公开(公告)号：US20190010479A1

公开(公告)日：2019-01-10

申请号：US15578488

申请日：2016-05-26

Applicant: GENOMATICA, INC. ,  BRASKEM S.A.

Inventor： Stephanie J. CULLER ,  Robert J. HASELBECK ,  Harish NAGARAJAN ,  Iuri Estrada GOUVEA ,  Daniel Johannes KOCH ,  Mateus Schreiner Garcez LOPES ,  Lucas Pedersen PARIZZI

IPC: C12N9/88 ,  C12P7/16 ,  C07K16/40 ,  C12N15/82 ,  C08F36/06 ,  C12P5/02

Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

公开(公告)号：US20230348865A1

公开(公告)日：2023-11-02

申请号：US17996921

申请日：2021-04-23

Applicant: Genomatica, Inc.

Inventor： Amit Mahendra SHAH ,  Deqiang ZHANG ,  Joseph Roy WARNER ,  Benjamin Matthew GRIFFIN ,  Russell Scott KOMOR ,  Harish NAGARAJAN ,  Sankha GHATAK ,  Toby Howard RICHARDSON

IPC: C12N9/02 ,  C12P13/00 ,  C12P7/04 ,  C12N15/52 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12N9/0008 ,  C12Y102/0103 ,  C12P13/001 ,  C12P7/04 ,  C12N15/52 ,  C12N9/1029 ,  C12Y203/01174 ,  C12Y102/01003 ,  C12N9/88 ,  C12Y402/01017 ,  C12N9/1096

Abstract: The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semi aldehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.

公开(公告)号：US20230159902A1

公开(公告)日：2023-05-25

申请号：US17861036

申请日：2022-07-08

Applicant: Genomatica, Inc.

Inventor： Stefan ANDRAE ,  Michael Patrick KUCHINSKAS ,  Jingyi LI ,  Harish NAGARAJAN ,  Priti PHARKYA

IPC: C12N9/04 ,  C12P7/24 ,  C12P7/18

CPC classification number: C12N9/0006 ,  C12P7/24 ,  C12Y101/01244 ,  C12P7/18 ,  Y02P20/10 ,  C12Y101/01001

Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.