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1.发明授权公开(公告)号：US09207235B2
公开(公告)日：2015-12-08
申请号：US13575564
申请日：2010-12-01
申请人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
发明人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
CPC分类号： G01N33/54313 , B01L3/502715 , B01L2200/0668 , B01L2300/0609 , B01L2300/0877 , B01L2300/0883 , G01N21/6452 , G01N21/648 , G01N33/5308 , G01N33/54373 , G01N33/582 , G01N35/00 , G01N2035/00564
摘要： Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).
摘要翻译： 提供了用于核酸分析的反应装置，其中携带已被固定在其上的被检测核酸的微粒根据二维传感器的像素尺寸以格子形式排列在基板上。 通过在基板（101）上设置有通道形成反应室的核酸分析用反应装置，检测固定在基板（101）上的微粒（103）上的核酸。 携带已被固定在其上的被检测核酸的微粒（103）通过在基板（101）上排列的微结构（102）布置。
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公开(公告)号：US09150911B2
公开(公告)日：2015-10-06
申请号：US13696962
申请日：2011-04-20
IPC分类号： C12Q1/68 , B01L3/00 , G01N27/447 , G01N35/10
CPC分类号： C12Q1/6844 , B01L3/502746 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0877 , B01L2300/0887 , B01L2400/086 , G01N27/447 , G01N35/1095
摘要： A nucleic acid analysis reaction cell and a nucleic acid analyzer are provided, in which a uniform flow rate is realized, so that a portion where a flow rate is low is removed and washing time for reagent removal is shortened.A flow path (103) is provided which includes a detection area (108) for detecting sequence information of a nucleic acid fragment and detection outer areas (107) disposed at both ends of the detection area (108). An inflow port (105) is provided in one of the detection outer areas (107) and a discharge port (106) is provided in the other of the detection outer areas (107). The detection outer areas (107) disposed at both the ends of the detection area (108) are areas whose widths become narrow toward ends, and guides (104) for branching a liquid are provided in at least the detection outer area (107) in which the inflow port (105) is provided.
摘要翻译： 提供了一种核酸分析反应池和核酸分析仪，其中实现了均匀的流速，从而消除了流速低的部分，缩短了除去试剂的洗涤时间。 提供了一种流路（103），其包括用于检测核酸片段的序列信息的检测区域（108）和设置在检测区域（108）两端的检测外部区域（107）。 在一个检测外部区域（107）中设置有流入口（105），并且在另一个检测外部区域（107）中设置有排出口（106）。 设置在检测区域（108）的两端的检测外部区域（107）是宽度朝向端部变窄的区域，并且至少在检测外部区域（107）中设置用于分支液体的引导件（104） 其中设置有流入口（105）。
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3.发明授权Apparatus and method for detecting target substance, or device used for these apparatus and method 有权用于检测目标物质的装置和方法，或用于这些装置和方法的装置公开(公告)号：US08228505B2
公开(公告)日：2012-07-24
申请号：US12233184
申请日：2008-09-18
IPC分类号： G01N21/55
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a material other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
摘要翻译： 本发明的目的是检测具有高对比度的目标物质。 本发明涉及使用透光基板和用于诱导等离子体共振的金属的目标物质的分析，并且还使用具有与基板形成界面的开口部分的低折射率层，并且具有较低的折射率 指数比底物。 从基板侧发射的光在界面处被全反射，以照射具有ev逝光的开口部中的金属。 检测由目标物质通过ev逝光的等离子体共振产生的光。 根据本发明，可以减少对目标物质以外的材料的ev逝光的照射，从而可以减少来自目标物质以外的武器例如悬浮在目标物质周围的分子的发光。
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公开(公告)号：US20120130050A1
公开(公告)日：2012-05-24
申请号：US13388671
申请日：2010-07-29
CPC分类号： G01N33/54353 , A61K2039/625 , G01N33/543 , G01N33/54393
摘要： This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.
摘要翻译： 本发明提供一种生物分子修饰底物，其包含选择性地固定在其上给定区域上的生物分子。 生物分子修饰基质包括：至少包含第一表面和第二表面的基底; 第一连接分子，其包含烃链和能够选择性地结合到烃链一端的第一表面的官能团，其通过该官能团与第一表面结合; 第二连接分子，其包含能够结合第一连接分子的烃链的反应性基团，其通过反应性基团和烃链之间的键与第一连接分子结合; 和通过第二连接分子与其结合的生物分子。
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公开(公告)号：US20090023202A1
公开(公告)日：2009-01-22
申请号：US12173472
申请日：2008-07-15
IPC分类号： C12M1/34
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/6452 , G01N2021/058 , G01N2021/6439 , Y10S435/808
摘要： The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.
摘要翻译： 本发明涉及用于通过荧光测定分析样品中的核酸的核酸分析装置，其中通过光照射进行局部表面等离子体激光，并且其中用于核酸分析的核酸探针或核酸合酶 在样品中放置在产生表面等离子体的区域中。 本发明允许有效地产生表面等离子体激元的荧光增强效果，并且还能够在发挥荧光增强效果的区域中固定DNA探针或核酸合酶，从而可以进行 基本伸长反应的测量，而不用荧光分子去除未反应的底物。
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公开(公告)号：US09523124B2
公开(公告)日：2016-12-20
申请号：US13996638
申请日：2011-12-13
申请人： Masatoshi Narahara , Taro Nakazawa , Ryoji Inaba
发明人： Masatoshi Narahara , Taro Nakazawa , Ryoji Inaba
CPC分类号： C12Q1/6869 , B01J2219/00286 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/5027 , B01L2200/12 , B01L2300/0636 , B01L2300/0877 , B01L2300/0887
摘要： Sample nucleic acids are prevented from removing from a sample-immobilizing layer during a sequencing reaction. A reaction device for nucleic acid analysis is provided that enables high throughput analysis by increasing the number of nucleic acid samples that can be analyzed. The reaction device for nucleic acid analysis comprises a substrate, a sample-immobilizing layer on the substrate, nucleic acid samples or carriers having nucleic acid samples on their surfaces, which are immobilized on the sample-immobilizing layer, and a blocking layer that covers areas other than areas where the nucleic acid samples or the carriers are bound to the sample-immobilizing layer, wherein the immobilizing layer is formed of an inorganic oxide.
摘要翻译： 在测序反应期间，防止样品核酸从样品固定层中除去。 提供了用于核酸分析的反应装置，其通过增加可分析的核酸样品的数量来实现高通量分析。 用于核酸分析的反应装置包括底物，底物上的样品固定层，其表面上具有固定在样品固定层上的核酸样品的核酸样品或载体，以及覆盖区域的阻挡层 除了核酸样品或载体与样品固定层结合的区域以外，其中固定层由无机氧化物形成。
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公开(公告)号：US08865403B2
公开(公告)日：2014-10-21
申请号：US12997469
申请日：2009-05-13
CPC分类号： G01N21/648
摘要： An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.
摘要翻译： 本发明的目的在于从未反应的底物的荧光团区分与通过核酸合成并入探针中的核苷酸连接的单个荧光团。 本发明涉及通过荧光分析样品中的核酸的核酸分析装置，其中通过照射产生局部表面等离子体激元，并且用于分析样品中的核酸的探针位于表面等离子体为 生成。 根据本发明，由于可以有效地产生由于表面等离子体激元引起的荧光增强作用并且将探针固定在荧光增强作用范围内的区域，因此可以测量核酸合成而不去除未反应的 附着有荧光团的核苷酸。
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公开(公告)号：US20130084629A1
公开(公告)日：2013-04-04
申请号：US13696962
申请日：2011-04-20
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , B01L3/502746 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0877 , B01L2300/0887 , B01L2400/086 , G01N27/447 , G01N35/1095
摘要： A nucleic acid analysis reaction cell and a nucleic acid analyzer are provided, in which a uniform flow rate is realised, so that a portion where a flow rate is low is removed and washing time for reagent removal is shortened.A flow path (103) is provided which includes a detection area (108) for detecting sequence information of a nucleic acid fragment and detection outer areas (107) disposed at both ends of the detection area (108). An inflow port (105) is provided in one of the detection outer areas (107) and a discharge port (106) is provided in the other of the detection outer areas (107). The detection outer areas (107) disposed at both the ends of the detection area (108) are areas whose widths become narrow toward ends, and guides (104) for branching a liquid are provided in at least the detection outer area (107) in which the inflow port (105) is provided.
摘要翻译： 提供了一种核酸分析反应池和核酸分析仪，其中实现了均匀的流速，从而消除了流速低的部分，缩短了除去试剂的洗涤时间。 提供了一种流路（103），其包括用于检测核酸片段的序列信息的检测区域（108）和设置在检测区域（108）两端的检测外部区域（107）。 在一个检测外部区域（107）中设置有流入口（105），并且在另一个检测外部区域（107）中设置有排出口（106）。 设置在检测区域（108）的两端的检测外部区域（107）是宽度朝向端部变窄的区域，并且至少在检测外部区域（107）中设置用于分支液体的引导件（104） 其中设置有流入口（105）。
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9.发明申请Nucleic Acid Analyzer, Reaction Device for Nucleic Acid Analysis and Substrate of Reaction Device for Nucleic Acid Analysis 有权核酸分析仪，用于核酸分析的反应装置和用于核酸分析的反应装置的底物公开(公告)号：US20120316087A1
公开(公告)日：2012-12-13
申请号：US13575564
申请日：2010-12-01
申请人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
发明人： Yoshiaki Sugimura , Masatoshi Narahara , Kazumichi Imai , Toshiro Saito , Ryoji Inaba , Takuya Matsui
IPC分类号： C40B60/10
CPC分类号： G01N33/54313 , B01L3/502715 , B01L2200/0668 , B01L2300/0609 , B01L2300/0877 , B01L2300/0883 , G01N21/6452 , G01N21/648 , G01N33/5308 , G01N33/54373 , G01N33/582 , G01N35/00 , G01N2035/00564
摘要： Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).
摘要翻译： 提供了用于核酸分析的反应装置，其中携带已被固定在其上的被检测核酸的微粒根据二维传感器的像素尺寸以格子形式排列在基板上。 通过在基板（101）上设置有通道形成反应室的核酸分析用反应装置，检测固定在基板（101）上的微粒（103）上的核酸。 携带已被固定在其上的被检测核酸的微粒（103）通过在基板（101）上排列的微结构（102）布置。
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10.发明申请APPARATUS AND METHOD FOR DETECTING TARGET SUBSTANCE, OR DEVICE USED FOR THESE APPARATUS AND METHOD 有权用于检测目标物质的装置和方法或用于这些装置和方法的装置公开(公告)号：US20090079988A1
公开(公告)日：2009-03-26
申请号：US12233184
申请日：2008-09-18
CPC分类号： G01N21/648 , G01N21/6428
摘要： An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a martial other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.
摘要翻译： 本发明的目的是检测具有高对比度的目标物质。 本发明涉及使用透光基板和用于诱导等离子体共振的金属的目标物质的分析，并且还使用具有与基板形成界面的开口部分的低折射率层，并且具有较低的折射率 指数比底物。 从基板侧发射的光在界面处被全反射，以照射具有ev逝光的开口部中的金属。 检测由目标物质通过ev逝光的等离子体共振产生的光。 根据本发明，可以减少对目标物质以外的其他武器的ev逝光的照射，从而可以减少来自与目标物质以外的武装物（例如，悬浮在目标物质周围的分子）的发光。
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