公开(公告)号：US20210115379A1

公开(公告)日：2021-04-22

申请号：US17134122

申请日：2020-12-24

Applicant: FUJIFILM CORPORATION

Inventor： Atsushi INADA ,  Hidetoshi TAKAYAMA

IPC: C12M1/26 ,  C12M1/36

Abstract: A cell culture method includes classifying by separating components of a cell suspension containing cell aggregates transferred from a culture vessel according to a size; performing a first collection by collecting cell aggregates having relatively small sizes separated in the classifying, in the culture vessel; dividing cell aggregates having relatively large sizes separated in the classifying; and performing a second collection by collecting the divided cell aggregates into the culture vessel or a collection vessel different from the culture vessel.

公开(公告)号：US20250019419A1

公开(公告)日：2025-01-16

申请号：US18898929

申请日：2024-09-27

Applicant: FUJIFILM Corporation

Inventor： Shinichi NAKAI ,  Atsushi INADA ,  Hiroshi SAKUYAMA ,  Naoto TAKAHASHI

IPC: C07K16/00

Abstract: An object of the present invention is to provide a method in which the amount of mannose 5 in a product can be reduced in the production method for the product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, including culturing cells in a culture container, in which the culturing is a perfusion culture method, Expressions (1) to (5) are satisfied in a production period of a product after reaching a target viable cell density, a variation of VCD during the production period is ±20% or less with respect to an average viable cell density during the production period, and a variation of VCV during the production period is ±20% or less with respect to an average value of the VCV during the production period. 0≤Gln-C≤2.5  Expression (1) 0.5≤Gluc-C≤8.0  Expression (2) 1.0≤NH4-C≤8.0  Expression (3) 50≤VCD≤300  Expression (4) 0.6×105≤VCV≤4.0×105  Expression (5) The meanings of the symbols in the expressions are as defined in the present specification.

公开(公告)号：US20210102158A1

公开(公告)日：2021-04-08

申请号：US17123057

申请日：2020-12-15

Applicant: FUJIFILM CORPORATION

Inventor： Atsushi INADA ,  Nobuhiko KATOU ,  Hidetoshi TAKAYAMA

IPC: C12M1/02 ,  C12M1/04

Abstract: Provided are a cell culture apparatus and a cell culture method which are capable of improving a temperature rising rate of a temperature rising target and enhancing a temperature uniformity of the temperature rising target. The cell culture apparatus includes: a heat transfer medium having a recess part or a plurality of hole parts in a contact surface with a culture vessel for containing a cell suspension; and an air supply port which communicates with the recess part or each of the plurality of hole parts and to which a gas is supplied.

公开(公告)号：US20240002784A1

公开(公告)日：2024-01-04

申请号：US18468223

申请日：2023-09-15

Applicant: FUJIFILM Corporation

Inventor： Atsushi INADA ,  Kosuke TANIGUCHI ,  Naoto TAKAHASHI

IPC: C12N5/00 ,  C12M1/34

CPC classification number: C12N5/0018 ,  C12M41/42 ,  C12N2500/02

Abstract: An object to be achieved by the present invention is to provide a culture method for cells, which makes it possible to suppress oxygen deficiency of cells in a case where the cell is cultured at a high density, and a production method for a useful substance using the culture method for cells. According to the present invention, there is provided a culture method for cells, in which a relationship between a stirring power P/V per unit volume, a dissolved oxygen concentration CL0, and a viable cell density VCD satisfies a condition of Expression 1, in cell culture in which the viable cell density is 60×106 cells/mL or more.


0.0059×exp[0.0243×(P/V)]×CL0/VCD≥1.10×10−17   Expression 1:

In the expression, L0 indicates a dissolved oxygen concentration [mol/mL]; VCD indicates a viable cell density [cells/mL]; and P/V indicates a stirring power [W/m3] per unit volume, which is defined by a predetermined expression.

公开(公告)号：US20180134921A1

公开(公告)日：2018-05-17

申请号：US15869951

申请日：2018-01-12

Applicant: FUJIFILM Corporation

Inventor： Naoki NAKAMURA ,  Takahiro NAKAZAWA ,  Yoshihito HODOSAWA ,  Masanori TAKASE ,  Atsushi INADA ,  Junichi SUGIYAMA

IPC: C09J7/38 ,  B05D1/36 ,  B05D3/04 ,  B05D5/10 ,  C09J183/04

CPC classification number: C09J7/38 ,  B05D1/36 ,  B05D3/0413 ,  B05D5/10 ,  C09J183/04 ,  C09J2201/122 ,  C09J2201/32 ,  C09J2201/36 ,  C09J2201/622 ,  C09J2205/102 ,  C09J2483/00

Abstract: A pressure sensitive adhesive sheet includes a transparent sheet base material, a first pressure sensitive adhesive layer, and a second pressure sensitive adhesive layer, and a pressure sensitive adhesive strength by a pressure of 1 N/cm2 or less is 0 N/25 mm. The first pressure sensitive adhesive layer includes a first silicone-based pressure sensitive adhesive and the second pressure sensitive adhesive layer has a second silicone-based pressure sensitive adhesive and a plurality of protrusions formed of a plurality of fine particles. A first sheet surface is a surface to adhere to an adherend. An average particle diameter of the fine particles is 2 μm or more and 15 μm or less and a coverage ratio of the first sheet surface with the fine particles is 10% or more and 70% or less.

公开(公告)号：US20240240130A1

公开(公告)日：2024-07-18

申请号：US18621692

申请日：2024-03-29

Applicant: FUJIFILM Corporation

Inventor： Atsushi INADA

IPC: C12M1/21 ,  B01D19/04 ,  C12M1/00 ,  C12N5/073

CPC classification number: C12M41/02 ,  B01D19/0409 ,  C12M29/04 ,  C12N5/0604

Abstract: An object of the present invention is to provide a culture method for cells, which makes it possible to suppress clogging of a filtration filter flow channel in a case where cells are cultured at a high density, and a production method for a useful substance using the culture method for cells. According to the present invention, there is provided a culture method for cells, which includes a culture step of culturing a cell suspension having a cell density of 0.7×108 cells/mL or more in a culture tank while introducing a gas into a culture solution and a membrane separation treatment step of passing a cell suspension extracted from the culture tank through a separation membrane to separate the cell suspension into a cell-containing liquid and a permeated liquid, where in the culture method for cells, an anti-foaming component to be added to the culture tank is such that an adding amount thereof per unit culture solution amount and per unit time is 0.70 mg/hour/L or less.

公开(公告)号：US20250019420A1

公开(公告)日：2025-01-16

申请号：US18899965

申请日：2024-09-27

Applicant: FUJIFILM Corporation

Inventor： Takashi KUROSAWA ,  Atsushi INADA ,  Shinichi NAKAI

IPC: C07K16/00

Abstract: An object of the present invention is to provide a method that makes it possible to suppress membrane clogging in a production method for a product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, which includes culturing cells, where the production method for a product is perfusion culture in which a cell density at a time of production of the product is 80×106 cells/mL or more and 300×106 cells/mL or less, where a period of the perfusion culture is 13 days or more and 500 days or less, and in a period of at least one day or more in the period of the perfusion culture, X [mol/L/day], which is an alkali addition rate per day, is controlled in a range of 0≤X

公开(公告)号：US20210071126A1

公开(公告)日：2021-03-11

申请号：US16953340

申请日：2020-11-20

Applicant: FUJIFILM CORPORATION

Inventor： Atsushi INADA ,  Nobuhiko KATOU ,  Hidetoshi TAKAYAMA

IPC: C12M1/00 ,  C12M1/26 ,  C12M3/00 ,  C12M1/34 ,  C12M1/02

Abstract: A cell culture device includes plural three or more of culture vessels, a flow path that connects the plural culture vessels to each other, and a transfer control unit that performs transfer control to transfer a cell suspension housed in any of the plural culture vessels to any other of the plural culture vessels via the flow path.

公开(公告)号：US20200339930A1

公开(公告)日：2020-10-29

申请号：US16928774

申请日：2020-07-14

Applicant: FUJIFILM Corporation

Inventor： Atsushi INADA ,  Hidetoshi TAKAYAMA

IPC: C12M1/06 ,  C12M1/00

Abstract: A cell culture device includes a plurality of culture containers that divide and accommodate a cell suspension; a treatment part that performs a treatment on a certain amount, which corresponds to each of units of treatment, of at least one of a cell suspension accommodated in a culture container, or cells contained in the cell suspension accommodated in the culture container; a mixing part that mixes cell suspensions which are treated in the treatment part and which respectively correspond to each of the plurality of units of treatment; a stirring part that stirs the cell suspension mixed in the mixing part; and a transfer control unit that controls a transfer of the cell suspension to the treatment part, the mixing part, and the stirring part.