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1.发明申请公开(公告)号：US20190134630A1
公开(公告)日：2019-05-09
申请号：US15989549
申请日：2018-05-25
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00 , G01N33/558
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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2.发明授权公开(公告)号：US10010882B2
公开(公告)日：2018-07-03
申请号：US14520568
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00 , G01N33/558
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0424 , B01L2400/0433 , B01L2400/0454 , G01N33/558 , G01N2469/20
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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公开(公告)号：US11305283B2
公开(公告)日：2022-04-19
申请号：US16509166
申请日：2019-07-11
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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公开(公告)号：US10376886B2
公开(公告)日：2019-08-13
申请号：US15843122
申请日：2017-12-15
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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公开(公告)号：US09889445B2
公开(公告)日：2018-02-13
申请号：US14521447
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M Jimena Loureiro
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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Patent Agency Ranking
公开(公告)号：US20150165436A1
公开(公告)日：2015-06-18
申请号：US14521447
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
Abstract translation: 在微流体装置中保持笔的生物活性可以通过放置在保持笔中捕获物体来测定，所述对象捕获物质与生物活性产生的特定感兴趣物质结合。 然后可以在微流体装置中或在从微流体装置输出捕获物体之后评估与每个捕获物体结合的感兴趣的生物材料。 该评估可用于表征每支持笔中的生物活性。 生物活性可以是生产感兴趣的生物材料。 因此，生物活性可以对应于或来自一个或多个生物细胞。 持有笔内的生物细胞可以是克隆细胞集落。 可以在保持每个菌落的克隆状态的同时测定每个克隆细胞集落的生物学活性。
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7.发明公开公开(公告)号：US20230347347A1
公开(公告)日：2023-11-02
申请号：US18068207
申请日：2022-12-19
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00
CPC classification number: B01L3/502761 , B01L2300/087 , B01L2300/0816 , B01L2200/0647 , B01L2200/0668 , B01L2300/0864 , B01L2400/0424 , B01L2400/0433 , B01L2400/0454 , B01L2200/0652 , G01N2469/20
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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公开(公告)号：US20160318038A1
公开(公告)日：2016-11-03
申请号：US15207210
申请日：2016-07-11
Applicant: Berkeley Lights, Inc.
Inventor： Steven W. Short , Ming C. Wu
CPC classification number: B03C5/005 , B01L3/502761 , B01L2400/0424 , B03C5/026 , B03C2201/26
Abstract: A microfluidic optoelectronic tweezers (OET) device can comprise dielectrophoresis (DEP) electrodes that can be activated and deactivated by controlling a beam of light directed onto photosensitive elements that are disposed in locations that are spaced apart from the DEP electrodes. The photosensitive elements can be photodiodes, which can switch the switch mechanisms that connect the DEP electrodes to a power electrode between an off state and an on state.
Abstract translation: 微流体光电镊子（OET）装置可以包括介电电泳（DEP）电极，其可以通过控制定向到设置在与DEP电极间隔开的位置的感光元件上的光束而被激活和去激活。 感光元件可以是光电二极管，其可以将连接DEP电极的开关机构切换到关闭状态和导通状态之间的功率电极。
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9.发明申请DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus 审中-公开DEP力控制和电喷雾控制在同一微流体装置的不同部分公开(公告)号：US20150306598A1
公开(公告)日：2015-10-29
申请号：US14262140
申请日：2014-04-25
Applicant: Berkeley Lights, Inc.
Inventor： Igor Y. Khandros , J. Tanner Nevill , Steven W. Short , Ming C. Wu
IPC: B01L3/00
CPC classification number: B01L3/56 , B01L3/502761 , B01L3/502784 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0819 , B01L2300/0864 , B01L2400/0424 , B01L2400/0427 , B01L2400/086 , B03C5/005 , B03C5/026 , B03C2201/26 , C12M23/16 , C12M25/00
Abstract: A microfluidic apparatus can comprise a dielectrophoresis (DEP) configured section for holding a first liquid medium and selectively inducing net DEP forces in the first liquid medium. The microfluidic apparatus can also comprise an electrowetting (EW) configured section for holding a second liquid medium on an electrowetting surface and selectively changing a wetting property of the electrowetting surface. The DEP configured section can be utilized to select and move a micro-object in the first liquid medium. The EW configured section can be utilized to pull a droplet of the first liquid medium into the second liquid medium.
Abstract translation: 微流体装置可以包括用于保持第一液体介质并在第一液体介质中选择性地诱导净DEP力的介电电泳（DEP）构造的部分。 微流体装置还可以包括电润湿（EW）构造的部分，用于将第二液体介质保持在电润湿表面上并且选择性地改变电润湿表面的润湿性质。 DEP配置部分可用于选择和移动第一液体介质中的微物体。 EW配置部分可用于将第一液体介质的液滴拉入第二液体介质。
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10.发明申请Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device 审中-公开从微流体装置中的各种生物细胞捕获特定的核酸材料公开(公告)号：US20150166326A1
公开(公告)日：2015-06-18
申请号：US14133361
申请日：2013-12-18
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Eric D. Hobbs , Steven W. Short , Mark P. White
CPC classification number: B03C5/005 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/0424 , B01L2400/0454 , B03C5/026 , B03C2201/26
Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.
Abstract translation: 可以在微流体装置中选择各种生物细胞，并将其移动到装置中的隔离笔中。 然后，细胞可以在笔中裂解，从而释放核酸物质，其可被笔中的一个或多个捕获物捕获。 然后可以从笔中取出捕获的核酸材料的捕获物体。 捕获对象可以包括唯一的标识符，允许每个捕获对象与由对象捕获的核酸材料源自的单个细胞相关联。
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