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公开(公告)号：US20160237410A1
公开(公告)日：2016-08-18
申请号：US15027169
申请日：2014-10-03
Applicant: GENOMATICA, INC.
Inventor： Stefan Andrae , Michael Patrick Kuchinskas , Jingyi Li , Harish Nagarajan , Priti Pharkya
Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
Abstract translation: 本文描述了与其未修饰的对应物相比，能够分别甲醇或乙醇转化为甲醛或乙醛的至少两倍的非天然NAD +依赖性醇脱氢酶（ADH）。 描述了编码非天然醇脱氢酶的核酸，以及包含核酸的表达构建体和包含核酸或表达构建体的工程化细胞。 还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，用于促进生产的方法 的靶产物或其中间体，包括靶产物或中间体的组合物，以及由目标产物或中间体制成的产品。
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公开(公告)号：US11384340B2
公开(公告)日：2022-07-12
申请号：US16792592
申请日：2020-02-17
Applicant: Genomatica, Inc.
Inventor： Stefan Andrae , Michael Patrick Kuchinskas , Jingyi Li , Harish Nagarajan , Priti Pharkya
Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
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3.发明申请公开(公告)号：US20220177858A1
公开(公告)日：2022-06-09
申请号：US17604722
申请日：2020-04-17
Applicant: Genomatica, Inc.
Inventor： Michael A. Noble , Russell Scott Komor , Jingyi Li
Abstract: Described herein are non-natural olivetol synthase (OLS) variants, nucleic acids, engineered cells, method s for preparing cannabinoids, and compositions thereof. The non-natural olivetol OLS variants form desired cannabinoid precursor and products at increased rates, have higher affinity for pathway substrates, and/or byproducts are formed in lower amounts in their presence, as compared to wild type OLS. The OLS variants can be used to form linear polyketides, and can be expressed in an engineered cell having a pathway to form cannabinoids, which include CBGA, its analogs and derivatives. CBGA can be used for the preparation of cannabigerol (CBG), which can be used in therapeutic compositions.
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公开(公告)号：US20190085303A1
公开(公告)日：2019-03-21
申请号：US15771973
申请日：2016-10-27
Applicant: Genomatica, Inc.
Inventor： Nelson R. Barton , Jingyi Li , Joseph R. Warner , Priti Pharkya
Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
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5.发明申请EUKARYOTIC ORGANISMS AND METHODS FOR INCREASING THE AVAILABILITY OF CYTOSOLIC ACETYL-COA, AND FOR THE PRODUCING 1,3-BUTANEDIOL 审中-公开用于增加CYCOSOLIC ACETYL-COA和生产1,3-丁二醇的可用性的原始有机体和方法公开(公告)号：US20140322779A1
公开(公告)日：2014-10-30
申请号：US14357497
申请日：2012-11-12
Applicant: Genomatica, Inc.
Inventor： Anthony P. Burgard , Mark J. Burk , Robin E. Osterhout , Priti Pharkya , Jingyi Li
IPC: C12P7/18
Abstract: Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway, and methods of using such organisms to produce 1,3-BDO.
Abstract translation: 本文提供的是非天然存在的真核生物，其可被工程化以产生和增加细胞溶质乙酰辅酶A的可用性。 本文还提供具有1,3-丁二醇（1,3-BDO）途径的非天然存在的真核生物，以及使用这些生物体产生1,3-BDO的方法。
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Patent Agency Ranking
公开(公告)号：US11441128B2
公开(公告)日：2022-09-13
申请号：US15771973
申请日：2016-10-27
Applicant: Genomatica, Inc.
Inventor： Nelson R. Barton , Jingyi Li , Joseph R. Warner , Priti Pharkya
Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
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公开(公告)号：US20230265397A1
公开(公告)日：2023-08-24
申请号：US17943482
申请日：2022-09-13
Applicant: Genomatica, Inc.
Inventor： Nelson R. Barton , Jingyi Li , Joseph R. Warner , Priti Pharkya
CPC classification number: C12N9/0006 , C12N9/88 , C12N9/90 , C12Y401/02043 , C12Y503/01027 , C12N15/52 , C12N9/1029 , C12P7/24 , C12Y101/02007 , C12Y203/01085 , C12Y401/02013 , C07K2319/00
Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
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公开(公告)号：US20230037234A1
公开(公告)日：2023-02-02
申请号：US17780421
申请日：2020-11-25
Applicant: Genomatica, Inc.
Inventor： Jingyi Li , Pierre DeWals , Andreas Schirmer , Sankha Ghatak , David Ryan Georgianna
Abstract: The invention relates to engineered microorganisms (e.g., E. coli) and associated improvements for increasing the production cannabinoids (e.g. CBGA) or precursors or derivatives thereof.
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公开(公告)号：US20220127649A1
公开(公告)日：2022-04-28
申请号：US17427019
申请日：2020-01-30
Applicant: Genomatica, Inc.
Inventor： Michael A. Noble , Jonathan Moore , Jingyi Li , Anna Lechner
Abstract: The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing synthesis of olivetolic acid, olivetolic acid derivatives and/or cannabinoids.
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公开(公告)号：US10563180B2
公开(公告)日：2020-02-18
申请号：US15027169
申请日：2014-10-03
Applicant: Genomatica Inc.
Inventor： Stefan Andrae , Michael Patrick Kuchinskas , Jingyi Li , Harish Nagarajan , Priti Pharkya
Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.
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