公开(公告)号：EP1171632B1

公开(公告)日：2010-08-18

申请号：EP00914690.3

申请日：2000-02-24

申请人： GILEAD SCIENCES, INC.

发明人： JAYASENA, Sumedha ,  GOLD, Larry

IPC分类号： C12Q1/68

CPC分类号： C07H21/00 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/321 ,  C12Q1/6848 ,  G01N2333/96433 ,  C12Q2549/101 ,  C12Q2525/205 ,  C12N2310/3533

摘要： This invention discloses high-affinity oligonucleotide ligands to the thermostable Taq polymerase, Tth polymerase and TZ05 polymerase. Specifically, this invention discloses DNA ligands having the ability to bind to the Taq, Tth and TZ05 polymerases and the methods for obtaining such ligands. The ligands are capable of inhibiting polymerases at any predetermined temperature.

公开(公告)号：EP1198589B1

公开(公告)日：2008-08-20

申请号：EP00911657.5

申请日：2000-01-28

申请人： GILEAD SCIENCES, INC.

发明人： HICKE, Brian ,  WARREN, Stephen ,  PARMA, David ,  GOLD, Larry

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/115 ,  A61K38/00 ,  A61K47/54 ,  A61K47/547 ,  A61K47/549 ,  A61K2123/00 ,  B82Y5/00 ,  C07H19/06 ,  C07H19/10 ,  C07H21/00 ,  C07K14/001 ,  C12N9/1276 ,  C12N15/1048 ,  C12N2310/13 ,  C12N2310/317 ,  C12N2310/3183 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3517 ,  C12N2310/53 ,  C12Q1/37 ,  C12Q1/6883 ,  C12Q2541/101 ,  C12Q2600/158 ,  C40B40/00 ,  F02B2075/027 ,  G01N33/531 ,  G01N33/532 ,  G01N33/535 ,  G01N33/56988 ,  G01N33/68 ,  G01N33/76 ,  G01N2333/16 ,  G01N2333/163 ,  G01N2333/503 ,  G01N2333/575 ,  G01N2333/62 ,  G01N2333/8125 ,  G01N2333/96433 ,  G01N2333/96455 ,  G01N2333/966 ,  G01N2333/9726 ,  G01N2333/974 ,  G01N2333/976 ,  C12N2310/3521

摘要： Methods are described for the identification and preparation of nucleic acid ligands to tenascin-C. Included in the invention are specific RNA ligands to tenascin-C identified by the SELEX method. Further included in the invention are methods for detecting the presence of a disease condition in a biological tissue in which tenascin-C is expressed.

公开(公告)号：EP1204672A1

公开(公告)日：2002-05-15

申请号：EP00950789.8

申请日：2000-07-26

申请人： GILEAD SCIENCES, INC.

发明人： JANJIC, Nebojsa ,  GOLD, Larry

IPC分类号： C07H21/02 ,  C07H21/04 ,  G01N33/566

CPC分类号： C12N15/1138 ,  A61K38/00 ,  C07H21/00 ,  C12N2310/322 ,  G01N33/6863 ,  G01N2500/00

摘要： Methods are described for the identification and preparation of high-affinity nucleic acid ligands to a VEGF receptor. Included in the invention are specific RNA ligands to a VEGF receptor identified by the SELEX method. Also included are RNA ligands that inhibit the interaction of a VEGF receptor with VEGF.

公开(公告)号：EP0957929B1

公开(公告)日：2006-02-22

申请号：EP97912811.3

申请日：1997-10-17

申请人： GILEAD SCIENCES, INC.

发明人： JANJIC, Nebojsa ,  GOLD, Larry ,  SCHMIDT, Paul, G. ,  VARGEESE, Chandra ,  WILLIS, Michael

IPC分类号： A61K31/70 ,  A61K47/48 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/115 ,  A61K9/0019 ,  A61K9/0048 ,  A61K9/127 ,  A61K38/00 ,  A61K47/544 ,  C07H21/00 ,  C07K14/52 ,  C12N15/88 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3515 ,  C12N2310/3521

摘要： This invention discloses a method for preparing a complex comprised of a VEGF Nucleic Acid Ligand and a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound by identifying a VEGF Nucleic Acid Ligand by SELEX methodology and associating the VEGF Nucleic Acid Ligand with a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound. The invention further discloses Complexes comprising one or more VEGF Nucleic Acid Ligands in association with a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound. The invention further includes a Lipid construct comprising a VEGF Nucleic Acid Ligand or Complex and methods for making the same.

公开(公告)号：EP0823914B1

公开(公告)日：2004-08-25

申请号：EP96913880.9

申请日：1996-05-01

申请人： Gilead Sciences, Inc.

发明人： JENSEN, Kirk, B. ,  CHEN, Hang ,  MORRIS, Kevin, N. ,  STEPHENS, Andrew ,  GOLD, Larry

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68 ,  C12N5/06 ,  G01N33/555 ,  C12N15/10

CPC分类号： C12N15/1048 ,  A61K47/547 ,  A61K47/549 ,  C12Q1/6811 ,  G01N2333/96433

摘要： This invention discloses high-affinity oligonucleotide ligands to complex tissue targets, specifically nucleic acid ligands having the ability to bind to complex tissue targets, and the methods for obtaining such ligands. Tissue targets comprise cells, subcellular components, aggregates or cells, collections of cells, and higher ordered structures. Specifically, nucleic acid ligands to red blood cells ghosts, glioblastomas, and lymphomas are described.

公开(公告)号：EP0888374B1

公开(公告)日：2010-05-19

申请号：EP97905737.9

申请日：1997-01-30

申请人： GILEAD SCIENCES, INC.

发明人： BIESECKER, Gregory ,  GOLD, Larry

IPC分类号： C12N15/11 ,  A61K31/712 ,  C12Q1/68 ,  C07H21/04 ,  C07H21/02 ,  A61K31/7125 ,  A61K31/713

CPC分类号： C12N15/115 ,  A61K38/00 ,  A61K45/06 ,  C12N2310/151 ,  C12N2310/315

摘要： Methods are described for the identification and preparation of high affinity nucleic acid ligands to Complement System Proteins. Methods are described for the identification and preparation of high affinity nucleic acid ligands to Complement System Protein, C1q. Included in the invention are specific RNA ligands to C1q identified by the SELEX method.

公开(公告)号：EP0828849B1

公开(公告)日：2006-10-25

申请号：EP96920573.1

申请日：1996-05-30

申请人： GILEAD SCIENCES, INC.

发明人： TOOTHMAN, Penelope, J. ,  GOLD, Larry ,  JANJIC, Nebojsa ,  RINGQUIST, Steven ,  PAGRATIS, Nikos

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/115 ,  B82Y5/00 ,  C07K14/475 ,  C07K14/49 ,  C07K14/495 ,  C07K14/50 ,  C12N15/111 ,  C12N2320/13 ,  G01N2333/96433

摘要： Methods are described for the identification and preparation of high-affinity nucleic acid ligands to TGFβ, PDGF and hKGF. Included in the invention are specific RNA and ssDNA ligands to TGFβ1 and PDGF identified by the SELEX method. Also included in the invention are specific RNA ligands to hKGF identified by the SELEX method. Further included are RNA ligands that inhibit the interaction of TGFβ1 and hKGF with their receptors and DNA ligands that inhibit the interaction of PDGF with its receptor.

公开(公告)号：EP1206575A1

公开(公告)日：2002-05-22

申请号：EP00947549.2

申请日：2000-07-19

申请人： GILEAD SCIENCES, INC.

发明人： SMITH, Jonathan, Drew ,  GOLD, Larry

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6811 ,  C12Q2537/155 ,  C12Q2531/113 ,  C12Q2521/501

摘要： Methods are provided for the production of nucleic acid ligands against target molecules using a procedure known as Transcription-free Systematic Evolution of Ligands by EXponential enrichment (Transcription-free SELEX). The transcription-free SELEX method assembles nucleic acid ligands from fragments of synthetic nucleic acids by annealing those fragments to a complementary template, and then ligating the fragments together.

公开(公告)号：EP1203008A1

公开(公告)日：2002-05-08

申请号：EP00953724.2

申请日：2000-07-28

申请人： Gilead Sciences Inc.

发明人： KORMAN, Alan, J. ,  GOLD, Larry

IPC分类号： C07H21/04 ,  C07H21/02 ,  A61K48/00

CPC分类号： C12N15/1138 ,  A61K48/00

摘要： Methods are provided for generating nucleic acid ligands of CD40ligand. The methods of the invention use the SELEX method for the isolation of nucleic acid ligands. The invention also includes nucleic acid ligands to CD40ligand, and methods and compositions for the treatment and diagnosis of disease using the nucleic acid ligands.

公开(公告)号：EP1203007A1

公开(公告)日：2002-05-08

申请号：EP00948923.8

申请日：2000-07-24

申请人： GILEAD SCIENCES, INC.

发明人： RUCKMAN, Judy ,  GOLD, Larry ,  STEPHENS, Andrew ,  JANJIC, Nebojsa ,  RABIN, Ross ,  LOCHRIE, Michael

IPC分类号： C07H21/04 ,  C07H21/02 ,  A61K48/00 ,  C12Q1/68 ,  C12N15/85 ,  C12N15/86

CPC分类号： C07H21/00 ,  A61K38/00 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/322 ,  G01N33/5308 ,  G01N33/74 ,  G01N2333/4753 ,  C12N2310/3533 ,  C12N2310/321 ,  C12N2310/3521

摘要： The invention provides nucleic acid ligands to hepatocyte growth factor/scatter factor (HGF) and its receptor c-met. The invention also provides nucleic acid ligands to integrins. The nucleic acid ligands of the instant invention are isolated using the SELEX method. SELEX is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The nucleic acid ligands of the invention are useful as diagnostic and therapeutic agents.