公开(公告)号：US20060012785A1

公开(公告)日：2006-01-19

申请号：US10967330

申请日：2004-10-19

Applicant: Joerg-Michael Funk ,  Ralf Wolleschensky ,  Joerg Steinert

Inventor： Joerg-Michael Funk ,  Ralf Wolleschensky ,  Joerg Steinert

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01J3/06 ,  G01J3/02 ,  G01J3/0202 ,  G01J3/021 ,  G01J3/0227 ,  G01J3/0237 ,  G01J3/10 ,  G01J3/44 ,  G01J2003/1217 ,  G02B21/0044 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/008

Abstract: In a confocal laser scanning microscope for Raman spectroscopy with an illuminating configuration (2), which provides an illuminating beam for illuminating a probe region (23), with a scanning configuration (3, 4), which guides the illuminating beam over the probe while scanning, and with a detector configuration (5), which via the scanning configuration (3, 4) images the illuminated probe region (23) by means of a confocal aperture (26) on to at least one detector unit (28), it is provided that the illuminating configuration (2) of the scanning configuration (3, 4) provides a line-shaped illuminating beam, that the scanning configuration (3, 4) guides the line-shaped illuminating beam over the probe f while scanning and that the confocal aperture is designed as a slotted aperture (26) or as a slot-shaped region (28, 48) of the detector unit (28) acting as a confocal aperture.

Abstract translation: 在具有照明配置（2）的拉曼光谱的共焦激光扫描显微镜中，其提供用于照射探针区域（23）的照明光束，其具有扫描配置（3,4），其将照射光束引导到探针上，同时 扫描和检测器配置（5），其经由扫描配置（3,4）通过共焦孔（26）将照射的探测区域（23）图像到至少一个检测器单元（28）上， 提供扫描配置（3,4）的照明配置（2）提供线形照明光束，扫描配置（3,4）在扫描时将线形照明光束引导到探头f上，而在扫描结构 共焦孔被设计为开槽孔（26）或作为用作共焦孔的检测器单元（28）的槽形区域（28,48）。

公开(公告)号：US20060011858A1

公开(公告)日：2006-01-19

申请号：US10967328

申请日：2004-10-19

Applicant: Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

Inventor： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC: G01N21/64

CPC classification number: G02B21/0076 ,  G02B21/0048

Abstract: Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanning means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

Abstract translation: 用于通过第一扫描装置沿着基本上垂直于照明轴线的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样品区域的方法，其中与至少一个样品区域的平面成角度 相对运动，优选地与其垂直，第二扫描装置被移动，并且通过被耦合的第一和第二扫描装置的运动而进行图像采集，并且三维采样运动是通过样品的照射完成的，其中第二扫描装置 耦合到第一扫描装置的运动，使得沿着第一扫描装置的至少一个扫描方向延伸的直线和/或曲线和/或平面和/或曲面被扫描，以及沿着 第二扫描装置的扫描方向。

公开(公告)号：US20120268749A1

公开(公告)日：2012-10-25

申请号：US13299515

申请日：2011-11-18

Applicant: Michael Goelles ,  Ralf Netz ,  Frank Hecht ,  Joerg-Michael Funk

Inventor： Michael Goelles ,  Ralf Netz ,  Frank Hecht ,  Joerg-Michael Funk

IPC: G01B11/14

CPC classification number: G01B11/14 ,  G02B21/0036 ,  G02B21/008

Abstract: Laser scanning microscope and its operating method in which at least two first and second scanning systems activated independently of each other and that can move in at least one direction illuminate a sample with the help of a beam-combining element, and the light is detected by the sample as it comes in, The scanning fields created by the light distributions on the sample mutually overlap to create a reference pattern on the sample with one of the light distributions, which is then captured and used to create the overlap using the second light distribution and/or a reference pattern arranged in the sample plane or in an intermediate image plane is captured by both scanning fields and used to create the overlap and/or structural characteristics of the sample are captured by the two scanning fields as reference pattern and used to create the overlap in which correction values are determined.

Abstract translation: 激光扫描显微镜及其操作方法，其中至少两个第一和第二扫描系统彼此独立地激活并且可以在至少一个方向上移动，借助于光束组合元件照射样品，并且光被检测到 样品进入，由样品上的光分布产生的扫描场相互重叠，以在样品上产生参考图案，其中一个光分布被捕获并用于使用第二光分布来创建重叠 和/或布置在采样平面或中间图像平面中的参考图案由两个扫描场捕获并用于创建样本的重叠和/或结构特征由两个扫描场作为参考图案捕获，并用于 创建确定校正值的重叠。

公开(公告)号：US20100097694A1

公开(公告)日：2010-04-22

申请号：US12578202

申请日：2009-10-13

Applicant: Bernhard Zimmermann ,  Ralf Netz ,  Frank Hecht ,  Joerg-Michael Funk ,  Ralf Engelmann

Inventor： Bernhard Zimmermann ,  Ralf Netz ,  Frank Hecht ,  Joerg-Michael Funk ,  Ralf Engelmann

IPC: G02B21/06

CPC classification number: G02B21/06 ,  G02B21/00 ,  G02B21/0032 ,  G02B21/0076 ,  G02B27/0972

Abstract: Method for actuation control of a microscope, in particular of a Laser Scanning Microscope, in which, at least one first illumination light, preferably moving at least in one direction, as well as at least one second illumination light moving at least in one direction, illuminate a sample through a beam combiner, a detection of the light coming from the sample takes place, whereby, at least one part of the illumination light is generated through the splitting of the light from a common illuminating unit, characterized in that, by means of a common control unit, a controlled splitting into the first and the second illumination light takes place, in which the intensity of the first illuminating light, specified by the user or specified automatically, is assigned a higher priority (is prioritized) compared to the specified value for the second illumination light, and an adjustment for the second illumination light takes place until a maximum value is obtained, which is determined by the value specified for the first illumination light.

Abstract translation: 显微镜，特别是激光扫描显微镜的致动控制方法，其中至少一个至少在一个方向上移动的第一照明光以及至少沿一个方向移动的至少一个第二照明光， 通过光束组合器照射样品，发生来自样品的光的检测，由此通过从公共照明单元分离光而产生照明光的至少一部分，其特征在于，通过装置 发生公共控制单元的受控分割，进入第一和第二照明光，其中由用户指定或自动指定的第一照明光的强度被分配为比较高的优先级（优先化） 对于第二照明光的指定值，并且进行第二照明光的调整，直到获得最大值，其由v 为第一个照明灯指定。

公开(公告)号：US20090046360A1

公开(公告)日：2009-02-19

申请号：US11987218

申请日：2007-11-28

Applicant: Joerg-Michael Funk ,  Ralf Wolleschensky ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Ralf Engelmann

Inventor： Joerg-Michael Funk ,  Ralf Wolleschensky ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Ralf Engelmann

IPC: G02B21/06

CPC classification number: G02B21/0032

Abstract: Raster scanning light microscope with line pattern scanning with at least one illumination module, in which the means to achieve a variable partition of the laser light into least two illumination channels are envisioned and joint illumination of a sample takes place at the same or at different areas of the sample.

Abstract translation: 具有至少一个照明模块的线阵扫描的光栅扫描光学显微镜，其中实现将激光可变分割成至少两个照明通道的装置，并且在相同或不同的区域发生样品的联合照明 的样品。

公开(公告)号：US20070159689A1

公开(公告)日：2007-07-12

申请号：US10586137

申请日：2005-03-24

Applicant: Dieter Schau ,  Peter Huehn ,  Michael Goelles ,  Joerg-Michael Funk

Inventor： Dieter Schau ,  Peter Huehn ,  Michael Goelles ,  Joerg-Michael Funk

IPC: G02B21/00

CPC classification number: G02B21/002 ,  G02B21/248

Abstract: Arrangement for changing the uncoupling of the object beam and/or the coupling of the beam in a Laser Scanning Microscope, whereby a rotatable tube revolver is provided, which has at least four switching positions and each tube position is associated with a separate tube lens as well as deflecting elements for the object beam, whereby the tube positions for at least the LSM operation, LSM/NDD and/or manipulation and/or illumination, camera and visual operation modes are provided.

Abstract translation: 用于在激光扫描显微镜中改变物体束的解耦和/或光束的耦合的装置，由此提供可旋转的管旋转枪，其具有至少四个切换位置，并且每个管位置与单独的管透镜相关联 以及用于物体光束的偏转元件，由此提供用于至少LSM操作，LSM / NDD和/或操纵和/或照明，照相机和视觉操作模式的管位置。

公开(公告)号：US20060012871A1

公开(公告)日：2006-01-19

申请号：US10967638

申请日：2004-10-19

Applicant: Joerg-Michael Funk ,  Ralf Wolleschensky ,  Joerg Steinert

Inventor： Joerg-Michael Funk ,  Ralf Wolleschensky ,  Joerg Steinert

IPC: G02B21/06

CPC classification number: G02B21/0044 ,  G01J3/02 ,  G01J3/0202 ,  G01J3/021 ,  G01J3/0227 ,  G01J3/0237 ,  G01J3/06 ,  G01J3/10 ,  G01J3/44 ,  G01J2003/1217 ,  G02B21/0064 ,  G02B21/008

Abstract: In a confocal laser scanning microscope with an illuminating configuration (2), which provides an illuminating beam for illuminating a probe region (23), with a scanning configuration (3, 4), which guides the illuminating beam over the probe while scanning, and with a detector configuration (5), which via the scanning configuration (3, 4) images the illuminated probe region (23) by means of a confocal aperture (26) on to at least one detector unit (28), it is provided that the illuminating configuration (2) of the scanning configuration (3, 4) provides a line-shaped illuminating beam, that the scanning configuration (3, 4) guides the line-shaped illuminating beam over the probe f while scanning and that the confocal aperture is designed as a slotted aperture (26) or as a slot-shaped region (28, 48) of the detector unit (28) acting as a confocal aperture.

Abstract translation: 在具有照射配置（2）的共聚焦激光扫描显微镜中，其提供用于照射探针区域（23）的照明光束，所述照射光束具有扫描配置（3,4），其在扫描时将照射光束引导到探测器上;以及 具有检测器配置（5），其通过扫描配置（3,4）通过共焦孔（26）将照射的探测区域（23）映射到至少一个检测器单元（28）上， 扫描配置（3,4）的照明配置（2）提供线形照明光束，扫描配置（3,4）在扫描时将线形照明光束引导到探头f上，并且共焦孔 被设计为作为共焦孔的检测器单元（28）的开槽孔（26）或槽形区域（28,48）。

公开(公告)号：US20050063051A1

公开(公告)日：2005-03-24

申请号：US10888186

申请日：2004-07-09

Applicant: Ralph Lange ,  Stefan Wilhelm ,  Volker Gerstner ,  Roland Scheler ,  Michael Goelles ,  Joerg-Michael Funk

Inventor： Ralph Lange ,  Stefan Wilhelm ,  Volker Gerstner ,  Roland Scheler ,  Michael Goelles ,  Joerg-Michael Funk

IPC: G02B21/18 ,  G02B21/00 ,  G02B21/06

CPC classification number: G02B21/002 ,  G02B21/06

Abstract: An arrangement for direct input coupling of a laser, preferably of a short-pulse laser, for nonlinear sample excitation which is located outside of the microscope housing, into the beam path of a laser scanning microscope (LSM), comprising a housing in which is integrated an adjusting laser that can be coupled into the microscope beam path by beam splitters, wherein the housing is advantageously integrated so as to be insertable in the scan module of the LSMA, and a beam splitter for the direct coupling laser has high transmission in the direction of the objective and, further, partial transmission for back-reflections of the adjusting laser coming from the specimen for adjusting the overlapping of the adjusting laser and direct coupling laser.

Abstract translation: 用于直接输入耦合激光扫描显微镜（LSM）的激光扫描显微镜（LSM）的光束路径中的用于将位于显微镜外壳之外的非线性样品激发的短脉冲激光器的激光直接输入耦合的装置，其中， 集成了可以通过分束器耦合到显微镜光束路径中的调节激光器，其中壳体有利地集成以便可插入到LSMA的扫描模块中，并且用于直接耦合激光器的分束器在 并且进一步地，用于调整来自测试样品的调节激光器的反射反射的部分透射以调节调节激光器和直接耦合激光器的重叠。

公开(公告)号：US08780443B2

公开(公告)日：2014-07-15

申请号：US12578202

申请日：2009-10-13

Applicant: Ralf Engelmann ,  Joerg Michael Funk ,  Bernhard Zimmerman ,  Ralph Netz ,  Frank Hecht

Inventor： Ralf Engelmann ,  Joerg Michael Funk ,  Bernhard Zimmerman ,  Ralph Netz ,  Frank Hecht

IPC: G02B21/06 ,  G02B27/14 ,  G02B27/09 ,  G02B21/00

CPC classification number: G02B21/06 ,  G02B21/00 ,  G02B21/0032 ,  G02B21/0076 ,  G02B27/0972

Abstract: Method for actuation control of a microscope, in particular of a Laser Scanning Microscope, in which, at least one first illumination light, preferably moving at least in one direction, as well as at least one second illumination light moving at least in one direction, illuminate a sample through a beam combiner, a detection of the light coming from the sample takes place, whereby, at least one part of the illumination light is generated through the splitting of the light from a common illuminating unit, characterized in that, by means of a common control unit, a controlled splitting into the first and the second illumination light takes place, in which the intensity of the first illuminating light, specified by the user or specified automatically, is assigned a higher priority (is prioritized) compared to the specified value for the second illumination light, and an adjustment for the second illumination light takes place until a maximum value is obtained, which is determined by the value specified for the first illumination light.

Abstract translation: 显微镜，特别是激光扫描显微镜的致动控制方法，其中，至少一个至少在一个方向上移动的第一照明光以及至少沿一个方向移动的至少一个第二照明光， 通过光束组合器照射样品，发生来自样品的光的检测，由此通过从公共照明单元分离光而产生照明光的至少一部分，其特征在于，通过装置 发生公共控制单元的受控分割，进入第一和第二照明光，其中由用户指定或自动指定的第一照明光的强度被分配为比较高的优先级（优先化） 对于第二照明光的指定值，并且进行第二照明光的调整，直到获得最大值，其由v 为第一个照明灯指定。

公开(公告)号：US07656583B2

公开(公告)日：2010-02-02

申请号：US11783289

申请日：2007-04-06

Applicant: Michael Goelles ,  Ralf Netz ,  Marcus Heidkamp ,  Joerg-Michael Funk

Inventor： Michael Goelles ,  Ralf Netz ,  Marcus Heidkamp ,  Joerg-Michael Funk

IPC: G02B27/10

CPC classification number: G02B27/144 ,  G02B21/0064

Abstract: A beam corradiator for combining two radiation beams, preferably movable beams independent from each other in at least one direction, to scan and/or influence a sample, preferably a manipulation system and an imaging system, with a partially reflecting layer being provided for the corradiation, wherein the thickness of the layer is provided with a preferably consistent incline or decline over the optically effective cross-section of the beam corradiatior.

Abstract translation: 一种用于组合两个辐射束，优选在至少一个方向上彼此独立的可移动光束以扫描和/或影响样品，优选地操纵系统和成像系统的光束校正器，其中提供了部分反射层用于辐照 ，其中所述层的厚度在所述光束辐射体的光学有效横截面上被提供优选地一致的倾斜或下降。