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公开(公告)号：US20240093211A1
公开(公告)日：2024-03-21
申请号：US18463923
申请日：2023-09-08
Applicant: BASF SE
Inventor： Max Fabian Felle , Stefan Jenewein , Kerstin Hage , Holger Hartmann
CPC classification number: C12N15/75 , C12N9/0065 , C12N15/63 , C12P1/04 , C12Y111/01006
Abstract: The present invention generally relates to the field of fermentation technology and microorganisms useful for such fermentations. The invention also relates to materials including nucleic acids and proteins useful for altering fermentation characteristics of microorganisms, and to microorganisms comprising such nucleic acids and/or proteins.
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公开(公告)号：US20230272358A1
公开(公告)日：2023-08-31
申请号：US18017426
申请日：2021-07-23
Applicant: BASF SE
Inventor： Stefan Jenewein , Max Fabian Felle , Christopher Sauer , Tobias Klein
CPC classification number: C12N9/48 , C12N15/75 , C12N9/90 , C12N1/20 , C12R2001/10
Abstract: The present invention relates to a Bacillus host cell belonging to the species Bacillus lichemformis or Bacillus pumilus in which the chromosomal alr gene has been inactivated. Said bacterial host cell comprises a plasmid comprising at least one autonomous replication sequence, a first polynucleotide encoding at least one polypeptide of interest, wherein said first polynucleotide is operably linked to a promoter, and a second polynucleotide encoding an alanine racemase which is not native to the host cell, wherein said second polynucleotide is operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.
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公开(公告)号：US20190112612A1
公开(公告)日：2019-04-18
申请号：US16062291
申请日：2016-12-08
Applicant: BASF SE
Inventor： Max Fabian Felle , Stefan Jenewein , Kerstin Hage , Holger Hartmann
Abstract: The present invention generally relates to the field of fermentation technology and microorganisms useful for such fermentations. The invention also relates to materials including nucleic acids and proteins useful for altering fermentation characteristics of microorganisms, and to microorganisms comprising such nucleic acids and/or proteins.
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公开(公告)号：US20250051822A1
公开(公告)日：2025-02-13
申请号：US18720123
申请日：2022-12-19
Applicant: BASF SE
Inventor： Max Fabian Felle , Christopher Sauer , Mathis Appelbaum , Stefan Jenewein , Sebastian Schoof , Christian Riedele
Abstract: The present invention relates to a method for producing at least one polypeptide of interest in a Bacillus host cell, said method comprising the steps of a) providing a Bacillus host cell comprising a polynucleotide encoding the at least one polypeptide of interest, b) cultivating the Bacillus host cell under conditions which allow for intracellular accumulation of said at least one polypeptide of interest, thereby producing said at least one polypeptide of interest, c) stop of aeration and addition of feed, and d) incubating the cells for at least hours. Moreover, the present invention relates to a Bacillus host cell comprising a polynucleotide encoding at least one polypeptide of interest lacking a functional secretion sequence, wherein the ispA gene has been inactivated in the Bacillus host cell.
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公开(公告)号：US20220186177A1
公开(公告)日：2022-06-16
申请号：US17432204
申请日：2020-02-18
Applicant: BASF SE
Inventor： Andreas Daub , Aydin Golabgir Anbarani , Tobias Klein , Stephan Freyer , Thomas Kaeding , Max Fabian Felle , Paul Igor Costea
Abstract: The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of inter-est comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and magnesium ions to the fermentation medium.
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Patent Agency Ranking
公开(公告)号：US20200181627A1
公开(公告)日：2020-06-11
申请号：US16631999
申请日：2018-07-11
Applicant: BASF SE
Inventor： Max Fabian Felle , Bogdan Tokovenko
Abstract: The present invention is directed to a nucleic acid construct comprising a polynucleotide operably linked to one or more control sequence that directs the expression of the polynucleotide in a host cell, wherein at least one control sequence comprises a promoter sequence of an operon comprising a secA gene or a functional fragment or functional variant thereof and wherein said promoter sequence is heterologous to the polynucleotide. In a further embodiment, the invention is directed to an expression vector and a host cell comprising the nucleic acid construct comprising the promoter sequence described herein and a method of expressing a polynucleotide in a host cell.
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公开(公告)号：US20230295603A1
公开(公告)日：2023-09-21
申请号：US18017430
申请日：2021-07-23
Applicant: BASF SE
Inventor： Max Fabian Felle , Stefan Jenewein , Christopher Sauer , Tobias Klein
CPC classification number: C12N9/90 , C12N15/75 , C12Y501/01001 , C12N2800/101
Abstract: The present invention relates to a bacterial host cell in which a first chromosomal gene encoding a first alanine racemase and a second chromosomal gene encoding a second alanine racemase have been inactivated. Said bacterial host cell comprises – either on a plasmid comprising at least one autonomous replication sequence or present as multiple copies in the chromosome – a gene expression cassette comprising a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter, and a polynucleotide encoding a third alanine racemase, operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.
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公开(公告)号：US20230227834A1
公开(公告)日：2023-07-20
申请号：US18002055
申请日：2021-06-17
Applicant: BASF SE
Inventor： Max Fabian Felle , Christopher SAUER , Karen STETTER , Diana WOLF , Thorsten MASCHER
CPC classification number: C12N15/69 , C12N15/902 , C12N15/65
Abstract: Described herein are materials and methods for user-controlled adjustment of plasmid copy numbers.
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公开(公告)号：US20180371444A1
公开(公告)日：2018-12-27
申请号：US16062289
申请日：2016-12-08
Applicant: BASF-SE
Inventor： Max Fabian Felle , Stefan Jenewein , Handtke Stefan , Birgit Voigt , Thomas Schweder , Michael Hecker
IPC: C12N9/54 , C12N15/52 , C12N15/113 , C12N9/64
Abstract: The present invention generally relates to the field of fermentation technology and microorganisms useful for such fermentations. The invention also relates to materials including nucleic acids and proteins useful for altering fermentation characteristics of microorganisms, and to microorganisms comprising such nucleic acids and/or proteins. In particular, the invention relates to materials for conferring, modifying or reducing microbial stress resistance.
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公开(公告)号：US20240060110A1
公开(公告)日：2024-02-22
申请号：US18030700
申请日：2021-10-06
Applicant: BASF SE
Inventor： Stefan Jenewein , Max Fabian Felle , Christopher Sauer , Paul Igor Costea
CPC classification number: C12P21/02 , C12Y301/01001 , C12N9/20 , C12Y301/02002 , C12N9/16
Abstract: The present invention provides a modified Bacillus cell for the production of a compound of interest, wherein the Bacillus cell comprises a genetic modification that leads to decreased production of enzymes with lipase and/or esterase activity. The use of compounds of interest produced with said improved Bacillus host cells reduces the formation of malodour and/or off-flavour when used for applications such as washing or cleaning or as feed or food additives.
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