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    Microfluidic electrophoresis chip having flow-retarding structure
    1.
    发明申请
    Microfluidic electrophoresis chip having flow-retarding structure 审中-公开
    具有阻流结构的微流控电泳芯片

    公开(公告)号：US20060042948A1

    公开(公告)日：2006-03-02

    申请号：US10932078

    申请日：2004-09-02

    申请人： Juan Santiago ,  Byoungsok Jung ,  Rajiv Bharadwaj

    发明人： Juan Santiago ,  Byoungsok Jung ,  Rajiv Bharadwaj

    IPC分类号： C07K1/26 ,  G01N27/447

    CPC分类号： G01N27/44791

    摘要： A capillary electrophoresis device and separation protocol uses a hydraulic resistance-providing structure (HRPS) in the main separation channel to separate the divide the main separate channel into an upstream portion and a downstream portion. The HRPS may take the form of a porous plug, or a solid plug provided with at least one shallow channel. A sample separates and migrates through the porous structure or the shallow channel, upon application of a voltage difference between the upstream and downstream sides. Among other things, the HRPS helps reduce electrokinetic flow in the presence of conductivity gradients and facilitates robust, high-gradient on-chip field amplified sample stacking. The HRPS also enables the use of a pressure-injection scheme for the introduction of a high conductivity gradient in a separation channel and thereby avoids flow instabilities associated with high conductivity gradient electrokinetics. The approach also allows for the suppression of electroosmotic flow (EOF) and benefits from the associated minimization of sample dispersion caused by non-uniform EOF mobilities. An injection procedure employing a single pressure-flow high-conductivity buffer injection step followed by standard high voltage control of electrophoretic fluxes of sample, may be employed.

    摘要翻译： 毛细管电泳装置和分离方案在主分离通道中使用液压阻力提供结构（HRPS），以将主分离通道分成上游部分和下游部分。 HRPS可以采用多孔插塞的形式，或者具有至少一个浅沟道的实心插头。 当上游侧和下游侧之间施加电压差时，样品分离并迁移通过多孔结构或浅沟道。 除了别的以外，HRPS有助于在电导率梯度的存在下减少电动流动，并且有助于稳健的高梯度片上场扩增样品堆叠。 HRPS还能够使用压力注入方案来在分离通道中引入高电导率梯度，从而避免与高电导率梯度电动力相关联的流动不稳定性。 该方法还允许抑制电渗流（EOF），并从由非均匀EOF迁移率引起的样品分散的相关最小化中获益。 可以采用使用单一压力 - 流动高电导率缓冲液注入步骤的注射程序，然后进行样品电泳通量的标准高压控制。

    High-throughput single-cell imaging, sorting, and isolation
    3.
    发明授权
    High-throughput single-cell imaging, sorting, and isolation 有权
    高通量单细胞成像，分选和分离

    公开(公告)号：US08934700B2

    公开(公告)日：2015-01-13

    申请号：US13783767

    申请日：2013-03-04

    申请人： Rajiv Bharadwaj ,  Bahram Fathollahi

    发明人： Rajiv Bharadwaj ,  Bahram Fathollahi

    IPC分类号： G06K9/00 ,  G06T7/00 ,  B01L3/00 ,  C12M1/00 ,  C12N5/00 ,  G01N33/53

    CPC分类号： H04N5/2251 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  C12M47/04 ,  C12N5/0081 ,  C12N5/0087 ,  G02B21/365 ,  G06T7/0012 ,  H04N5/2256 ,  H04N7/18

    摘要： The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyzes of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.

    摘要翻译： 本发明提供了一种隔离单个靶细胞的方法和装置。 该装置包括主体结构，其包括主通道，收集通道和在第一流体结处流体耦合的废物通道。 多个捕获通道与收集通道相交，每个捕获通道在与捕集通道与收集通道的交叉点相邻的位置处的直径小于单个靶细胞的直径。 该装置还包括：成像系统，被配置为对主通道内的各个目标单元和非目标单元进行成像，从而产生成像数据; 处理器，被配置为执行所述成像数据的实时，多变量分析; 以及被配置为引导各个目标细胞的引导系统。 压力源与收集通道，废物通道，第一侧通道和第二侧通道中的一个或多个流体连通。

    HIGH-THROUGHPUT SINGLE-CELL IMAGING, SORTING, AND ISOLATION
    5.
    发明申请
    HIGH-THROUGHPUT SINGLE-CELL IMAGING, SORTING, AND ISOLATION 有权
    高通量单细胞成像，分选和分离

    公开(公告)号：US20140247971A1

    公开(公告)日：2014-09-04

    申请号：US13783767

    申请日：2013-03-04

    申请人： Rajiv Bharadwaj ,  Bahram Fathollahi

    发明人： Rajiv Bharadwaj ,  Bahram Fathollahi

    IPC分类号： G06T7/00

    CPC分类号： H04N5/2251 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  C12M47/04 ,  C12N5/0081 ,  C12N5/0087 ,  G02B21/365 ,  G06T7/0012 ,  H04N5/2256 ,  H04N7/18

    摘要： The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.

    摘要翻译： 本发明提供了一种隔离单个靶细胞的方法和装置。 该装置包括主体结构，其包括主通道，收集通道和在第一流体结处流体耦合的废物通道。 多个捕获通道与收集通道相交，每个捕获通道在与捕集通道与收集通道的交叉点相邻的位置处的直径小于单个靶细胞的直径。 该装置还包括：成像系统，被配置为对主通道内的各个目标单元和非目标单元进行成像，从而产生成像数据; 处理器，被配置为执行所述成像数据的实时，多变量分析; 以及被配置为引导各个目标细胞的引导系统。 压力源与收集通道，废物通道，第一侧通道和第二侧通道中的一个或多个流体连通。

    Electrophoretic sample analysis and approach therefor
    6.
    发明授权
    Electrophoretic sample analysis and approach therefor 有权
    电泳样品分析及方法

    公开(公告)号：US08414754B1

    公开(公告)日：2013-04-09

    申请号：US11755449

    申请日：2007-05-30

    申请人： Juan G. Santiago ,  Byoungsok Jung ,  Rajiv Bharadwaj ,  Tarun Kumar Khurana

    发明人： Juan G. Santiago ,  Byoungsok Jung ,  Rajiv Bharadwaj ,  Tarun Kumar Khurana

    IPC分类号： B01D57/02 ,  G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44769

    摘要： Analysis of samples is facilitated. According to an example embodiment, an electrophoresis approach involves electrophoretically stacking and/or separating a sample or samples. An electrolyte and a mixture of one or more samples with another electrolyte are added to a microchannel or capillary. An electric field is applied to stack (and, in some applications, further separate) the one or more samples. Generally, the electric field and electrolyte are used to facilitate isotachophoretic (ITP) stacking. In some embodiments, a further electric field is applied and used with the electrolyte to facilitate subsequent capillary electrophoresis (CE).

    摘要翻译： 样品分析方便。 根据示例性实施方案，电泳方法涉及电泳堆叠和/或分离样品或样品。 将电解质和一种或多种样品与另一种电解质的混合物加入到微通道或毛细管中。 将一个或多个样品施加到堆叠（并且在一些应用中进一步分离）的电场。 通常，电场和电解质用于促进异羟基磷灰石（ITP）堆叠。 在一些实施方案中，施加另外的电场并与电解质一起使用以促进随后的毛细管电泳（CE）。

    HIGH-THROUGHPUT SINGLE-CELL IMAGING, SORTING, AND ISOLATION
    9.
    发明申请
    HIGH-THROUGHPUT SINGLE-CELL IMAGING, SORTING, AND ISOLATION 审中-公开
    高通量单细胞成像，分选和分离

    公开(公告)号：US20150193928A1

    公开(公告)日：2015-07-09

    申请号：US14595693

    申请日：2015-01-13

    申请人： Rajiv Bharadwaj ,  Bahram Fathollahi

    发明人： Rajiv Bharadwaj ,  Bahram Fathollahi

    IPC分类号： G06T7/00 ,  H04N7/18

    CPC分类号： H04N5/2251 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  C12M47/04 ,  C12N5/0081 ,  C12N5/0087 ,  G02B21/365 ,  G06T7/0012 ,  H04N5/2256 ,  H04N7/18

    摘要： The invention provides a method and apparatus for isolating individual target cells. The apparatus includes a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction. A plurality of trapping channels intersect the collection channel, each trapping channel having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell. The apparatus also includes an imaging system configured to image individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; and a directing system configured to direct the individual target cells. A pressure source is in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.

    摘要翻译： 本发明提供了一种隔离单个靶细胞的方法和装置。 该装置包括主体结构，其包括主通道，收集通道和在第一流体结处流体耦合的废物通道。 多个捕获通道与收集通道相交，每个捕获通道在与捕集通道与收集通道的交叉点相邻的位置处的直径小于单个靶细胞的直径。 该装置还包括：成像系统，被配置为对主通道内的各个目标单元和非目标单元进行成像，从而产生成像数据; 处理器，被配置为执行所述成像数据的实时，多变量分析; 以及被配置为引导各个目标细胞的引导系统。 压力源与收集通道，废物通道，第一侧通道和第二侧通道中的一个或多个流体连通。