公开(公告)号：US20140370569A1

公开(公告)日：2014-12-18

申请号：US14374556

申请日：2013-01-29

Applicant: BASF SE

Inventor： Tobias Kueppers ,  Victoria Steffen ,  Renee Charlott Eichstadt ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts ,  Hendrik Hellmuth ,  Thomas Weber ,  Timothy O'Connell

IPC: C12N9/26 ,  C12N9/50

CPC classification number: C12N9/2411 ,  C12N9/00 ,  C12N9/2414 ,  C12N9/50 ,  C12N9/54 ,  C12N15/75 ,  C12P21/02

Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.

Abstract translation: 在微生物发酵中，目的是提高蛋白质的产率。 这是通过一种方法实现的，其中将表达构建体引入到包含启动子和编码该蛋白质的核酸的物种短小芽孢杆菌的微生物中，并且所述蛋白质在所述表达构建体中表达。

公开(公告)号：US20200095564A1

公开(公告)日：2020-03-26

申请号：US16700322

申请日：2019-12-02

Applicant: BASF SE

Inventor： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maure ,  Johannes Bongaerts

IPC: C12N9/50 ,  C12N15/75 ,  C12N15/70 ,  C12N15/62

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

公开(公告)号：US20180073005A1

公开(公告)日：2018-03-15

申请号：US15712652

申请日：2017-09-22

Applicant: BASF SE

Inventor： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

IPC: C12N9/50 ,  C12N15/62 ,  C12N15/70 ,  C12N15/75

CPC classification number: C12N9/50 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

公开(公告)号：US09725704B2

公开(公告)日：2017-08-08

申请号：US14374556

申请日：2013-01-29

Applicant: BASF SE

Inventor： Tobias Kueppers ,  Victoria Steffen ,  Renee Charlott Eichstadt ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts ,  Hendrik Hellmuth ,  Thomas Weber ,  Timothy O'Connell

IPC: C12N9/26 ,  C12N9/50 ,  C12N1/20 ,  C12N15/74 ,  C12P21/06 ,  C12P19/34 ,  C12N15/75 ,  C12P21/02 ,  C12N9/00 ,  C12N9/54

CPC classification number: C12N9/2411 ,  C12N9/00 ,  C12N9/2414 ,  C12N9/50 ,  C12N9/54 ,  C12N15/75 ,  C12P21/02

Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.

公开(公告)号：US20170067064A1

公开(公告)日：2017-03-09

申请号：US15182839

申请日：2016-06-15

Applicant: BASF SE

Inventor： Cornelius Bessler ,  Jorg Feesche ,  Stefan Evers ,  Karl-Heinz Maurer ,  Armin Ehrenreich ,  Birgit Veith ,  Heiko Liesegang ,  Anke Henne ,  Christina Herzberg ,  Gerhard Gottschalk

IPC: C12N15/52 ,  C12P7/42 ,  C12P7/52 ,  C12P13/00 ,  C12N15/75 ,  C12N9/84 ,  C12N9/88 ,  C12N9/04 ,  C12N9/02 ,  C12N9/00 ,  C12N9/12 ,  C12P7/16 ,  C12N9/10

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/16 ,  C12N9/78 ,  C12N9/80 ,  C12N9/84 ,  C12N9/88 ,  C12N9/93 ,  C12N15/75 ,  C12P7/16 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P13/001 ,  C12Y101/01 ,  C12Y101/01035 ,  C12Y101/01157 ,  C12Y102/04002 ,  C12Y103/99 ,  C12Y203/01019 ,  C12Y206/01042 ,  C12Y207/02007 ,  C12Y305/01001 ,  C12Y305/01011 ,  C12Y401/01018 ,  C12Y401/01019 ,  C12Y402/01017 ,  C12Y402/01055 ,  C12Y602/01 ,  C12Y602/01001 ,  Y02E50/10 ,  Y02P20/52

Abstract: The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived thereform and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesised via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

Abstract translation: 本发明涉及地衣芽孢杆菌的25个迄今未描述的基因以及衍生自地衣芽孢杆菌的基因产物及其全部充分同源的核酸及其蛋白质。 它们发生在五种不同的代谢途径中，用于形成气味物质。 所讨论的代谢途径是用于合成：1）异戊酸（作为亮氨酸分解代谢的一部分），2）2-甲基丁酸和/或异丁酸（作为缬氨酸和/或异亮氨酸分解代谢的一部分） 3）丁醇和/或丁酸（作为丁酸代谢的一部分），4）丙酸（作为丙酸代谢的一部分）和/或5）尸胺和/或腐胺（作为赖氨酸分解代谢的一部分） 和/或精氨酸）。 这些基因的鉴定允许开发生物技术生产方法，其被改进到对于辅助这些核酸的程度，可以通过使微生物中相应的基因失活来减少通过这些代谢途径合成的恶臭物质的形成 用于生物技术生产。 此外，这些基因产物因此可用于制备反应或根据其各自生物化学性质的方法。

公开(公告)号：US11046961B2

公开(公告)日：2021-06-29

申请号：US16700322

申请日：2019-12-02

Applicant: BASF SE

Inventor： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

IPC: C12N9/50 ,  C12N15/62 ,  C12N15/70 ,  C12N15/75

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.