公开(公告)号：US20150344903A1

公开(公告)日：2015-12-03

申请号：US14820893

申请日：2015-08-07

Applicant: Terry Wright ,  Justin Lira ,  Donald Merlo ,  Nicole Arnold

Inventor： Terry Wright ,  Justin Lira ,  Donald Merlo ,  Nicole Arnold

IPC: C12N15/82

CPC classification number: C12N15/8274 ,  C12N9/0069 ,  C12N15/8209

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

Abstract translation: 本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和 改善除草剂抗性管理方案。 更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。 据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。 这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。 本发明还包括控制杂草的相关方法。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

公开(公告)号：US07838733B2

公开(公告)日：2010-11-23

申请号：US11587893

申请日：2005-05-02

Applicant: Terry Wright ,  Justin Lira ,  Donald Merlo ,  Nicole Arnold

Inventor： Terry Wright ,  Justin Lira ,  Donald Merlo ,  Nicole Arnold

IPC: C12N15/82 ,  C12N15/52

CPC classification number: C12N15/8274 ,  C12N9/0069 ,  C12N15/8209

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

Abstract translation: 本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括单独产生本发明的一种或多种酶或与另一种除草剂抗性基因“优选地基于草甘膦抗性”基因“堆叠”的植物，以便提供更广泛和更强大的杂草控制，增加的治疗灵活性，和 改善除草剂抗性管理方案。 更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。 据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。 这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。 本发明还包括控制杂草的相关方法。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

公开(公告)号：US20080102079A1

公开(公告)日：2008-05-01

申请号：US11925300

申请日：2007-10-26

Applicant: William Ainley ,  Janna Armstrong ,  Krishna Madduri ,  Donald Merlo ,  Kelley Smith ,  Mark Thompson ,  Steven Webb ,  Liu Shen

Inventor： William Ainley ,  Janna Armstrong ,  Krishna Madduri ,  Donald Merlo ,  Kelley Smith ,  Mark Thompson ,  Steven Webb ,  Liu Shen

IPC: A61K39/00 ,  A61P43/00 ,  C07K14/00 ,  C12N15/11 ,  C12P21/04

CPC classification number: A61K39/08 ,  A61K2039/552 ,  A61K2039/55577 ,  C07K14/33

Abstract: This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic Clostridium perfringens (Cp) α-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the Pseudomonas fluorescens system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

Abstract translation: 本发明部分涉及针对坏死性肠炎（NE）的家禽疫苗的开发。 疫苗使用作为突变的全长无毒产气荚膜梭菌（Cp）α-毒素蛋白（Mcpa）的保护性抗原。 例如，通过减少NE攻击路径中的病变严重程度来证明该疫苗的效用。 本文还公开了用于大量生产该疫苗的新方法。 一个例举的方法涉及在细菌表达系统中产生NE疫苗（突变的α毒素），优选利用荧光假单胞菌系统，用于商业用于控制家禽业中的NE。 本发明的疫苗可以以几种不同的方式优选施用于鸡。 还公开了来自Cp的鸡分离株的新型VI型α毒素。

公开(公告)号：US20060205653A1

公开(公告)日：2006-09-14

申请号：US11366918

申请日：2006-03-02

Applicant: Ignacio Larrinua ,  Aaron Woosley ,  Thomas Meade ,  Donald Merlo ,  Timothy Hey ,  Stephanie Burton

Inventor： Ignacio Larrinua ,  Aaron Woosley ,  Thomas Meade ,  Donald Merlo ,  Timothy Hey ,  Stephanie Burton

IPC: A01N65/00 ,  C07H21/04 ,  C12P21/06 ,  C12N15/74 ,  C12N1/14 ,  C12N1/18 ,  C07K14/37

CPC classification number: C07K14/195 ,  A01N63/00 ,  A01N63/04 ,  C07K14/20 ,  C07K14/21 ,  C07K14/37 ,  C07K16/14 ,  C07K2319/00 ,  Y10S530/858

Abstract: The subject invention provides exciting new sources for surprising, new types of toxin complex (“TC”) proteins. The subject invention includes these new classes and types of TC proteins. The subject invention also includes polynucleotides that encode the subject proteins. The subject invention further provides vectors and cells comprising these polynucleotides. The subject invention also provides novel methods of controlling insects. The subject invention relates in part to the surprising discovery that new types of TC proteins can be obtained from a widely diverse phylogenetic spectrum of organisms including, most notably and surprisingly, eukaryotic fungus.

Abstract translation: 本发明为令人惊奇的新型毒素复合物（“TC”）蛋白质提供令人兴奋的新来源。 本发明包括这些新类型和TC蛋白。 本发明还包括编码受试蛋白的多核苷酸。 本发明进一步提供了包含这些多核苷酸的载体和细胞。 本发明还提供了控制昆虫的新方法。 本发明部分地涉及令人惊奇的发现，即可以从广泛多样的生物系统发育谱获得新型TC蛋白质，包括最显着和令人惊讶的真核真菌。

公开(公告)号：US20060168683A1

公开(公告)日：2006-07-27

申请号：US11070573

申请日：2005-03-02

Applicant: Timothy Hey ,  Thomas Meade ,  Stephanie Burton ,  Donald Merlo ,  Qihua Cai ,  Haley Moon ,  Joel Sheets ,  Aaron Woosley

Inventor： Timothy Hey ,  Thomas Meade ,  Stephanie Burton ,  Donald Merlo ,  Qihua Cai ,  Haley Moon ,  Joel Sheets ,  Aaron Woosley

IPC: A01H1/00 ,  C07K14/415 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N15/8286 ,  C07K14/195 ,  C07K14/24 ,  C07K14/32 ,  C07K2319/00 ,  C07K2319/55 ,  C12N15/62 ,  Y02A40/162

Abstract: The subject invention relates to insecticidal toxin complex (“TC”) fusion proteins and to polynucleotides that encode these fusion proteins. The subject invention also includes polynucleotides that encode the subject TC fusion proteins, and vectors comprising said polynucleotides. In some embodiments, the invention provides a fusion protein comprising a Class A protein, a Class B protein, and a Class C TC protein fused together to form a single protein. In some other embodiments, the invention provides a fusion protein comprising a Class B and a Class C TC proteins fused together. In the latter embodiments, the BC or CB fusion protein can be used to enhance or potentiate the anti-insect activity of a “Toxin A” or Class A protein. Heretofore, there was no expectation that such fusion proteins would properly function and retain their activity when fused together. The subject invention advantageously reduces the number of genes needed to transform plants. Thus, the subject invention also includes plants, cells (bacterial and plant cells for example), and seeds that comprise said polynucleotides. Said plants can produce fusion proteins of the subject invention, which convey insect resistance to said plants. The subject invention also includes methods of controlling pests (preferably insects and other plant pests) with fusion proteins of the subject invention.

Abstract translation: 本发明涉及杀虫毒素复合物（“TC”）融合蛋白和编码这些融合蛋白的多核苷酸。 本发明还包括编码受试者TC融合蛋白的多核苷酸和包含所述多核苷酸的载体。 在一些实施方案中，本发明提供了融合蛋白，其包含一起融合以形成单一蛋白质的A类蛋白质，B类蛋白质和C类TC蛋白质。 在一些其他实施方案中，本发明提供了融合蛋白，其包含融合在一起的B类和C类TC蛋白。 在后一个实施方案中，BC或CB融合蛋白可用于增强或增强“毒素A”或A类蛋白的抗昆虫活性。 迄今为止，没有任何期望，当融合在一起时，这种融合蛋白将适当地起作用并保持其活性。 本发明有利地减少转化植物所需的基因数量。 因此，本发明还包括植物，细胞（例如细菌和植物细胞）和包含所述多核苷酸的种子。 所述植物可产生本发明的融合蛋白，其向所述植物传递昆虫抗性。 本发明还包括用本发明的融合蛋白控制害虫（优选昆虫和其它植物害虫）的方法。

公开(公告)号：US20050102713A1

公开(公告)日：2005-05-12

申请号：US10703280

申请日：2003-11-07

Applicant: Donald Merlo ,  Dong Liu ,  Stephanie Burton ,  Todd Glancy

Inventor： Donald Merlo ,  Dong Liu ,  Stephanie Burton ,  Todd Glancy

IPC: A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N5/10 ,  C12N15/29 ,  C12N15/82 ,  C12Q1/68

CPC classification number: C12N15/8216 ,  C07H21/04

Abstract: The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5′ and 3′ untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

Abstract translation: 本发明提供用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。 根据本发明，获得核酸序列和/或衍生自编码osmotin蛋白的基因的5'和3'非翻译区，并被工程改造成位于载体的选定编码区的相应部分。 载体构建体可以通过常规方法引入植物和/或植物组织中，导致所选编码区的表达增强。 在优选的实施方案中，选择的编码区是表达一种或多种已知赋予转基因植物和/或植物组织杀虫活性水平的蛋白质的嵌合基因或基因片段。

公开(公告)号：US20080019914A1

公开(公告)日：2008-01-24

申请号：US11775663

申请日：2007-07-10

Applicant: Scott Bintrim ,  Scott Bevan ,  Baolong Zhu ,  Donald Merlo

Inventor： Scott Bintrim ,  Scott Bevan ,  Baolong Zhu ,  Donald Merlo

IPC: A01N63/00 ,  A01P7/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/32 ,  C12N1/20 ,  C12N1/21 ,  C12N5/04

CPC classification number: C12P19/34 ,  C07K14/195 ,  C12P21/02 ,  C12Q1/689 ,  C12Q2600/142

Abstract: The subject invention provides unique biological alternatives for pest control. More specifically, the present invention relates to novel pesticidal proteins, novel sources of pesticidal proteins, polynucleotides that encode such toxins, and to methods of using these toxins to control insects and other plant pests. The subject invention relates to the surprising discovery that Paenibacillus species, and proteins therefrom, have toxicity to lepidopterans. There have been no known reports of a Paenibacillus species, strain, or protein having toxicity to lepidopterans. This is also the first known example of a Paenibacillus Cry protein that is toxic to lepidopterans. Furthermore, this is the first known report of a Paenibacillus having toxin complex (TC)-like proteins. The DAS1529 isolate disclosed here is also the first known example of a natural bacterium that produces both a Cry toxin and TC proteins. The subject invention also relates to new classes of Cry and TC proteins that are pesticidally active.

Abstract translation: 本发明提供了用于病虫害防治的独特的生物学替代物。 更具体地，本发明涉及新型杀虫蛋白，杀虫蛋白的新来源，编码这种毒素的多核苷酸，以及使用这些毒素来控制昆虫和其它植物害虫的方法。 本发明涉及令​​人惊奇的发现：Paenibacillus物种及其蛋白质对鳞翅目病毒具有毒性。 没有已知的具有对鳞翅目昆虫具有毒性的Paenibacillus物种，菌株或蛋白质的报道。 这也是对鳞翅目昆虫具有毒性的第一个已知的Paenibacillus Cry蛋白的例子。 此外，这是具有毒素复合物（TC）样蛋白质的Paenibacillus的第一个已知报告。 此处公开的DAS1529分离物也是产生Cry毒素和TC蛋白质的天然细菌的第一已知实例。 本发明还涉及具有杀虫活性的新类型的Cry和TC蛋白。