公开(公告)号：US08173070B2

公开(公告)日：2012-05-08

申请号：US10586114

申请日：2005-01-18

申请人： Geoff C. Gerhardt ,  James W. Jorgenson ,  Keith Fadgen

发明人： Geoff C. Gerhardt ,  James W. Jorgenson ,  Keith Fadgen

IPC分类号： G01N30/16

CPC分类号： G01N30/22 ,  G01N30/36 ,  G01N2030/385 ,  Y10T436/2575

摘要： An injection device (10) includes a carrier inlet (40), a sample inlet (46), waste outlet (44) and a chamber outlet (64) attached to separation column (66). Valves (52, 54, 56) are used to control flow such that sample flows into chamber (22) and is carried into the chamber outlet (42).

摘要翻译： 注射装置（10）包括载体入口（40），样品入口（46），废物出口（44）和连接到分离塔（66）的室出口（64）。 阀（52,54,56）用于控制流动，使得样品流入室（22）并被运送到室出口（42）中。

公开(公告)号：US09115815B2

公开(公告)日：2015-08-25

申请号：US13808263

申请日：2011-08-26

申请人： Geoff C. Gerhardt ,  Keith Fadgen

发明人： Geoff C. Gerhardt ,  Keith Fadgen

IPC分类号： F16K11/074 ,  F16K11/00

CPC分类号： F16K11/00 ,  F16K11/0743 ,  Y10T137/0318 ,  Y10T137/86863

摘要： Variable-volume injection valves include a stator and a rotor. The stator has a first port, a second port, and a contact surface with a groove therein. The first port opens into the stator groove. The rotor has a contact surface with a groove therein. The contact surface of the rotor is urged against the contact surface of the stator such that the rotor groove opposes the stator groove with one end of the rotor groove overlapping the stator groove and the opposite end of the rotor groove overlapping the second port of the stator. The overlapping grooves of the rotor and stator provide a fluidic channel between the first and second ports of the stator. The rotor is movable with respect to the stator in order to vary a length of overlap between their overlapping grooves.

摘要翻译： 可变容积喷射阀包括定子和转子。 定子具有第一端口，第二端口和与其中的凹槽的接触表面。 第一端口通向定子槽。 转子具有与其中的凹槽的接触表面。 转子的接触表面被推靠在定子的接触表面上，使得转子槽与定子槽相对，转子槽的一端与定子槽重叠，转子槽的相对端与定子的第二端重叠 。 转子和定子的重叠槽在定子的第一和第二端口之间提供流体通道。 转子相对于定子是可移动的，以便改变其重叠槽之间的重叠长度。

公开(公告)号：US20080121576A1

公开(公告)日：2008-05-29

申请号：US11959602

申请日：2007-12-19

申请人： Geoff C. Gerhardt ,  Keith Fadgen

发明人： Geoff C. Gerhardt ,  Keith Fadgen

IPC分类号： B01D17/12 ,  B01D15/08

CPC分类号： G01F1/363 ,  G01F1/68 ,  G01N30/10 ,  G01N30/30 ,  G01N30/32 ,  G01N2030/324 ,  G01N2030/326

摘要： A method and apparatus for monitoring and controlling the nano-scale flow rate of fluid in the operating flow path of a HPLC system without relying on a nano-scale sensor in the operating flow path. A main flow sensor is disposed in the main flow path between the pump and a flow-divider. A waste flow sensor is disposed in the waste flow path downstream of the splitter. The output signal of the waste flow sensor is subtracted from the output signal of the main flow sensor in a difference circuit. The difference signal is divided by the output signal from the main flow sensor in a divider circuit. The output of the divider circuit represents an empirical split ratio of the flow-divider and is independent of media composition.

摘要翻译： 一种用于在不依赖于操作流程中的纳米级传感器的情况下监视和控制HPLC系统的操作流路中的流体的纳米流量的方法和装置。 主流量传感器设置在泵和分流器之间的主流路中。 废液流量传感器设置在分流器下游的废流路中。 在差分电路中，从主流量传感器的输出信号中减去废液流量传感器的输出信号。 差分信号除以分流器电路中的主流量传感器的输出信号。 分压电路的输出表示分流器的经验分流比，并且与介质组成无关。

公开(公告)号：US20130206240A1

公开(公告)日：2013-08-15

申请号：US13808263

申请日：2011-08-26

申请人： Geoff C. Gerhardt ,  Keith Fadgen

发明人： Geoff C. Gerhardt ,  Keith Fadgen

IPC分类号： F16K11/00

CPC分类号： F16K11/00 ,  F16K11/0743 ,  Y10T137/0318 ,  Y10T137/86863

摘要： Variable-volume injection valves include a stator and a rotor. The stator has a first port, a second port, and a contact surface with a groove therein. The first port opens into the stator groove. The rotor has a contact surface with a groove therein. The contact surface of the rotor is urged against the contact surface of the stator such that the rotor groove opposes the stator groove with one end of the rotor groove overlapping the stator groove and the opposite end of the rotor groove overlapping the second port of the stator. The overlapping grooves of the rotor and stator provide a fluidic channel between the first and second ports of the stator. The rotor is movable with respect to the stator in order to vary a length of overlap between their overlapping grooves.

摘要翻译： 可变容积喷射阀包括定子和转子。 定子具有第一端口，第二端口和与其中的凹槽的接触表面。 第一端口通向定子槽。 转子具有与其中的凹槽的接触表面。 转子的接触表面被推靠在定子的接触表面上，使得转子槽与定子槽相对，转子槽的一端与定子槽重叠，转子槽的相对端与定子的第二端重叠 。 转子和定子的重叠槽在定子的第一和第二端口之间提供流体通道。 转子可相对于定子移动，以便改变其重叠槽之间的重叠长度。

公开(公告)号：US06962077B2

公开(公告)日：2005-11-08

申请号：US10328986

申请日：2002-12-23

申请人： Geoff C. Gerhardt ,  Keith Fadgen

发明人： Geoff C. Gerhardt ,  Keith Fadgen

IPC分类号： G01F1/684 ,  G01N30/32 ,  G01N30/64 ,  G01F1/68

CPC分类号： G01F1/6847 ,  G01N30/64 ,  G01N2030/324

摘要： A method and system for measuring the flow rate of a liquid or gas within a flow channel utilizing a centrally located excitation source and a plurality of sensor means. Said excitation means is comprised of a heating element coupled with an alternating current generator. Of the plurality of sensor means, at least one of said sensors is located in a position upstream of the excitation source location, and additionally a second of said plurality of sensors is located in a position downstream of the excitation source. Instantaneous fluid flow rate is calculated utilizing a high gain differential amplifier electrically coupled to said sensors, wherein the convectively induced inductive gradient of the flowing fluid is compared to the symmetrical zero flow induction gradient. Following such a comparison, a voltage signal proportional to the flow of fluid within the channel is derived.

摘要翻译： 一种用于利用位于中心的激励源和多个传感器装置来测量流动通道内的液体或气体的流量的方法和系统。 所述激励装置包括与交流发电机耦合的加热元件。 在多个传感器装置中，至少一个所述传感器位于激励源位置的上游位置，并且所述多个传感器中的另一个位于激发源的下游位置。 利用电耦合到所述传感器的高增益差分放大器来计算瞬时流体流速，其中流动流体的对流感应感应梯度与对称零流感应梯度进行比较。 在进行这样的比较之后，导出与通道内的流体流量成比例的电压信号。

公开(公告)号：US09396916B2

公开(公告)日：2016-07-19

申请号：US14004236

申请日：2012-03-08

申请人： Geoff C. Gerhardt ,  Keith Fadgen

发明人： Geoff C. Gerhardt ,  Keith Fadgen

IPC分类号： G01D18/00 ,  G12B13/00 ,  H01J49/00 ,  H01J49/04

CPC分类号： H01J49/0009 ,  H01J49/00 ,  H01J49/04

摘要： An electrokinetic pump can be used to deliver calibrant (“lock mass”) ions to a mass spectrometer for calibration of a mass spectrometry system. Electrokinetically controlled calibrant delivery can help to eliminate the need for the more cumbersome mechanisms that are often used for ion delivery. In addition, electrokinetically controlled calibrant delivery can provide for a more user-friendly system in which a calibrant solution can be packaged into a disposable cartridge. Furthermore, when implemented in a microfluidic format, electrokinetically controlled calibrant delivery can be coupled with an electrokinetically controlled separation system, such as capillary electrophoresis (CE), to allow efficient solid-state switching between analytical and calibrant sprays.

摘要翻译： 可以使用电动泵将校准物（“锁定质量”）离子输送到质谱仪用于校准质谱系统。 电动控制的校准物输送可以帮助消除对经常用于离子传递的更繁琐的机制的需要。 此外，电动控制的校准物输送可以提供更加用户友好的系统，其中校准液可以被包装到一次性盒中。 此外，当以微流体形式实施时，电动控制的校准物输送可与电动控制的分离系统（例如毛细管电泳（CE））结合，以允许分析和校准喷雾之间的有效固态切换。

公开(公告)号：US08679332B2

公开(公告)日：2014-03-25

申请号：US11959602

申请日：2007-12-19

申请人： Geoff C. Gerhardt ,  Keith Fadgen

发明人： Geoff C. Gerhardt ,  Keith Fadgen

IPC分类号： B01D15/08

CPC分类号： G01F1/363 ,  G01F1/68 ,  G01N30/10 ,  G01N30/30 ,  G01N30/32 ,  G01N2030/324 ,  G01N2030/326

摘要： A method and apparatus for monitoring and controlling the nano-scale flow rate of fluid in the operating flow path of a HPLC system without relying on a nano-scale sensor in the operating flow path. A main flow sensor is disposed in the main flow path between the pump and a flow-divider. A waste flow sensor is disposed in the waste flow path downstream of the splitter. The output signal of the waste flow sensor is subtracted from the output signal of the main flow sensor in a difference circuit. The difference signal is divided by the output signal from the main flow sensor in a divider circuit. The output of the divider circuit represents an empirical split ratio of the flow-divider and is independent of media composition.

摘要翻译： 一种用于在不依赖于操作流程中的纳米级传感器的情况下监视和控制HPLC系统的操作流路中的流体的纳米流量的方法和装置。 主流量传感器设置在泵和分流器之间的主流路中。 废液流量传感器设置在分流器下游的废流路中。 在差分电路中，从主流量传感器的输出信号中减去废液流量传感器的输出信号。 差分信号除以分流器电路中的主流量传感器的输出信号。 分压电路的输出表示分流器的经验分流比，并且与介质组成无关。

公开(公告)号：US20120171773A1

公开(公告)日：2012-07-05

申请号：US13321666

申请日：2010-05-27

申请人： James P. Murphy ,  Keith Fadgen ,  Geoff C. Gerhardt ,  Angela Doneanu ,  Martha Degen Stapels

发明人： James P. Murphy ,  Keith Fadgen ,  Geoff C. Gerhardt ,  Angela Doneanu ,  Martha Degen Stapels

IPC分类号： G01N30/02

CPC分类号： G01N30/6095 ,  B01D15/22 ,  B01D15/424 ,  B01L3/502715 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2300/0816 ,  B01L2400/0487 ,  G01N30/461 ,  G01N30/606 ,  G01N30/6091 ,  G01N30/7233 ,  G01N30/7266 ,  G01N2030/027 ,  G01N2030/085 ,  G01N2030/8881 ,  Y10T29/49826

摘要： A method of analyzing samples includes loading a sufficient quantity of the sample onto a trap column to overload the trap column; heating an analytical column and the trap column to a greater temperature than the analytical column; and pumping a solvent, to the trap column, having a solvent composition profile that, in cooperation with a temperature differential, causes at least some of the components to elute sequentially from the trap column to the analytical column and focus on the analytical column prior to eluting from the analytical column; or optionally: loading a small-molecule sample onto a cooled portion of an analytical column; heating the analytical column; and pumping a solvent, to the heated analytical column, to elute the components from the analytical column. Chromatographic separation includes: a trap column; a separation column; a trap-column heater; a separation-column heater; a solvent pump unit; and a control unit can be used.

摘要翻译： 分析样品的方法包括将足够量的样品加载到陷阱柱上以使捕获柱过载; 将分析柱和捕集柱加热至比分析柱更大的温度; 并将溶剂泵送到捕集塔，其具有溶剂组成分布，其与温差相配合使至少一些组分从捕集塔顺序洗脱至分析柱，并在分离柱之前聚焦在分析柱上 从分析柱洗脱; 或任选地：将小分子样品加载到分析柱的冷却部分上; 加热分析柱; 并将溶剂泵入加热的分析柱，以从分析柱中洗脱组分。 色谱分离包括：陷阱柱; 分离柱; 一个陷阱加热器; 分离柱加热器; 溶剂泵单元; 并且可以使用控制单元。

公开(公告)号：US20110272855A1

公开(公告)日：2011-11-10

申请号：US13125182

申请日：2009-10-28

申请人： Joseph A. Luongo ,  Michael Budnick ,  Mark W. Moeller ,  James P. Murphy ,  Geoff C. Gerhardt ,  Keith Fadgen ,  Joseph D. Michienzi

发明人： Joseph A. Luongo ,  Michael Budnick ,  Mark W. Moeller ,  James P. Murphy ,  Geoff C. Gerhardt ,  Keith Fadgen ,  Joseph D. Michienzi

IPC分类号： B29C59/02

CPC分类号： F16K11/074 ,  F16K25/005 ,  G01N2030/202

摘要： Described are techniques for fabricating one or more parts of a valve used in a liquid chromatography system. At least one of a rotor and a stator are provided. The rotor is included in the valve and has a first surface facing a stator. The stator is included in the valve and has a second surface facing the rotor. A pattern is formed in at least one of the first surface and the second surface. Forming the pattern includes compressing the at least one surface by applying pressure thereto causing displacement of material to form at least one groove.

摘要翻译： 描述了用于制造用于液相色谱系统的阀的一个或多个部分的技术。 提供转子和定子中的至少一个。 转子包括在阀中并且具有面向定子的第一表面。 定子包括在阀中并且具有面向转子的第二表面。 在第一表面和第二表面中的至少一个中形成图案。 形成图案包括通过施加压力来压缩至少一个表面，引起材料的移位以形成至少一个凹槽。

公开(公告)号：US07332087B2

公开(公告)日：2008-02-19

申请号：US10723973

申请日：2003-11-26

申请人： Geoff C. Gerhardt ,  Keith Fadgen

发明人： Geoff C. Gerhardt ,  Keith Fadgen

IPC分类号： B01D15/08

CPC分类号： G01F1/363 ,  G01F1/68 ,  G01N30/10 ,  G01N30/30 ,  G01N30/32 ,  G01N2030/324 ,  G01N2030/326

摘要： A method and apparatus for monitoring and controlling the nano-scale flow rate of fluid in the operating flow path of a HPLC system without relying on a nano-scale sensor in the operating flow path. A main flow sensor is disposed in the main flow path between the pump and a flow-divider. A waste flow sensor is disposed in the waste flow path downstream of the splitter. The output signal of the waste flow sensor is subtracted from the output signal of the main flow sensor in a difference circuit. The difference signal is divided by the output signal from the main flow sensor in a divider circuit. The output of the divider circuit represents an empirical split ratio of the flow-divider and is independent of media composition.

摘要翻译： 一种用于监测和控制HPLC系统的操作流路中的流体的纳米级流速的方法和装置，而不依赖于操作流路中的纳米级传感器。 主流量传感器设置在泵和分流器之间的主流路中。 废液流量传感器设置在分流器下游的废流路中。 在差分电路中，从主流量传感器的输出信号中减去废液流量传感器的输出信号。 差分信号除以分流器电路中的主流量传感器的输出信号。 分压电路的输出表示分流器的经验分流比，并且与介质组成无关。