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公开(公告)号：US20060063168A1
公开(公告)日：2006-03-23
申请号：US11060644
申请日：2005-02-16
申请人： Donna Albertson , Daniel Pinkel , Jane Fridyland , Bing Huey , Antoine Snijders , Joe Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman
发明人： Donna Albertson , Daniel Pinkel , Jane Fridyland , Bing Huey , Antoine Snijders , Joe Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q2600/156 , C12Q2565/601
摘要： The present invention provides a method of detecting nucleotide sequence differences between two nucleic acid samples. The method employs a comparative genomic hybridization (CGH) technique to analyze the sequence differences between the samples. This method permits the identification of small sequence differences (e.g., sequence divergence of 1% or less) in nucleic acid samples of high complexity (e.g., an entire genome).
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公开(公告)号：US20050118634A1
公开(公告)日：2005-06-02
申请号：US11017493
申请日：2004-12-17
CPC分类号： C12Q1/6841 , C12Q1/6809 , C12Q1/6853 , C12Q1/6886 , C12Q2600/158 , C12Q2545/114 , C12Q2545/113 , C12Q2537/143 , C12Q2525/151 , C12Q2563/107
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号：US20060292608A1
公开(公告)日：2006-12-28
申请号：US11431094
申请日：2006-05-08
CPC分类号： C12Q1/6841 , C12Q1/6809 , C12Q1/6853 , C12Q1/6886 , C12Q2600/158 , C12Q2545/114 , C12Q2545/113 , C12Q2537/143 , C12Q2525/151 , C12Q2563/107
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
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公开(公告)号：US20060257895A1
公开(公告)日：2006-11-16
申请号：US11361316
申请日：2006-02-24
申请人： Daniel Pinkel , Joe Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
发明人： Daniel Pinkel , Joe Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/156 , G06F15/025 , Y10S436/813 , C12Q2545/114 , C12Q2537/157
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号：US20070172883A1
公开(公告)日：2007-07-26
申请号：US11727935
申请日：2007-03-29
申请人： Daniel Pinkel , Donna Albertson , Joe Gray
发明人： Daniel Pinkel , Donna Albertson , Joe Gray
CPC分类号： C12Q1/6816 , C12Q1/6837 , G06F19/20 , C12Q2545/101
摘要： The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.
摘要翻译： 本发明提供了确定靶核酸的相对拷贝数和与疾病相关的染色体异常的精确映射的方法。 本发明的方法使用固定在固体表面上的靶核酸，其中包含两组差异标记的核酸的样品与之杂交。 然后使用标准技术检测标记的核酸与固体表面的杂交。
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公开(公告)号：US06417506B1
公开(公告)日：2002-07-09
申请号：US09642243
申请日：2000-08-17
申请人： Daniel Pinkel , Joe Gray , Donna G. Albertson
发明人： Daniel Pinkel , Joe Gray , Donna G. Albertson
IPC分类号： C25D1300
CPC分类号： G01N21/6452 , B01J2219/00513 , B01J2219/00524 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00659 , C12Q1/6825 , G01N21/648 , G01N21/7703 , G01N33/54373 , G01N2201/0833 , G02B6/04
摘要： The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its “sensor end” biological “binding partners” (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers are then discretely addressed to detectors—such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.
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公开(公告)号：US5837196A
公开(公告)日：1998-11-17
申请号：US592779
申请日：1996-01-26
申请人： Daniel Pinkel , Richard L. Segraves , Ye Yz Zhai , Donna G. Albertson , Joe Gray
发明人： Daniel Pinkel , Richard L. Segraves , Ye Yz Zhai , Donna G. Albertson , Joe Gray
IPC分类号： C12N15/09 , C12Q1/68 , G01N21/64 , G01N21/77 , G01N33/53 , G01N33/543 , G01N37/00 , G02B6/04 , G01N21/00 , G01N15/06 , G01N21/29
CPC分类号： G01N21/6452 , C12Q1/6825 , G01N21/7703 , G02B6/04 , B01J2219/00605 , B01J2219/00659 , G01N2201/0833
摘要： The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers are then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.
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公开(公告)号：US20050137389A1
公开(公告)日：2005-06-23
申请号：US10932799
申请日：2004-09-01
申请人： Joe Gray , Daniel Pinkel
发明人： Joe Gray , Daniel Pinkel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6811 , C12Q1/6841 , C12Q1/6853 , C12Q1/6879 , C12Q2600/156 , C12Q2563/131 , C12Q2563/107 , C12Q2525/151
摘要： The invention relates generally to the field of cytogenetics, and more particularly to methods for identifying and classifying chromosomes.
摘要翻译： 本发明一般涉及细胞遗传学领域，更具体地涉及鉴定和分类染色体的方法。
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公开(公告)号：US20050084898A1
公开(公告)日：2005-04-21
申请号：US11002789
申请日：2004-12-03
申请人： Daniel Pinkel , Donna Albertson , Joe Gray
发明人： Daniel Pinkel , Donna Albertson , Joe Gray
IPC分类号： G01N33/53 , C07H19/04 , C07H21/04 , C12N15/09 , C12P19/34 , C12Q1/68 , G01N37/00 , G06F19/20 , G06K9/40 , G06K9/58 , G06K9/60 , G06T1/00 , G06T1/40
CPC分类号： C12Q1/6816 , C12Q1/6837 , G16B25/00 , C12Q2545/101
摘要： The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.
摘要翻译： 本发明提供了确定靶核酸的相对拷贝数和与疾病相关的染色体异常的精确映射的方法。 本发明的方法使用固定在固体表面上的靶核酸，其中包含两组差异标记的核酸的样品与之杂交。 然后使用标准技术检测标记的核酸与固体表面的杂交。
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公开(公告)号：US6146593A
公开(公告)日：2000-11-14
申请号：US899000
申请日：1997-07-24
申请人： Daniel Pinkel , Joe Gray , Donna G. Albertson
发明人： Daniel Pinkel , Joe Gray , Donna G. Albertson
CPC分类号： G01N21/6452 , C12Q1/6825 , G01N21/648 , G01N21/7703 , G01N33/54373 , B01J2219/00513 , B01J2219/00524 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00659 , G01N2201/0833 , G02B6/04
摘要： The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers are then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.
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