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    Microorganism having the improved gene for hydrogen generation capability, and process for producing hydrogen using the same
    1.
    发明授权
    Microorganism having the improved gene for hydrogen generation capability, and process for producing hydrogen using the same 有权
    具有改善的氢生成能力的基因的微生物以及使用其生产氢的方法

    公开(公告)号：US08728791B2

    公开(公告)日：2014-05-20

    申请号：US11791731

    申请日：2005-12-07

    申请人： Hideaki Yukawa ,  Masayuki Inui ,  Akihito Yoshida ,  Naoto Torata

    发明人： Hideaki Yukawa ,  Masayuki Inui ,  Akihito Yoshida ,  Naoto Torata

    IPC分类号： C12N9/88 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/00 ,  C12Q1/68 ,  C12P3/00 ,  C07H21/04

    CPC分类号： C12N9/0095 ,  C12N9/0008 ,  C12P3/00 ,  C12Y112/07002

    摘要： The present invention provides a microorganism which possesses the formate dehydrogenase gene and hydrogenase gene and contains an exogenous transcription activator gene for formate hydrogen lyase system, characterized in that said microorganism shows the transcription activator for formate hydrogen lyase system highly expressed therein and shows an improved function to generate hydrogen from formic acid, and a process for producing hydrogen using the microorganism.Utilization of the microorganism of the present invention enables the hydrogen production from an organic substrate to be accomplished on a practical, commercial scale. The hydrogen to be produced by the present invention, which is free of carbon monoxide being causative to poisoning of the electrode catalyst for fuel cells, is useful as a fuel for fuel cells.

    摘要翻译： 本发明提供一种具有甲酸脱氢酶基因和氢化酶基因并含有甲酸氢裂解酶系统的外源转录激活子基因的微生物，其特征在于所述微生物显示出在其中高度表达的甲酸氢裂解酶系统的转录激活剂，并显示出改善的功能 从甲酸产生氢气，以及使用微生物生产氢气的方法。 本发明的微生物的利用使得能够以实用的商业规模实现有机底物的氢生成。 本发明生产的不含作为燃料电池用电极催化剂中毒的一氧化碳的氢可用作燃料电池的燃料。

    Method and device for producing hydrogen
    3.
    发明授权
    Method and device for producing hydrogen 有权
    生产氢的方法和装置

    公开(公告)号：US08846358B2

    公开(公告)日：2014-09-30

    申请号：US12435705

    申请日：2009-05-05

    申请人： Akihito Yoshida ,  Hideaki Yukawa ,  Masayuki Inui

    发明人： Akihito Yoshida ,  Hideaki Yukawa ,  Masayuki Inui

    IPC分类号： C12P3/00 ,  C12N9/04 ,  C12N1/00 ,  C12M1/107 ,  C12M1/00

    CPC分类号： C12P3/00 ,  C12M21/04 ,  C12M43/08 ,  C12N9/0006 ,  C12Y101/05006

    摘要： In a step of contacting an organic material including formic acid ions and a carbon source other than the formic acid ions with a microorganism having a formate dehydrogenase gene and a hydrogenase gene under an anaerobic condition, concentration of the formic acid ions in the organic material is set to be not less than 0.01 mol/L and not more than 0.5 mol/L, and concentration of the carbon source is set to not less than 0.1 mmol/L and not more than 200 mmol/L. This allows continuously producing hydrogen for a long time, without dropping the ability of the microorganism to produce hydrogen.

    摘要翻译： 在含有甲酸离子和除甲酸离子以外的碳源的有机材料与厌氧条件下具有甲酸脱氢酶基因和氢化酶基因的微生物接触的步骤中，有机材料中的甲酸离子的浓度为 设定为0.01mol / L以上且0.5mol / L以下，碳源的浓度为0.1mmol / L以上200mmol / L以下。 这允许长时间连续生产氢气，而不降低微生物产生氢气的能力。

    METHOD AND DEVICE FOR PRODUCING HYDROGEN
    5.
    发明申请
    METHOD AND DEVICE FOR PRODUCING HYDROGEN 有权
    用于生产氢的方法和装置

    公开(公告)号：US20090280548A1

    公开(公告)日：2009-11-12

    申请号：US12435705

    申请日：2009-05-05

    申请人： Akihito YOSHIDA ,  Hideaki Yukawa ,  Masayuki Inui

    发明人： Akihito YOSHIDA ,  Hideaki Yukawa ,  Masayuki Inui

    IPC分类号： C12P3/00 ,  C12M3/00

    CPC分类号： C12P3/00 ,  C12M21/04 ,  C12M43/08 ,  C12N9/0006 ,  C12Y101/05006

    摘要： In a step of contacting an organic material including formic acid ions and a carbon source other than the formic acid ions with a microorganism having a formate dehydrogenase gene and a hydrogenase gene under an anaerobic condition, concentration of the formic acid ions in the organic material is set to be not less than 0.01 mol/L and not more than 0.5 mol/L, and concentration of the carbon source is set to not less than 0.1 mmol/L and not more than 200 mmol/L. This allows continuously producing hydrogen for a long time, without dropping the ability of the microorganism to produce hydrogen.

    摘要翻译： 在含有甲酸离子和除甲酸离子以外的碳源的有机材料与厌氧条件下具有甲酸脱氢酶基因和氢化酶基因的微生物接触的步骤中，有机材料中的甲酸离子的浓度为 设定为0.01mol / L以上且0.5mol / L以下，碳源的浓度为0.1mmol / L以上200mmol / L以下。 这允许长时间连续生产氢气，而不降低微生物产生氢气的能力。

    Microorganism having the Improved Gene for Hydrogen Generation Capability, and Process for Producing Hydrogen Using the Same
    6.
    发明申请
    Microorganism having the Improved Gene for Hydrogen Generation Capability, and Process for Producing Hydrogen Using the Same 有权
    具有改进的氢生成能力基因的微生物以及使用其的氢生产方法

    公开(公告)号：US20090170176A1

    公开(公告)日：2009-07-02

    申请号：US11791731

    申请日：2005-12-07

    申请人： Hideaki Yukawa ,  Masayuki Inui ,  Akihito Yoshida ,  Naoto Torata

    发明人： Hideaki Yukawa ,  Masayuki Inui ,  Akihito Yoshida ,  Naoto Torata

    IPC分类号： C12P3/00 ,  C12N1/21

    CPC分类号： C12N9/0095 ,  C12N9/0008 ,  C12P3/00 ,  C12Y112/07002

    摘要： The present invention provides a microorganism which possesses the formate dehydrogenase gene and hydrogenase gene and contains an exogenous transcription activator gene for formate hydrogen lyase system, characterized in that said microorganism shows the transcription activator for formate hydrogen lyase system highly expressed therein and shows an improved function to generate hydrogen from formic acid, and a process for producing hydrogen using the microorganism.Utilization of the microorganism of the present invention enables the hydrogen production from an organic substrate to be accomplished on a practical, commercial scale. The hydrogen to be produced by the present invention, which is free of carbon monoxide being causative to poisoning of the electrode catalyst for fuel cells, is useful as a fuel for fuel cells.

    摘要翻译： 本发明提供一种具有甲酸脱氢酶基因和氢化酶基因并含有甲酸氢裂解酶系统的外源转录激活子基因的微生物，其特征在于所述微生物显示出在其中高度表达的甲酸氢裂解酶系统的转录激活剂，并显示出改善的功能 从甲酸产生氢气，以及使用微生物生产氢气的方法。 本发明的微生物的利用使得能够以实用的商业规模实现有机底物的氢生产。 本发明生产的不含作为燃料电池用电极催化剂中毒的一氧化碳的氢气可用作燃料电池用燃料。

    CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING PHENOL USING THE SAME
    7.
    发明申请
    CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING PHENOL USING THE SAME 有权
    CORYNEFORM BACTERIUM变压器和使用它的苯酚生产方法

    公开(公告)号：US20130266999A1

    公开(公告)日：2013-10-10

    申请号：US13884536

    申请日：2011-11-09

    申请人： Hideaki Yukawa ,  Masayuki Inui

    发明人： Hideaki Yukawa ,  Masayuki Inui

    IPC分类号： C12N15/77 ,  C12P7/22

    CPC分类号： C12N15/77 ,  C12N9/88 ,  C12P7/22 ,  C12Y401/01061

    摘要： Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into Corynebacterium glutamicum as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing 4-hydroxybenzoate or a salt thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.

    摘要翻译： 提供一种通过将编码具有4-羟基苯甲酸脱羧酶活性的酶的基因转移到谷氨酸棒杆菌中作为宿主构建的产生苯酚的转化体。 还提供了一种生产苯酚的方法，其包括在还原条件下使转化体在含有4-羟基苯甲酸酯或其盐的反应混合物中反应的步骤和从反应混合物中收集苯酚的步骤。

    CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING PHENOL USING THE SAME
    8.
    发明申请
    CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING PHENOL USING THE SAME 有权
    CORYNEFORM BACTERIUM变压器和使用它的苯酚生产方法

    公开(公告)号：US20130203139A1

    公开(公告)日：2013-08-08

    申请号：US13821212

    申请日：2011-09-07

    申请人： Hideaki Yukawa ,  Masayuki Inui

    发明人： Hideaki Yukawa ,  Masayuki Inui

    IPC分类号： C12P7/22

    CPC分类号： C12P7/22 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12Y401/99002 ,  Y02P20/52

    摘要： A phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into Corynebacterium glutamicum as a host can efficiently produce phenol from a saccharide. Specifically, preferred is a process which comprises a step of reacting the transformant in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.

    摘要翻译： 通过将编码具有酪氨酸酚裂解酶活性的酶的基因转移到谷氨酸棒杆菌中作为宿主构建的产生苯酚的转化体可以有效地从糖中产生苯酚。 具体而言，优选包括使还原条件下含有糖的反应混合物中的转化体反应的工序和从反应混合物中收集苯酚的工序。

    Coryneform bacterium transformant and process for producing phenol using the same
    10.
    发明授权
    Coryneform bacterium transformant and process for producing phenol using the same 有权
    棒状细菌转化体及使用其制备苯酚的方法

    公开(公告)号：US09404115B2

    公开(公告)日：2016-08-02

    申请号：US13884549

    申请日：2011-11-09

    申请人： Hideaki Yukawa ,  Masayuki Inui

    发明人： Hideaki Yukawa ,  Masayuki Inui

    IPC分类号： C12N1/21 ,  C12P7/22 ,  C12N9/88 ,  C12N15/77

    CPC分类号： C12N15/77 ,  C12N9/88 ,  C12P7/22 ,  Y02P20/52

    摘要： Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having chorismate-pyruvate lyase activity and a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.

    摘要翻译： 本发明提供一种生产苯酚的转化体，其通过将编码具有4-硝基苯甲酸脱羧酶活性的酶的基因编码为具有佐罗沙普 - 丙酮酸裂合酶活性的酶的基因转移到作为宿主的棒状细菌中构建。 还提供了一种生产苯酚的方法，其包括使得转化体在还原条件下在含有糖的反应混合物中反应的步骤和从反应混合物中收集苯酚的步骤。