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    ENZYMATIC SYNTHESIS OF ODILORHABDIN AND RELATED PEPTIDES/PROTEINS COMPRISING NON-STANDARD AMINO ACID RESIDUES
    4.
    发明公开
    ENZYMATIC SYNTHESIS OF ODILORHABDIN AND RELATED PEPTIDES/PROTEINS COMPRISING NON-STANDARD AMINO ACID RESIDUES 审中-公开

    公开(公告)号：EP4424821A1

    公开(公告)日：2024-09-04

    申请号：EP23159422.7

    申请日：2023-03-01

    申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

    发明人： The designation of the inventor has not yet been filed

    IPC分类号： C12N9/02 ,  A61P31/04 ,  C07C227/16

    CPC分类号： C12N9/0071 ,  A61P31/04 ,  C07C227/16

    摘要： The present invention relates to a novel method of preparing a non-standard amino acid or a peptide/protein comprising a non-standard amino acid residue, as appropriate, wherein said amino acid residue is selected from 2,4-diamino-3-hydroxybutyrate, L-5-hydroxylysine, L-3-hydroxyarginine, 2,3-didehydroarginine by using enzymatic transformations. Accordingly, the present invention further relates to a method of preparing a peptide/protein comprising one or more non-standard amino acids selected from 2,4-diamino-3-hydroxybutyrate, L-5-hydroxylysine, L-3-hydroxyarginine and 2,3-didehydroarginine. The methods of the present invention are particularly useful in enzyme-assisted preparation of odilorhabdins and their derivatives. The present invention further relates to a polypeptide having 2,4-diaminobutyrate-3-hydroxylase activity and its use for catalyzing a hydroxylation at position 3 of 2,4-diaminobutyrate, to a polypeptide having having L-arginine 3-hydroxylase activity and its use for catalyzing a hydroxylation at position 5 of L-lysine, to a polypeptide having L-arginine 3-hydroxylase activity and its use for for catalyzing a hydroxylation at position 3 of L-arginine, and to a polypeptide having a deacylase activity, which is preferably capable of removing fatty acyl from the N-terminus of an odilorhabdin, and its use for catalyzing a deacylation, preferably for removing a fatty acyl from the N-terminus of an odilorhabdin.