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公开(公告)号：US08173070B2
公开(公告)日：2012-05-08
申请号：US10586114
申请日：2005-01-18
IPC分类号： G01N30/16
CPC分类号： G01N30/22 , G01N30/36 , G01N2030/385 , Y10T436/2575
摘要： An injection device (10) includes a carrier inlet (40), a sample inlet (46), waste outlet (44) and a chamber outlet (64) attached to separation column (66). Valves (52, 54, 56) are used to control flow such that sample flows into chamber (22) and is carried into the chamber outlet (42).
摘要翻译： 注射装置（10）包括载体入口（40），样品入口（46），废物出口（44）和连接到分离塔（66）的室出口（64）。 阀（52,54,56）用于控制流动，使得样品流入室（22）并被运送到室出口（42）中。
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2.发明授权
公开(公告)号：US5131998A
公开(公告)日：1992-07-21
申请号：US612662
申请日：1990-11-13
CPC分类号： B01D15/08 , G01N27/44743 , G01N27/44773 , G01N30/62 , G01N30/02 , G01N30/10 , G01N30/20 , G01N30/34
摘要： Disclosed is a two-dimensional separation system, comprising a liquid chromatography column having an outlet port; a capillary electrophoresis capillary having an inlet end and an outlet end; buffer supply means for supplying buffer to the capillary; and valve means connecting the capillary inlet end to the chromatography column outlet port and to the buffer supply means, the valve means switchable between a first configuration providing fluid to the capillary inlet end from the buffer supply means and a second configuration providing fluid to the capillary inlet end from the chromatography column. Methods of carrying out two-dimensional separations employing the system are also disclosed.
摘要翻译： 公开了一种二维分离系统，其包括具有出口的液相色谱柱; 具有入口端和出口端的毛细管电泳毛细管; 用于向毛细管供应缓冲液的缓冲器供应装置; 以及阀装置，其将毛细管入口端连接到色谱柱出口和缓冲器供应装置，所述阀装置可在从缓冲器供应装置向毛细管入口端提供流体的第一构造和向毛细管提供流体的第二构造之间切换 入口端从色谱柱。 还公开了使用该系统进行二维分离的方法。
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公开(公告)号：US5269900A
公开(公告)日：1993-12-14
申请号：US581683
申请日：1990-09-13
IPC分类号： G01N21/64 , G01N21/78 , G01N27/447 , G01N27/26
CPC分类号： G01N27/44704 , G01N27/44721
摘要： A device and method useful for high-speed electrophoresis is disclosed. The device includes an on-column optical gating apparatus for high-frequency modulation of sample introduction, a variable resistance capillary, and a high voltage power supply to induce differential migration. The device separates charged molecules, including complex organic compounds, quickly and efficiently. The device can be used in multiplex chromatographic and electrophoretic separations.
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4.发明授权
公开(公告)号：US5240577A
公开(公告)日：1993-08-31
申请号：US891860
申请日：1992-06-01
CPC分类号： G01N30/463 , B01D15/08 , G01N27/44743 , G01N27/44773 , G01N30/62 , G01N30/02 , G01N30/10 , G01N30/20 , G01N30/34
摘要： Disclosed is a two-dimensional separation system, comprising a liquid chromatography column having an outlet port; a capillary electrophoresis capillary having an inlet end and an outlet end; buffer supply means for supplying buffer to the capillary; and valve means connecting the capillary inlet end to the chromatography column outlet port and to the buffer supply means, the valve means switchable between a first configuration providing fluid to the capillary inlet end from the buffer supply means and a second configuration providing fluid to the capillary inlet end from the chromatography column. Methods of carrying out two-dimensional separations employing the system are also disclosed.
摘要翻译： 公开了一种二维分离系统，其包括具有出口的液相色谱柱; 具有入口端和出口端的毛细管电泳毛细管; 用于向毛细管供应缓冲液的缓冲器供应装置; 以及阀装置，其将毛细管入口端连接到色谱柱出口和缓冲器供应装置，所述阀装置可在从缓冲器供应装置向毛细管入口端提供流体的第一构造和向毛细管提供流体的第二构造之间切换 入口端从色谱柱。 还公开了使用该系统进行二维分离的方法。
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公开(公告)号：US5389221A
公开(公告)日：1995-02-14
申请号：US28678
申请日：1993-03-09
IPC分类号： G01N27/447 , G01N30/02 , G01N30/38 , G01N30/46 , G01N27/26
CPC分类号： G01N30/38 , G01N27/44743 , G01N27/44773 , G01N2030/027 , G01N2030/383 , G01N30/02 , G01N30/461 , G01N30/463
摘要： A combination liquid chromatography and capillary electrophoresis separation system is disclosed. The system comprises a flow gating interface having an effluent channel and a gating channel formed therein. The gating channel transversely intersects the effluent channel at an intersection portion so that the channels are in fluid communication with one another. The intersection portion divides the effluent channel into an upstream portion and a downstream portion, and divides the gating channel into an upstream portion and a downstream portion. A liquid chromatography column is connected to the effluent channel upstream portion and an electrophoresis capillary is connected to the effluent channel downstream portion. A flush solution inlet line is connected to said gating channel upstream portion, and a flush solution outlet line is connected to the gating channel downstream portion. A valve regulates the flow of flush solution from the flush solution inlet line to the gating channel upstream portion. The intersection portion is configured so that the rate of flow of effluent from the effluent channel upstream portion to the effluent channel downstream portion decreases as the rate of flow of flush solution in the gating channel increases.
摘要翻译： 公开了液相色谱和毛细管电泳分离系统的组合。 该系统包括具有流出通道和形成在其中的门控通道的流动门控接口。 门控通道在交叉部分处与流出通道横向相交，使得通道彼此流体连通。 交叉部分将排放通道分成上游部分和下游部分，并将浇口通道分成上游部分和下游部分。 液相色谱柱连接到流出通道上游部分，电泳毛细管连接到流出物通道下游部分。 冲洗溶液入口管线连接到所述浇口通道上游部分，并且冲洗溶液出口管线连接到浇口通道下游部分。 调节冲洗溶液从冲洗溶液入口管线到浇口通道上游部分的流动。 交叉部分被配置成使得流出物从流出物通道上游部分到流出物通道下游部分的流动速率随着浇口通道中冲洗溶液流速的增加而减小。
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6.发明授权Capillary separation system with electric field assisted post separation mixing 失效毛细管分离系统，电场辅助后分离混合
公开(公告)号：US4936974A
公开(公告)日：1990-06-26
申请号：US266547
申请日：1988-11-03
申请人： Donald J. Rose , James W. Jorgenson
发明人： Donald J. Rose , James W. Jorgenson
IPC分类号： G01N27/447
CPC分类号： G01N27/44721
摘要： A capillary zone electrophoresis (CZE) system provides for rapid, non-turbulent post-separation diffusional mixing of sample effluent with a fluorogenic-labelling reagent permitting sensitive detection of well-defined sample component zones. A separation capillary extends into a mixing capillary so as to define an annular gap therebetween. The effluent of the separation capillary is mixed with the labelling reagent, which is introduced through the annular gap. A power supply and opposing electrodes establish an electric field which induces electro-osmotic flow of the sample and charge-related differential electrophoretic migration to define component zones. The electric field also causes the separation capillary effluent to diverge as it issues into the mixing capillary so as to facilitate diffusional mixing with the reagent fluid flow without causing significant turbulence. Thus, fluorescence labelling is effective with minimum zone broadening. This system combines the high resolving power of CZE separation with the sensitivity of labelled fluorescence detection to attain an improved system for analyzing biological samples.
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公开(公告)号：US5496460A
公开(公告)日：1996-03-05
申请号：US359614
申请日：1994-12-20
IPC分类号： G01N27/447 , G01N30/02 , G01N30/38 , G01N30/46 , G01N27/26
CPC分类号： G01N30/38 , G01N27/44743 , G01N27/44773 , G01N2030/027 , G01N2030/383 , G01N30/02 , G01N30/461 , G01N30/463
摘要： A combination liquid chromatography and capillary electrophoresis separation system is disclosed. The system comprises a flow gating interface having an effluent channel and a gating channel formed therein. The gating channel transversely intersects the effluent channel at an intersection portion so that the channels are in fluid communication with one another. The intersection portion divides the effluent channel into an upstream portion and a downstream portion, and divides the gating channel into an upstream portion and a downstream portion. A liquid chromatography column is connected to the effluent channel upstream portion and an electrophoresis capillary is connected to the effluent channel downstream portion. A flush solution inlet line is connected to said gating channel upstream portion, and a flush solution outlet line is connected to the gating channel downstream portion. A valve regulates the flow of flush solution from the flush solution inlet line to the gating channel upstream portion. The intersection portion is configured so that the rate of flow of effluent from the effluent channel upstream portion to the effluent channel downstream portion decreases as the rate of flow of flush solution in the gating channel increases.
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公开(公告)号：US5115131A
公开(公告)日：1992-05-19
申请号：US700572
申请日：1991-05-15
IPC分类号： G01N27/62 , G01N27/447 , G01N30/72 , H01J49/10 , H01J49/16
CPC分类号： H01J49/165 , G01N30/72
摘要： A method of generating an electrospray from a solution is disclosed which is useful for electrospraying at low flow rates, for electrospraying directly into a vacuum, or both. The method comprises the steps of causing the solution to flow through a capillary tube to an outlet opening formed in the terminal portion thereof. The capillary tube terminal portion has an inner diameter of not more than 50 micrometers and an outer diameter of not more than 150 micrometers. An electrical potential difference is provided between the capillary tube terminal portion and a conductor spaced from said terminal portion, with the potential sufficient to cause the solution to electrospray from the capillary outlet.The method is particularly useful for detecting analytes in the solution being electrosprayed by mass spectral analysis.
摘要翻译： 公开了一种从溶液中产生电喷雾的方法，其可用于以低流速进行电喷雾，用于直接电喷雾至真空中或两者。 该方法包括使溶液流过毛细管至其末端部分形成的出口的步骤。 毛细管端子部的内径不大于50微米，外径不大于150微米。 在毛细管端子部分和与所述端子部分间隔开的导体之间提供电势差，具有足以使溶液从毛细管出口电喷雾的电位。 该方法对于通过质谱分析电喷雾的溶液中的分析物特别有用。
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