公开(公告)号：US10022721B2

公开(公告)日：2018-07-17

申请号：US14470860

申请日：2014-08-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sepehr Kiani ,  Joshua Blouwolff ,  Adnan Esmail ,  Jason Hung ,  Tony Hung ,  Adam R. Abate ,  Scott Powers ,  Pascaline Mary

IPC: B01L3/00 ,  C12N15/10 ,  C12Q1/68 ,  G01N27/00 ,  B01L7/00 ,  C12M1/00

Abstract: Methods and systems for manipulating drops in microfluidic channels are provided.

公开(公告)号：US10012592B2

公开(公告)日：2018-07-03

申请号：US15722486

申请日：2017-10-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Abate ,  John Lawrence Emhoff ,  Tony Hung ,  Adnan Moez Esmail ,  Sepehr Kiani ,  Pascaline Mary

IPC: G01J1/10 ,  G01J3/30 ,  G01N21/64 ,  G01N21/76 ,  G01N21/25 ,  C12Q1/6806

CPC classification number: G01N21/6486 ,  C12Q1/6806 ,  G01N21/255 ,  G01N21/76 ,  G01N2201/06113 ,  G01N2201/062 ,  C12Q2545/101 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2563/161 ,  C12Q2565/629

Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.

公开(公告)号：US10215771B2

公开(公告)日：2019-02-26

申请号：US14932537

申请日：2015-11-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Aaron Weber ,  Tony Hung ,  Sepehr Kiani

IPC: G01P5/18 ,  G01P5/20 ,  G01P5/26

Abstract: Methods and systems are provided for measuring a velocity of a droplet passing through a microfluidic channel.

公开(公告)号：US20170128937A1

公开(公告)日：2017-05-11

申请号：US15410913

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tony Hung ,  Adnan Esmail ,  Sepehr Kiani

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502784 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/1861 ,  B01L2400/0415 ,  B01L2400/0448 ,  B01L2400/0487

Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.

公开(公告)号：US10730045B2

公开(公告)日：2020-08-04

申请号：US16196337

申请日：2018-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tony Hung ,  Adnan Esmail ,  Sepehr Kiani

IPC: B01L3/00

Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.

公开(公告)号：US10589274B2

公开(公告)日：2020-03-17

申请号：US15986718

申请日：2018-05-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sepehr Kiani ,  Joshua Blouwolff ,  Adnan Esmail ,  Jason Hung ,  Tony Hung ,  Adam R. Abate ,  Scott Powers ,  Pascaline Mary

IPC: B01L3/00 ,  C12N15/10 ,  B01L7/00

Abstract: Methods and systems for manipulating drops in microfluidic channels are provided.

公开(公告)号：US10569268B2

公开(公告)日：2020-02-25

申请号：US15822742

申请日：2017-11-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Abate ,  Sepehr Kiani ,  Tony Hung ,  Pascaline Mary ,  Adnan Moez Esmail

IPC: B01L3/00 ,  B01F13/00 ,  F04B19/00 ,  F16K99/00

Abstract: The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.

公开(公告)号：US09821312B2

公开(公告)日：2017-11-21

申请号：US14427404

申请日：2013-09-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adnan Esmail ,  John Healy ,  Tony Hung ,  Sepehr Kiani ,  Pascaline Mary ,  Tal Raz

IPC: B01L3/00 ,  G01N35/08 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2300/1861 ,  B01L2400/0415 ,  B01L2400/0433 ,  B01L2400/0448 ,  B01L2400/088 ,  G01N35/08 ,  Y10T436/143333 ,  Y10T436/2575

Abstract: The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

公开(公告)号：US10343167B2

公开(公告)日：2019-07-09

申请号：US15691196

申请日：2017-08-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adnan Esmail ,  Tal Raz ,  John Healy ,  Tony Hung ,  Sepehr Kiani ,  Pascaline Mary

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/08 ,  C12Q1/6869

Abstract: The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

公开(公告)号：US10124342B2

公开(公告)日：2018-11-13

申请号：US15594404

申请日：2017-05-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Christopher Possinger ,  Sepehr Kiani

IPC: B01L7/00 ,  C12P19/34 ,  F25B21/04 ,  F28F13/14 ,  B01L3/00 ,  C12Q1/686

Abstract: The present invention generally pertains to a system which utilizes floating thermal contact to enable PCR, in a thermal management device. Comprised of multiple thermal zones attached to a framework, each zone an individually actuated, isolated sub-assembly. The actuation of each zone ensures physical contact with an uneven, yet flat microfluidic chip to achieve effective, conductive heat transfer. The isolation of each zone serves to insulate the thermal zones from each other and minimize undesired heat transfer between adjacent zones, so that each zone is at a proper, uniform temperature.