公开(公告)号：US20240377297A1

公开(公告)日：2024-11-14

申请号：US18784392

申请日：2024-07-25

Applicant: 10x Genomics, Inc.

Inventor： David Maurice Cox ,  Layla Katiraee

IPC: G01N1/36 ,  B01L9/00

Abstract: A substrate holder includes a base configured to receive a substrate; a cover configured to mateably engage with the base, the cover defining an opening formed by inner sidewalls; and a removable insert defining a surface, the removable insert being configured to be received within the opening of the cover. The removable insert includes a gasket; a projection coupled to the gasket; and at least two insert tabs extending from opposite sides of the removable insert, each insert tab being configured to engage with at least one of the inner sidewalls forming the opening of the cover.

公开(公告)号：US20240035937A1

公开(公告)日：2024-02-01

申请号：US18357610

申请日：2023-07-24

Applicant: 10x Genomics, Inc.

Inventor： David Maurice Cox ,  Layla Katiraee

IPC: G01N1/36 ,  B01L9/00

CPC classification number: G01N1/36 ,  B01L9/527 ,  G01N2001/368 ,  B01L2300/0819 ,  B01L2200/0689 ,  B01L2300/045 ,  B01L2200/025 ,  B01L2300/043

Abstract: A substrate holder includes a base configured to receive a substrate; a cover configured to mateably engage with the base, the cover defining an opening formed by inner sidewalls; and a removable insert defining a surface, the removable insert being configured to be received within the opening of the cover. The removable insert includes a gasket; a projection coupled to the gasket; and at least two insert tabs extending from opposite sides of the removable insert, each insert tab being configured to engage with at least one of the inner sidewalls forming the opening of the cover.

公开(公告)号：US20230340576A1

公开(公告)日：2023-10-26

申请号：US18302421

申请日：2023-04-18

Applicant: 10x Genomics, Inc.

Inventor： Layla Katiraee ,  Michael Schnall-Levin ,  Christina Galonska ,  Marlon Stoeckius

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/154

Abstract: Provided herein are methods of identifying a methylation status of an analyte in a biological sample. Also provided herein are methods that combine identifying the methylation status with spatial technology to identify the location of a methylation status in a biological sample.

公开(公告)号：US20230295699A1

公开(公告)日：2023-09-21

申请号：US18311605

申请日：2023-05-03

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US20230160008A1

公开(公告)日：2023-05-25

申请号：US18100133

申请日：2023-01-23

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

CPC classification number: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US11505828B2

公开(公告)日：2022-11-22

申请号：US17220534

申请日：2021-04-01

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Layla Katiraee

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20220282329A1

公开(公告)日：2022-09-08

申请号：US17707189

申请日：2022-03-29

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20240417784A1

公开(公告)日：2024-12-19

申请号：US18815241

申请日：2024-08-26

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US12071655B2

公开(公告)日：2024-08-27

申请号：US18311605

申请日：2023-05-03

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hanyoup Kim ,  Jerald Sapida ,  Spontaneous Rose McKnight Russell ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12Q1/6841 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065

Abstract: Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

公开(公告)号：US20230279474A1

公开(公告)日：2023-09-07

申请号：US18009093

申请日：2021-06-09

Applicant: 10x Genomics, Inc.

Inventor： Layla Katiraee

IPC: C12Q1/6832 ,  C12Q1/6841

CPC classification number: C12Q1/6832 ,  C12Q1/6841

Abstract: Provided herein are methods for blocking undesirable nucleic acids from binding to capture probes using blocker oligonucleotides.