID PATHWAY
    4.
    发明公开
    THE NON-MEVALONATE ISOPRENOID PATHWAY 有权
    非偏向性异丙肾上腺素途径

    公开(公告)号：EP1287145A2

    公开(公告)日：2003-03-05

    申请号：EP01940547.1

    申请日：2001-06-01

    申请人： Bacher, Adelbert ,  Zenk, Meinhart H.

    发明人： BACHER, Adelbert ,  ZENK, Meinhart, H. ,  ADAM, Petra ,  EISENREICH, Wolfgang ,  FELLERMEIER, Monika ,  HECHT, Stefan ,  ROHDICH, Felix ,  SCHUHR, Christoph, A. ,  WUNGSINTAWEEKUL, Juraithip

    IPC分类号： C12N15/52 ,  C12N15/82 ,  C12N9/00 ,  C12P9/00 ,  A01H5/10

    CPC分类号： C12N9/00 ,  C12N15/52 ,  C12N15/8274 ,  C12P9/00

    摘要： The present invention is directed to enzymes and intermediates of the non-mevalonate isoprenoid pathway downstream of 2C-methyl-D-erythritol 2,4-cyclopyrophosphate and upstream of isopentenyl pyrophosphate or dimethylallyl pyrophosphate. These are used as a basis for a screening method for inhibitors of these enzymes, and a method for identifying inhibitor-resistant variants thereof. Further disclosures refer to DNA coding for said enzymes and for inhibitor-resistant variants thereof, vectors containing said DNA, cells containing said vector, and plant seeds comprising cells containing said vector. This invention is useful for the inhibition of the biosynthesis of isoprenoids in plants, bacteria and protozoa, for confering herbicide-resistance to plants, as well as for weed control in agriculture using a crop containing a herbicide-resistant gene and an effective amount of a suitable herbicide.

    摘要翻译： 本发明涉及2C甲基-D-赤藓糖醇2,4-环焦磷酸酯下游和异戊烯基焦磷酸或二甲基烯丙基焦磷酸上游的非甲羟戊酸类异戊二烯途径的酶和中间体。 这些用作这些酶抑制剂筛选方法的基础，以及鉴定其抑制剂抗性变体的方法。 其它公开内容涉及编码所述酶及其抑制剂抗性变体的DNA，含有所述DNA的载体，含有所述载体的细胞和含有含所述载体的细胞的植物种子。 本发明可用于抑制植物，细菌和原生动物中类异戊二烯的生物合成，赋予植物除草剂抗性，以及在农业中用含有除草剂抗性基因和有效量的 合适的除草剂。

    SCREENING FOR INHIBITORS OF LUMAZINE SYNTHASE
    7.
    发明公开
    SCREENING FOR INHIBITORS OF LUMAZINE SYNTHASE 审中-公开
    筛查抑制剂lumazine

    公开(公告)号：EP1208221A2

    公开(公告)日：2002-05-29

    申请号：EP00960438.0

    申请日：2000-08-10

    申请人： Bacher, Adelbert

    发明人： BACHER, Adelbert ,  MÖRTL, Simone

    IPC分类号： C12Q1/48 ,  C12N9/10

    CPC分类号： C12N9/1085 ,  C12N15/8274 ,  C12Q1/48

    摘要： A method is described for screening for the presence or absence of inhibition of 6,7-dimethyl-8-ribityllumazine synthase activity comprising the following steps : (a) preparing a first aqueous mixture containing a protein having a plant-type 6,7-dimethyl-8-ribityllumazine synthase sequence, 5-amino-6-ribitylamino-2,4(1H,3H)pyrimidine-dione and 3,4-dihydroxy-2-butanone 4-phosphate; (b) reacting said first mixture during a predetermined period of time at a predetermined temperature and subsequently detecting the level of 6,7-dimethyl-8-ribityllumazine; (c) preparing a second aqueous mixture by including in said first mixture a predetermined amount of a chemical test sample; (d) reacting said second mixture during the predetermined period of time at the predetermined temperature and subsequently detecting the level of 6,7-dimethyl-8-ribityllumazine; (e) determining the presence of inhibition of 6,7-dimethyl-8-ribityllumazine synthase by observation of whether the level detected in step (d) is lower than the level detected in step (b).