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公开(公告)号：EP0260762A3
公开(公告)日：1988-11-02
申请号：EP87201761
申请日：1987-09-15
申请人： GIST-BROCADES N.V.
发明人： Martin, Juan Francisco , Alvarez, Emilio , Barredo, Jose Luis , Cantoral, Jesus Manuel , Garcia, Bruno Diez
摘要： Novel and efficient methods for preparing Penicillium transformants are provided employing DNA constructs having a structural gene complementing an auxotrophic host and, preferably, the ans gene. Conditions are provided for frequency of transformation. I
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2.发明公开Heterologous polypeptide expressed in filamentous fungi, processes for their preparation, and vectors for their preparation 失效FILAMENTOUS FUNGI中表达的异物多肽，其制备方法及其制备载体
公开(公告)号：EP0215594A3
公开(公告)日：1988-08-31
申请号：EP86306624
申请日：1986-08-27
申请人： GENENCOR, INC.
IPC分类号： C12N15/00 , C12N01/14 , C12P21/02 , C12R01/66 , C12R01/665 , C12R01/785 , C12R01/885
CPC分类号： C12N9/2428 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/61 , C12N9/58 , C12N9/6481 , C12N15/80
摘要： Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3′ terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.
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公开(公告)号：EP0215539A3
公开(公告)日：1988-07-20
申请号：EP86302467
申请日：1986-04-03
申请人： GLAXO GROUP LIMITED
摘要： he present invention provides plasmids suitable as vector systems for filamentous fungi, especially antibiotic-producing filamentous fungi such as antibiotic-producing strains of Acremonium chrysogenum or Penicillium chrysogenum, comprising an autonomous replication sequence which is effective in the intended host, an activator sequence derived from, or copied from, DNA of the intended host and/or one or more other fungi of the same species, and a fungal selectable protein-coding element, transcription of which is directed by the activator sequence.
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4.发明公开Process for producing optically active aryloxypropionic acids and derivatives thereof 失效用于生产光学活性芳族聚氧乙烯酸及其衍生物的方法
公开(公告)号：EP0133033A3
公开(公告)日：1987-04-15
申请号：EP84305113
申请日：1984-07-27
IPC分类号： C12P07/40 , C12P13/00 , C12N01/14 , C07D213/64 , C07D241/44 , C12R01/01 , C12R01/06 , C12R01/07 , C12R01/225 , C12R01/37 , C12R01/465 , C12R01/80
CPC分类号： C07D213/643 , C07D241/44 , C07D277/68 , C12P7/40 , C12P13/001 , C12P17/12 , C12P17/16 , C12P17/18 , C12P41/00 , Y02P20/55
摘要： a-Aryloxypropionic acids and derivatives of formula I below are stereospecifically inverted by contact with a microorganism having a stereospecific inverting enzyme system or with that enzyme system at least partially extracted from the microorganism. This process enables the [S] enantiomer of the compound of formula I: Arthrobacter simplex (NCIB 8929); Bacillus thuringensis (NCIB 11992); Leuconostoc dextranicum (NCIB 2706); Lactobacillus plantarum (NCIB 6105); Proteus vulgaris (NCIB 67) Streptomyces sp. (NCIB 10105); Streptomyces venezuelae (ATCC 15439) and Penicillium sp. (IMI 287163).
wherein G is OR' or
R' is hydrogen or a protecting group and R 2 is hydrogen or methyl, U and V each independently represent hydrogen or halogen, and R is a carboxyl group, or an enzymic equivalent thereof to be converted to the corresponding [R) enantiomer which is a useful intermediate for making the active [R] enantiomer of many herbicides. Specific microorganisms which effect the inversion are:-
公开(公告)号：EP0104918A3
公开(公告)日：1985-06-05
申请号：EP83305710
申请日：1983-09-26
CPC分类号： A01G18/00
摘要： s A process for preparing a substrate suitable for cell cultivation thereon, comprises subjecting cotton to mechanical attrition in the presence of water, and shredding the hydrated cotton. The shredded cotton can be mixed with nutrient, sterilised and used as a mushroom spawn growth or cultivation substrate.
摘要翻译： 用于制造蘑菇产卵的方法包括在水中溶胀谷物，对膨胀的颗粒进行灭菌，例如， 与碳酸钙混合，用培养物接种无菌谷物，例如， 以匀浆的形式，并孵育接种的谷物。 使用CaCO3可防止颗粒聚集。
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公开(公告)号：EP0277736A3
公开(公告)日：1988-08-31
申请号：EP88300545
申请日：1988-01-22
IPC分类号： A01N63/04 , C12N01/14 , C12R01/645
摘要： A method of controlling field bindweed plants in agricultural crops which comprises applying to the plants or to the locus of the plants an effective amount of the fungus Phomopsis convolvulus to effect and produce typical lesions in the bindweed plants so as to inhibit the growth of or kill the bindweed plants. A plant growth regulating composition comprising a plant growth regulating effective amount of a culture of microorganisms of the species Phomopsis convolvulus in association with an agriculturally acceptable carrier. A biologically pure culture of the fungal microorganism having the identifying characteristics of Phomopsis convolvulus IMI 312959.
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公开(公告)号：EP0191607A3
公开(公告)日：1988-07-20
申请号：EP86300828
申请日：1986-02-06
摘要： A process for the improved culturing of filamentous basidiomycete fungi is provided. The improvement lies in adding to the culture medium a polymer additive which reduces the aggregation of the filaments of fungi. Preferred polymer additives are polyacrylate and polymethacrylate thickening agents.
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公开(公告)号：EP0133035A3
公开(公告)日：1986-08-13
申请号：EP84305116
申请日：1984-07-27
发明人： Takasawa, Seigo , Morikawa, Yasushi
IPC分类号： C12N09/42 , C12N01/14 , C12R01/885
CPC分类号： C12R1/885 , C12N9/2437 , C12Y302/01004 , Y10S435/945
摘要： Cellulase may be prepared in good yield and at relatively low cost by culturing certain mutant strains of the genus Trichoderma, which exhibit increased inducibility of cellulase by L-sorbose, in medium wherein the carbon source comprises cellulose-containing material of plant origin, for example, bagasses, waste papers, rice plant hulls and straws or soybean wastes. Preferred mutant strains are exemplified by Trichoderma reesei PC-1-4, PC-3-7, X-30 and X-31.
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9.发明公开Process for the production of acid protease, and strains of the Aspergillus-type moulds producing the same 失效生产酸性蛋白酶的方法，以及生产该类蛋白质的豆粕型菌株
公开(公告)号：EP0082395A3
公开(公告)日：1984-09-26
申请号：EP82111288
申请日：1982-12-06
IPC分类号： C12N09/62 , C12N01/14 , A23K01/165 , C12R01/685
CPC分类号： C12R1/685 , A23K20/189 , C12N9/62 , Y10S435/917
摘要： Saure Proteasen mit breitem Wirkungsspektrum werden in hohen Ausbeuten von selektierten Aspergillus-Mutanten erhalten.
Für die Selektion geht man von einem Wildstamm aus, bevorzugt Aspergillus niger var. Thienhem CBS 319.81, der extrazelluläre saure Protease bildet. Die Mutierung erfolgt vorzugweise durch UV-Bestrahlung. Mutanten mit erhöhter proteolytischer Aktivität lassen sich selektieren, indem man sie auf Caseinatagarplatten ausspatelt, dem Agarmedium einen Inhibitor für Carboxylprotease, bevorzugt Pepstatin, zugibt, bebrütet und Mutantenkolonien mit verstarkter caseinolytischer Hofbildung isoliert.
Beansprucht werden die Mutanten Aspergillus niger AP 114-III-69 bzw. -IV-70, -74, und -80 mit den Hinterlegungsnummern CBS 320.81; 321.81, 322.81 und 323.81, sowie der aus Boden isolierte Wildstamm Aspergillus niger var. Tienhem AP 114 (CBS 319.81) sowie die Verwendung der daraus gewonnen Protease als Tierfutterzusatz.-
公开(公告)号：EP0049410A3
公开(公告)日：1982-12-15
申请号：EP81107449
申请日：1981-09-19
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