公开(公告)号：EP1307548A2

公开(公告)日：2003-05-07

申请号：EP01957149.6

申请日：2001-07-13

申请人： Maxygen, Inc.

发明人： GIVER, Lorraine, J. ,  MINSHULL, Jeremy ,  VOGEL, Kurt

IPC分类号： C12N15/00 ,  C12N9/00

CPC分类号： C12N15/1089 ,  A61K38/00 ,  C12N9/20

摘要： New lipase enzymes (both nucleic acids and polypeptides) are provided. Compositions which include these polypeptides, proteins, nucleic acids, recombinant cells, as well as methods involving the enzymes, antibodies to the enzymes, and methods of using the enzymes are also provided.

公开(公告)号：EP1276861A2

公开(公告)日：2003-01-22

申请号：EP01962421.2

申请日：2001-03-23

申请人： Maxygen, Inc.

发明人： STEMMER, Willem, P., C. ,  MINSHULL, Jeremy ,  KEENAN, Robert, J.

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12Q1/68

CPC分类号： C12N15/1079 ,  C07K2319/00 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/113 ,  C12N15/66 ,  C12N2310/111

摘要： Methods for identifying and controlling the genetic and metabolic pathways underlying complex phenotypes are provided. Conjoint polynucleotide segments that contribute to or disrupt elements of a multigenic phenotype are produced and expressed in cells of interest. Conjoint polynucleotide segments are recombined and/or mutated to give rise to libraries of recombinant concatamers which are expressed in cells of interest. Libraries of conjoint polynucleotide segments and recombinant concatamers are expressed episomally or integrated into the DNA of organelles or chromosomes. Cells are screened or selected to identify members of the population of cells exhibiting a desired phenotype. Libraries and vectors comprising conjoint polynucleotide segments and recombinant concatamers, as well as cells expressing such libraries and vectors or their components are provided. Kits containing conjoint polynucleotide segments, recombinant concatamers, vectors including such polynucleotides, and cells including such polynucleotides and vectors are provided.

公开(公告)号：EP1108058A1

公开(公告)日：2001-06-20

申请号：EP99935574.6

申请日：1999-07-15

申请人： Maxygen, Inc.

发明人： DEL CARDAYRE, Stephen ,  TOBIN, Matthew ,  STEMMER, Willem, P., C. ,  NESS, Jon, E. ,  MINSHULL, Jeremy ,  PATTEN, Phillip, A. ,  SUBRAMANIAN, Venkiteswatan ,  CASTLE, Linda, A. ,  KREBBER, Claus, M. ,  BASS, Steve ,  ZHANG, Ying-Xin ,  COX, Tony ,  HUISMAN, Gjalt ,  YUAN, Ling ,  AFFHOLTER, Joseph, A.

IPC分类号： C12Q1/68 ,  C12N15/00

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：EP1287123B1

公开(公告)日：2011-03-02

申请号：EP01923084.6

申请日：2001-04-02

申请人： Maxygen, Inc. ,  Novozymes A/S

发明人： NESS, Jon, E. ,  WELCH, Mark ,  GIVER, Lorraine, J. ,  CHERRY, Joel, R. ,  BORCHERT, Torben, V. ,  STEMMER, Willem, P., C. ,  MINSHULL, Jeremy

IPC分类号： C12N9/54 ,  C12N15/57 ,  C07K16/40 ,  G06F17/50

CPC分类号： C12N9/6408 ,  C12N9/54

摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.

公开(公告)号：EP1399566A2

公开(公告)日：2004-03-24

申请号：EP01992782.1

申请日：2001-10-29

申请人： Maxygen, Inc. ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： CASTLE, Linda, A. ,  SIEHL, Dan ,  GIVER, Lorraine, J. ,  MINSHULL, Jeremy ,  IVY, Christina ,  CHEN, Yong, Hong ,  DUCK, Nicholas, B. ,  MCCUTCHEN, Billy, F. ,  KEMBLE, Roger ,  PATTEN, Phillip, A.

IPC分类号： C12N15/54 ,  C12N15/82 ,  C12N9/10 ,  A01H5/00

CPC分类号： C12N9/1029 ,  C12N15/8209 ,  C12N15/8275

摘要： Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

摘要翻译： 本文提供新的蛋白质，包括能够催化草甘膦和其他结构相关蛋白质的乙酰化的蛋白质。 还提供了能够编码这些蛋白质的新型多核苷酸，包含这些新蛋白质和/或多核苷酸中的一种或多种的组合物，包含这些新化合物的重组细胞和转基因植物，涉及新化合物的多样化方法，以及使用该化合物的方法。 本文提供的一些新颖的方法和化合物可以用于产生对草甘膦有抗性的生物体，例如植物。

公开(公告)号：EP1373889A2

公开(公告)日：2004-01-02

申请号：EP01957383.1

申请日：2001-07-31

申请人： Maxygen, Inc.

发明人： MINSHULL, Jeremy ,  DAVIS, S., Christopher ,  WELCH, Mark ,  RAILLARD, Sun, Ai ,  VOGEL, Kurt ,  KREBBER, Claus

IPC分类号： G01N33/50

CPC分类号： C12N9/1252 ,  C12N15/1055 ,  C12P19/34 ,  C12Q1/6888

摘要： Methods for sensing test stimuli using arrays of biopolymers are provided. Libraries of biopolymers, such nucleic acid variants, and expression products encoded by nucleic acid variants are provided. Reusable library arrays, and methods for their use are provided.

公开(公告)号：EP1276900A2

公开(公告)日：2003-01-22

申请号：EP01902036.1

申请日：2001-01-10

申请人： Maxygen, Inc.

发明人： BASS, Steven, H. ,  DAVIS, S., Christopher ,  PATTEN, Phillip, A. ,  TOBIN, Matthew ,  MINSHULL, Jeremy ,  WELCH, Mark ,  GUSTAFSSON, Claes ,  CARR, Brian ,  JENNE, Stephane ,  RAILLARD, Sun, Ai ,  CRAMERI, Andreas ,  STEMMER, Willem, P. C. ,  EMIG, Robin ,  LONGCHAMP, Pascal ,  GOLDMAN, Stanley ,  GIVER, Lorraine, J. ,  AFFHOLTER, Joseph A.

IPC分类号： C12Q1/68

CPC分类号： C12N15/1031 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/007 ,  B01J2219/00722 ,  B82Y30/00 ,  C12N15/1027 ,  C40B60/04 ,  G06F19/18 ,  Y02A90/22

摘要： Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.

公开(公告)号：EP1007732A1

公开(公告)日：2000-06-14

申请号：EP98902586.1

申请日：1998-01-16

申请人： Maxygen, Inc.

发明人： DELCARDAYRE, Stephen, B. ,  TOBIN, Mathew, B. ,  STEMMER, Willem, P., C. ,  NESS, Jon, E. ,  MINSHULL, Jeremy ,  PATTEN, Phillip

IPC分类号： C12Q1/68 ,  C12N15/00

CPC分类号： A01H1/00 ,  A01K2217/05 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12Q1/6811

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

摘要翻译： 本发明提供了使用重组和选择/筛选的迭代循环来进行整个细胞和生物体以获得期望特性的方法。 这种性质的实例包括增强的重组性，基因组拷贝数和蛋白质和次级代谢物的表达和/或分泌能力。

公开(公告)号：EP1072010B1

公开(公告)日：2010-04-21

申请号：EP00909923.5

申请日：2000-01-18

申请人： Maxygen, Inc.

发明人： CRAMERI, Andreas ,  STEMMER, Willem, P., C. ,  MINSHULL, Jeremy ,  BASS, Steven, H. ,  WELCH, Mark ,  NESS, Jon, E. ,  GUSTAFSSON, Claes ,  PATTEN, Phillip, A.

IPC分类号： G06F19/00

CPC分类号： C12N15/11 ,  B01J2219/00689 ,  C12N15/1031

摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

公开(公告)号：EP1272967A2

公开(公告)日：2003-01-08

申请号：EP01922889.9

申请日：2001-03-30

申请人： Maxygen, Inc.

发明人： GUSTAFSSON, Claes ,  MINSHULL, Jeremy ,  SELIFONOV, Sergey A. ,  MUNDORFF, Emily ,  EMIG, Robin ,  GOVINADARAJAN, Sridar ,  STEMMER, Willem, P., C. ,  GIVER, Lorraine, J. ,  TOBIN, Matthew ,  DEL CARDAYRE, Stephen ,  PATTEN, Phillip, A.

IPC分类号： G06F19/00

CPC分类号： G06F19/22

摘要： 'In silico' nucleic acid and protein recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided, including design and selection of cross-over events in silico and corresponding nucleic acid synthesis.

摘要翻译： '计算机模拟'核酸和蛋白质重组方法，提供了利用遗传算子和通过计算机改组方法制作的文库的相关整合系统，包括设计和选择计算机上交叉事件和相应的核酸合成。