公开(公告)号：US10273485B2

公开(公告)日：2019-04-30

申请号：US15116600

申请日：2015-02-06

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker

IPC: C12N1/20 ,  C12N15/52 ,  C12N1/02 ,  C12N9/12 ,  C12P7/46 ,  C12N9/04 ,  C12N9/10 ,  C12N15/74

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.

公开(公告)号：US09850506B2

公开(公告)日：2017-12-26

申请号：US15116896

申请日：2015-05-02

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C07K14/285 ,  C12N1/20 ,  C12N15/52

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the fruA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

公开(公告)号：US20140127764A1

公开(公告)日：2014-05-08

申请号：US14150206

申请日：2014-01-08

Applicant: BASF SE

Inventor： Hartwig Schröder ,  Stefan Haefner ,  Gregory Von Abendroth ,  Rajan Hollmann ,  Aline Raddatz ,  Hansgeorg Ernst ,  Hans Gurski

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N1/32 ,  C12N9/1029 ,  C12R1/01 ,  C12Y203/01054

Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

Abstract translation: 本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及 涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。 本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。

公开(公告)号：US10287558B2

公开(公告)日：2019-05-14

申请号：US15116669

申请日：2015-02-02

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker ,  Anna Lange ,  Benjamin J. Lyons ,  Thomas J. Lyons ,  Eudes De Crecy ,  Ewa Hughes

IPC: C12N9/12 ,  C12P7/46 ,  C12N15/52

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the pykA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

公开(公告)号：US09932612B2

公开(公告)日：2018-04-03

申请号：US14676317

申请日：2015-04-01

Applicant: BASF SE

Inventor： Hartwig Schröder ,  Stefan Haefner ,  Gregory Von Abendroth ,  Rajan Hollmann ,  Aline Raddatz ,  Hansgeorg Ernst ,  Hans Gurski

IPC: C12P7/46 ,  C12N1/32 ,  C12N9/10 ,  C12R1/01

CPC classification number: C12P7/46 ,  C12N1/32 ,  C12N9/1029 ,  C12R1/01 ,  C12Y203/01054

Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

公开(公告)号：US20170166937A1

公开(公告)日：2017-06-15

申请号：US15116896

申请日：2015-05-02

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C07K14/285 ,  C12N1/20 ,  C12N15/52

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the fruA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

公开(公告)号：US10513693B2

公开(公告)日：2019-12-24

申请号：US15127181

申请日：2015-03-18

Applicant: BASF SE

Inventor： Esther Dantas Costa ,  Oskar Zelder ,  Hartwig Schröder ,  Stefan Haefner ,  Joanna Martyna Krawczyk ,  Gregory Von Abendroth ,  Christian Riedele ,  Torsten Renz

IPC: C12N9/04 ,  C12N9/10 ,  C12P7/46 ,  C07K14/285

Abstract: The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).

公开(公告)号：US20180030418A1

公开(公告)日：2018-02-01

申请号：US15127181

申请日：2015-03-18

Applicant: BASF SE

Inventor： Esther Dantas Costa ,  Oskar Zelder ,  Hartwig Schröder ,  Stefan Haefner ,  Joanna Martyna Krawczyk ,  Gregory Von Abendroth ,  Christian Riedele ,  Torsten Renz

IPC: C12N9/04 ,  C12N9/10

CPC classification number: C12N9/0006 ,  C07K14/285 ,  C12N9/1029 ,  C12P7/46 ,  C12Y101/01027 ,  C12Y203/01054

Abstract: The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).

公开(公告)号：US20160362696A1

公开(公告)日：2016-12-15

申请号：US15116600

申请日：2015-02-06

Applicant: BASF SE

Inventor： Joanna Martyna Krawczyk ,  Stefan Haefner ,  Hartwig Schröder ,  Esther Dantas Costa ,  Oskar Zelder ,  Gregory Von Abendroth ,  Christoph Wittmann ,  René Stellmacher ,  Judith Becker

IPC: C12N15/52 ,  C12N15/74 ,  C12N9/04 ,  C12N9/10 ,  C12P7/46 ,  C12N9/12

CPC classification number: C12N15/52 ,  C12N1/02 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1288 ,  C12N15/74 ,  C12P7/46 ,  C12Y101/01 ,  C12Y203/01054 ,  C12Y207/08031

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the wcaJ-gene. The present invention also relates to a method for producing an organic compound and to the use of a modified microorganism.

Abstract translation: 本发明涉及与其野生型相比具有由wcaJ基因编码的酶的活性降低的修饰微生物。 本发明还涉及有机化合物的制造方法和改性微生物的用途。

公开(公告)号：US20150197776A1

公开(公告)日：2015-07-16

申请号：US14676317

申请日：2015-04-01

Applicant: BASF SE

Inventor： Hartwig Schröder ,  Stefan Haefner ,  Gregory Von Abendroth ,  Rajan Hollmann ,  Aline Raddatz ,  Hansgeorg Ernst ,  Hans Gurski

IPC: C12P7/46

CPC classification number: C12P7/46 ,  C12N1/32 ,  C12N9/1029 ,  C12R1/01 ,  C12Y203/01054

Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

Abstract translation: 本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及 涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。 本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。