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    METHANOL DEHYDROGENASE FUSION PROTEINS
    4.
    发明公开
    METHANOL DEHYDROGENASE FUSION PROTEINS 审中-公开

    公开(公告)号：US20230265397A1

    公开(公告)日：2023-08-24

    申请号：US17943482

    申请日：2022-09-13

    申请人： Genomatica, Inc.

    发明人： Nelson R. Barton ,  Jingyi Li ,  Joseph R. Warner ,  Priti Pharkya

    IPC分类号： C12N9/04 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N9/10 ,  C12P7/24

    CPC分类号： C12N9/0006 ,  C12N9/88 ,  C12N9/90 ,  C12Y401/02043 ,  C12Y503/01027 ,  C12N15/52 ,  C12N9/1029 ,  C12P7/24 ,  C12Y101/02007 ,  C12Y203/01085 ,  C12Y401/02013 ,  C07K2319/00

    摘要： Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    RECOMBINANT CELL AND METHOD FOR PRODUCING ISOPRENE
    8.
    发明申请
    RECOMBINANT CELL AND METHOD FOR PRODUCING ISOPRENE 有权
    重组细胞和生产异丙酚的方法

    公开(公告)号：US20150337338A1

    公开(公告)日：2015-11-26

    申请号：US14655934

    申请日：2013-12-26

    申请人： SEKISUI CHEMICAL CO., LTD.

    发明人： Masahiro FURUTANI ,  Akihiro UENISHI ,  Koichiro IWASA

    IPC分类号： C12P5/02 ,  C12N9/90 ,  C12N9/88

    CPC分类号： C12P5/026 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/007 ,  C12Y401/02043 ,  C12Y402/03027 ,  C12Y503/01027 ,  C12Y503/03002 ,  Y02E50/343

    摘要： An object of the present invention is to provide a series of techniques for producing isoprene from methanol or the like. Provided is a recombinant cell prepared by introducing a gene encoding isoprene synthase, into a host cell which is a methylotroph, wherein the gene is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of methane, methanol, methylamine, formic acid, formaldehyde, and formamide. Preferably, it has at least one C1 carbon assimilating pathway selected from the group consisting of a serine pathway, a ribulose monophosphate pathway, and a xylulose monophosphate pathway as a fixing pathway of formaldehyde. Also provided is a method for producing isoprene using the recombinant cell.

    摘要翻译： 本发明的目的是提供一系列用于从甲醇等生产异戊二烯的技术。 提供了通过将编码异戊二烯合酶的基因导入作为甲基营养物的宿主细胞而制备的重组细胞，其中所述基因在宿主细胞中表达，并且重组细胞能够从至少一种选自以下的C1化合物中产生异戊二烯 甲烷，甲醇，甲胺，甲酸，甲醛和甲酰胺组成的组。 优选地，其具有至少一种选自丝氨酸途径，核酮糖一磷酸途径和作为甲醛固定途径的木酮糖单磷酸途径的C1碳同化途径。 还提供了使用重组细胞产生异戊二烯的方法。

    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL
    9.
    发明申请
    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL 审中-公开
    在甲醇存在下增强特定长度脂肪醇的微生物生产方法

    公开(公告)号：US20160376600A1

    公开(公告)日：2016-12-29

    申请号：US15038922

    申请日：2014-11-25

    申请人： GENOMATICA, INC.

    发明人： Robin E. OSTERHOUT ,  Anthony P. BURGARD ,  Priti PHARKYA ,  Stefan ANDRAE

    IPC分类号： C12N15/52 ,  C12N9/04 ,  C12N9/90 ,  C12N9/10 ,  C12P7/04 ,  C12N9/88

    CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12Y101/01244 ,  C12Y202/01003 ,  C12Y401/02043 ,  C12Y503/01027

    摘要： The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

    摘要翻译： 本发明提供具有甲醛固定通道，甲酸同化途径和/或与脂肪醇，脂肪醛，脂肪酸或异丙醇途径组合的甲醇代谢途径的非天然存在的微生物生物，其中所述微生物有选择地产生 脂肪醇，脂肪醛或指定长度的脂肪酸或异丙醇。 提供的微生物有利地增强了用于生产链长特异性脂肪醇，脂肪醛，脂肪酸或异丙醇的底物和/或途径中间体的生产。 在一些方面，本发明的微生物有选择性的基因破坏或酶减弱，其增加脂肪醇，脂肪醛或脂肪酸的产生。 本发明另外提供了使用上述微生物生产脂肪醇，脂肪醛，脂肪酸或异丙醇的方法。