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    HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS
    1.
    发明申请
    HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS 审中-公开
    高渗透基因组装在DROPLETS

    公开(公告)号：US20150361423A1

    公开(公告)日：2015-12-17

    申请号：US14486346

    申请日：2014-09-15

    Applicant: Agilent Technologies, Inc.

    Inventor： Jeffrey R. Sampson ,  Nicholas M. Sampas

    IPC: C12N15/10

    CPC classification number: C12N15/1093 ,  C12N15/1075

    Abstract: Provided herein is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal indexer sequence and can be assembled to produce a synthon; and (b) hybridizing the oligonucleotide mixture to an array, thereby spatially-separating the different sets of oligonucleotides from one another. In some embodiments the method may comprise (c) contacting the array with a solution, thereby producing, for each feature bound by the oligonucleotides, a discrete droplet comprising the feature and, optionally, placing an immiscible liquid over the droplets, thereby producing, for each feature bound by the oligonucleotides, a discrete reaction chamber defined by a droplet. The method may further comprise incubating the array under conditions by which a synthon is assembled in each of the reaction chambers. Other embodiments are also provided.

    Abstract translation: 本文提供的方法包括：（a）获得多组寡核苷酸的混合物，其中每组中的寡核苷酸各自包含末端分子序列，并且可以组装以产生合成子; 和（b）将寡核苷酸混合物与阵列杂交，从而将不同组的寡核苷酸彼此空间分离。 在一些实施方案中，该方法可以包括（c）使阵列与溶液接触，从而为由寡核苷酸结合的每个特征产生包含特征的离散液滴，并且任选地将不混溶液体放置在液滴上，从而产生 由寡核苷酸结合的每个特征，由液滴限定的离散反应室。 该方法还可以包括在每个反应室中组合合成子的条件下孵育该阵列。 还提供了其他实施例。

    METHOD FOR PRODUCING A POPULATION OF OLIGONUCLEOTIDES THAT HAS REDUCED SYNTHESIS ERRORS
    2.
    发明申请
    METHOD FOR PRODUCING A POPULATION OF OLIGONUCLEOTIDES THAT HAS REDUCED SYNTHESIS ERRORS 审中-公开
    生产减少合成错误的寡核苷酸人群的方法

    公开(公告)号：US20150010953A1

    公开(公告)日：2015-01-08

    申请号：US13935201

    申请日：2013-07-03

    Applicant: AGILENT TECHNOLOGIES, INC.

    Inventor： Derek Lee Lindstrom ,  Jeffrey R. Sampson ,  Daniel E. Ryan

    IPC: C12N15/10 ,  C12P19/34

    CPC classification number: C12N15/1006 ,  C12N15/1003 ,  C12P19/34 ,  C12Q2521/514 ,  C12Q2525/301

    Abstract: Provided herein is a method for producing a population of oligonucleotides that has reduced synthesis errors. In certain embodiments, the method comprises: a) obtaining an initial population of hairpin oligonucleotide molecules that each comprise a double-stranded stem region and a loop region; b) contacting the double-stranded region of the hairpin oligonucleotide molecules with a mismatch binding protein; and c) eliminating any molecules that bind to the mismatch binding protein, thereby producing a population of oligonucleotides that has reduced synthesis errors. A kit and a composition for performing the method are also provided.

    Abstract translation: 本文提供了一种减少合成错误的寡核苷酸群体的制备方法。 在某些实施方案中，所述方法包括：a）获得每个包含双链茎区和环区的发夹寡核苷酸分子的初始群; b）使发夹寡核苷酸分子的双链区域与错配结合蛋白接触; 和c）消除与错配结合蛋白结合的任何分子，从而产生具有降低的合成错误的寡核苷酸群。 还提供了用于执行该方法的试剂盒和组合物。

    HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS
    9.
    发明申请
    HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS 审中-公开
    高渗透基因组装在DROPLETS

    公开(公告)号：US20150361422A1

    公开(公告)日：2015-12-17

    申请号：US14550713

    申请日：2014-11-21

    Applicant: Agilent Technologies, Inc.

    Inventor： Jeffrey R. Sampson ,  Nicholas M. Sampas ,  Joel Myerson ,  Paige Anderson ,  Bo Curry

    IPC: C12N15/10 ,  B01J19/00

    Abstract: Provided herein, among other things, is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal indexer sequence can be assembled to produce a synthon; and (b) hybridizing the oligonucleotide mixture to an array, thereby spatially-separating the different sets of oligonucleotides from one another. Other embodiments are also provided.

    Abstract translation: 本文提供了一种方法，其包括：（a）获得多组寡核苷酸的混合物，其中每组中的寡核苷酸各自包含末端分子序列可以组装以产生合成子; 和（b）将寡核苷酸混合物与阵列杂交，从而将不同组的寡核苷酸彼此空间分离。 还提供了其他实施例。

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