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公开(公告)号：EP1163260B1
公开(公告)日：2009-05-20
申请号：EP00914217.5
申请日：2000-03-24
发明人： PENTTILÄ, Merja , NAKARI-SETÄLÄ, Tiina , FAGERSTRÖM, Richard , SELBER, Klaus , KULA, Maria-Regina , LINDER, Markus , TJERNELD, Folke
CPC分类号： C07K7/06 , C07K1/14 , C07K7/08 , C07K14/37 , C07K2319/00 , C07K2319/02
摘要： The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.
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2.发明公开
公开(公告)号：EP1204738A1
公开(公告)日：2002-05-15
申请号：EP00954690.4
申请日：2000-08-21
CPC分类号： C07K14/37 , C07K2319/00 , C12N1/14
摘要： This invention relates to a method for decreasing the foam formation during cultivation of a microorganism and to a method for producing an enhanced amount of a product of interest. The method comprises that the microorganism is modified in such a way that the microorganism does not produce an essential amount of at least one of the proteins, polypeptides or peptides associated with foam formation during cultivation of the unmodified microorganism. In particular the method comprises that the microorganism is modified not to produce an essential amount of amphipathic or hydrophobic proteins, polypeptides or peptides.
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公开(公告)号：EP1417235A1
公开(公告)日：2004-05-12
申请号：EP02751227.6
申请日：2002-07-12
CPC分类号： C07K17/00 , C07K14/37 , C07K2319/00
摘要： The present invention relates to immobilization of fusion proteins to surfaces. According to this invention a fusion polypeptide comprises an adhesion polypeptide fused to a preselected polypeptide. Specifically the method takes advantage of the spontaneous immobilization properties of the adhesion polypeptide part of the fusion protein. In one embodiment, the adhesion polypeptide is a fungal hydrophobin.
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公开(公告)号：EP1409641A2
公开(公告)日：2004-04-21
申请号：EP02700294.8
申请日：2002-02-15
CPC分类号： C12N9/0006 , C12Y101/0101 , C12Y101/01012 , Y02E50/17
摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.
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公开(公告)号：EP0981600B1
公开(公告)日：2006-12-13
申请号：EP99907641.7
申请日：1999-03-11
发明人： ARISTIDOU, Aristos , LONDESBOROUGH, John , PENTTILÄ, Merja , RICHARD, Peter , RUOHONEN, Laura , SÖDERLUND, Hans , TELEMAN, Anita , TOIVARI, Mervi
CPC分类号： C12N9/0016 , C12N9/0006 , C12N9/1025 , C12N9/1205 , C12P7/06 , C12P7/18 , C12P7/625 , C12P13/04 , C12P13/08 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to genetic engineering of production microorganisms used in biotechnology to improve their properties so that they produce useful products more efficiently. The microorganisms express at least one enzyme that causes the functional coupling of the oxidation and reduction of substrates by two pyridine nucleotide-linked dehydrogenase reactions with different specificities for the NAD/NADH and NADP/NADPH coenzyme couples and so facilitates the transfer of electrons between the two coenzyme couples through the said substrates. In particular the invention relates to increasing the yields of products such as ethanol or amino acids from carbon and nitrogen sources such as biomass comprising hexoes, pentoses or their polymers.
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7.发明授权
公开(公告)号：EP0665890B1
公开(公告)日：2003-11-19
申请号：EP93921939.0
申请日：1993-10-06
发明人： KERÄNEN, Sirkka , AALTO, Markku , OUTOLA, Mika , RONNE, Hans , PENTTILÄ, Merja
CPC分类号： C12N9/2417 , C12N15/67 , C12N15/80 , C12N15/81
摘要： The invention relates to recombinant DNA technology. Specifically this invention relates to new recombinant eukaryotic cells transformed with SSO genes. Eukaryotic cells transformed with several copies of SSO genes, or overexpressing the Sso protein by some other means, have an increased capacity to produce secreted foreign or endogenous proteins. Further, the said new recombinant cells, when transformed with genes expressing suitable hydrolytic enzymes can utilize appropriate macromolecular compounds more efficiently, which results in increased cell mass production and/or more versatile utilization of the compounds in relevant biotechnical applications.
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公开(公告)号：EP1885848A1
公开(公告)日：2008-02-13
申请号：EP06743559.4
申请日：2006-05-29
摘要： A DNA molecule comprising a fungal gene encoding an enzyme protein capable of converting L-galactonic acid into L-threo-3-deoxy-hexulosonic acid has been cloned and heterologously expressed. The enzyme is involved in the metabolic conversion of sugar acids, which are present in biological waste material such as sugar beet pulp and other pectin comprising material. A microorganism genetically modified to effectively express said enzyme may be used in fermenting biomaterial to desired end products such as ethanol. Alternatively, microorganisms in which the gene has been inactivated may be used to produce L- galactonic acid, which accumulates when the expression of the gene is prevented.
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公开(公告)号：EP1751296A1
公开(公告)日：2007-02-14
申请号：EP05748648.2
申请日：2005-06-06
IPC分类号： C12P7/10
摘要： A process for producing ethanol from a fibrous lignocellulosic raw material. After pre-treatment of the raw material, the fibrous fraction is first hydrolysed at high consistency and then the modified material is subjected simultaneously to continued hydrolysis with a cellulase and to ethanol fermentation in a fermentation mixture. Fermentation is continued to convert an essential portion of the available cellulose into ethanol, then a liquid fraction containing solubilized hemicelluloses is added to the fermentation mixture and fermentation continued. By means of the invention, high fermentation rates, high ethanol concentrations and low ethanol production costs can be attained.
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10.发明授权A METHOD FOR DECREASING THE FOAM FORMATION DURING CULTIVATION OF A MICROORGANISM 有权VERFAHREN ZUR VERMINDERUNG DER SCHAUMBILDUNGWÄHRENDDER KULTIVIERUNG EINES MIKROORGANISMUS
公开(公告)号：EP1204738B1
公开(公告)日：2006-01-25
申请号：EP00954690.4
申请日：2000-08-21
CPC分类号： C07K14/37 , C07K2319/00 , C12N1/14
摘要： This invention relates to a method for decreasing the foam formation during cultivation of a microorganism and to a method for producing an enhanced amount of a product of interest. The method comprises that the microorganism is modified in such a way that the microorganism does not produce an essential amount of at least one of the proteins, polypeptides or peptides associated with foam formation during cultivation of the unmodified microorganism. In particular the method comprises that the microorganism is modified not to produce an essential amount of amphipathic or hydrophobic proteins, polypeptides or peptides.
摘要翻译： 本发明涉及一种降低微生物培养过程中泡沫形成的方法及其生产方法。 该方法包括微生物的修饰，使得微生物在培养未修饰的微生物期间不产生必需量的与泡沫形成相关的至少一种蛋白质，多肽或肽。 特别地，该方法包括修饰微生物不产生必需量的两亲或疏水性蛋白质，多肽或肽。
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