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公开(公告)号：US20220250073A1
公开(公告)日：2022-08-11
申请号：US17665482
申请日：2022-02-04
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Glen Krueger , David Vrane , Kuncheng Wang , Qiuta Gu
Abstract: A compact sorting flow cytometer system is disclosed. The system includes a fluidics system having a flow cell and a deflection chamber in communication with the flow cell to receive drops in a stream of a sample biological fluid with one or more biological cells or particles and selectively deflect the drops in the stream of the sample biological fluid with the one or more biological cells or particles; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers. The DDU system includes a case or a housing with an open face surround by edges of the case, the case forming a portion of a containment chamber, the case having a top side opening aligned with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid into one or more containers in the containment chamber, a seal mounted around edges of the case, one or more hinges coupled to a bottom portion of the case, and a door coupled to the one or more hinges to pivot the door about the one or more hinges, the door when closed to press against the seal and close off the containment chamber from an external environment.
A method for evacuation of air in a containment chamber of a flow cytometer is disclosed. The method includes turning off a return fan in a first tunnel between an air conditioning chamber and a containment chamber; turning on an evacuation fan in a second tunnel between the air conditioning chamber and the containment chamber, the evacuation fan pulling air out of the containment chamber into the air conditioning chamber, opening a valve in an evacuation vent, the evacuation fan pushing air out of the air conditioning chamber through the evacuation vent into the environment; and continuously running the evacuation fan for a predetermined period of time to evacuate air out of the containment chamber.-
公开(公告)号：US20220128450A9
公开(公告)日：2022-04-28
申请号：US15942430
申请日：2018-03-30
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase , Wenbin Jiang
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.
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公开(公告)号：US20210262917A1
公开(公告)日：2021-08-26
申请号：US17302651
申请日：2021-05-10
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
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公开(公告)号：US11029243B2
公开(公告)日：2021-06-08
申请号：US15659610
申请日：2017-07-25
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
IPC: G01N15/00 , G01N21/00 , G01J3/00 , G01N15/14 , G01J1/08 , G01N21/47 , G01J3/02 , G01J3/36 , G01N15/10 , G01J3/12
Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
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公开(公告)号：US20190285536A1
公开(公告)日：2019-09-19
申请号：US16403642
申请日：2019-05-06
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane
Abstract: A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.
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Patent Agency Ranking
公开(公告)号：US20240278240A1
公开(公告)日：2024-08-22
申请号：US18632183
申请日：2024-04-10
Applicant: Cytek Biosciences, Inc.
Inventor： Glen Krueger , David Vrane , Kuncheng Wang , Qiuta Gu
IPC: B01L3/00 , C12M1/00 , G01N15/01 , G01N15/10 , G01N15/14 , G01N15/1409 , G01N15/1434 , G01N15/149
CPC classification number: B01L3/502761 , B01L3/502715 , C12M47/04 , G01N15/14 , G01N15/1436 , B01L2300/0654 , B01L2300/0663 , B01L2300/0681 , B01L2300/0829 , B01L2300/1805 , B01L2300/1894 , G01N15/01 , G01N2015/1006 , G01N2015/1028 , G01N15/1409 , G01N15/149
Abstract: Methods for a sorting flow cytometer system are disclosed. The method includes forming a stream of a sample biological fluid; charging the stream of the sample biological fluid with a first voltage having a first voltage polarity; breaking a first charged drop off of the stream, wherein the first charged drop is charged with the first voltage; dropping the first charged drop between a first electrostatic charge plate and a second electrostatic charge plate opposite the first electrostatic charge plate, wherein the first electrostatic charge plate and the second electrostatic charge plate are positioned on an angle to form a progressively larger gap between each; deflecting the first charged drop off of a center stream path towards the first electrostatic charge plate to drop along a line with a first angle; and orienting a first pivotal scupper to allow the first charged drop to fall into a first test tube.
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公开(公告)号：US11333597B2
公开(公告)日：2022-05-17
申请号：US15942430
申请日：2018-03-30
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase , Wenbin Jiang
IPC: G01N15/14 , G01J3/02 , G01J3/28 , G01J3/36 , G01N21/64 , G01J3/44 , G01J3/18 , G01J3/14 , G01N21/53 , G01J3/12 , G01N15/00 , G01N15/10
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.
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公开(公告)号：US20210389226A1
公开(公告)日：2021-12-16
申请号：US17412695
申请日：2021-08-26
Applicant: Cytek Biosciences, Inc.
Inventor： Janine Jiang , David Vrane
IPC: G01N15/14
Abstract: In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network. A central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers. Each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure. The monitoring system can detect an advanced failure of components based on the operational parameters being monitored. The monitoring system can also detect an advanced need for repair and maintenance based on the operational parameters being monitored.
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公开(公告)号：US20210096055A1
公开(公告)日：2021-04-01
申请号：US17120120
申请日：2020-12-12
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane
IPC: G01N15/14
Abstract: A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell. A stepper flow control valves are disclosed that apply a physical fluid resistance to a flow of sheath fluid in the flow cytometer. The physical fluid resistance regulates a flow rate of the sheath fluid and thereby regulates a flow rate of sample fluid in the flow cytometer.
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公开(公告)号：US10436697B2
公开(公告)日：2019-10-08
申请号：US15817237
申请日：2017-11-19
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane
Abstract: A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.
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