公开(公告)号：US20080004355A1

公开(公告)日：2008-01-03

申请号：US11793959

申请日：2005-12-27

申请人： Naoya Wada ,  Takashi Okamoto ,  Keiji Tanigaki ,  Hirofumi Doi ,  Kensaku Imai

发明人： Naoya Wada ,  Takashi Okamoto ,  Keiji Tanigaki ,  Hirofumi Doi ,  Kensaku Imai

IPC分类号： A61K35/00 ,  A61P35/00 ,  C12N15/63 ,  C12N5/00 ,  C12N9/10 ,  C12Q1/34 ,  C12Q1/48 ,  C12Q1/68

CPC分类号： C12Y207/07049 ,  A61K45/06 ,  C12N9/1241 ,  G01N33/574 ,  G01N2333/9125 ,  G01N2333/916 ,  G01N2500/02

摘要： A method is described for enhancing degradation of telomerase. This method includes inhibiting the binding of TERT (telomerase catalytic subunit) to USP21, the binding of NEDD8-conjugated TERT to USP21, or the NEDD8 deconjugation by USP21 from NEDD8-conjugated TERT. A method of inhibiting telomerase activity is also described. The method includes utilizing the method of enhancing the degradation. A method of identifying a compound that inhibits the binding or the NEDD8 deconjugation is further described. An agent for inhibiting telomerase activity is described. An agent for preventing and/or treating diseases attributable to the enhanced telomerase activity is described and includes an inhibitory agent. Also, a method of preventing and/or treating diseases is described and includes using the inhibition method or the inhibitory agent. Further, a reagent kit is described.

摘要翻译： 描述了一种增强端粒酶降解的方法。 该方法包括抑制TERT（端粒酶催化亚单位）与USP21的结合，NEDD8缀合的TERT与USP21的结合，或者通过NEDD8缀合的TERT的USP21的NEDD8解缀。 还描述了抑制端粒酶活性的方法。 该方法包括利用增强降解的方法。 进一步描述鉴定抑制结合或NEDD8解缀合的化合物的方法。 描述了抑制端粒酶活性的药剂。 描述了用于预防和/或治疗归因于增强的端粒酶活性的疾病的药剂，并且包括抑制剂。 此外，描述了预防和/或治疗疾病的方法，并且包括使用抑制方法或抑制剂。 此外，描述了试剂盒。

公开(公告)号：US20060276387A1

公开(公告)日：2006-12-07

申请号：US10544061

申请日：2004-11-26

申请人： Hirofumi Doi ,  Kensaku Imai ,  Naoya Wada

发明人： Hirofumi Doi ,  Kensaku Imai ,  Naoya Wada

IPC分类号： A61K38/22 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C07K14/62 ,  G01N33/74 ,  G01N2500/02

摘要： A method for promoting insulin gene transcription, which comprises the step of inhibiting binding of IPF1 and any one of proteins selected from the following group: (i) HNF3G, (ii) PHF1, and (iii) DLX4; and a method for screening a substance that promotes insulin gene transcription, which comprises the step of bringing a test substance into contact with IPF1 and/or any one of proteins selected from the following group under a condition that allows the binding of IPF1 and said protein and then determining whether or not the test substance inhibits the binding of IPF1 and said protein by detecting presence or absence, or change of a signal and/or a marker generated by the binding of IPF1 and said protein in a system in which the signal and/or the marker can be detected: (i) HNF3G, (ii) PHF1, and (iii) DLX4.

摘要翻译： 一种促进胰岛素基因转录的方法，其包括抑制IPF1与选自以下组中的任何一种蛋白质结合的步骤：（i）HNF3G，（ii）PHF1和（iii）DLX4; 以及用于筛选促进胰岛素基因转录的物质的方法，其包括在允许IPF1和所述蛋白质结合的条件下使测试物质与IPF1和/或选自以下组的任何一种蛋白质接触的步骤 然后通过检测存在或不存在检测物质来抑制IPF1和所述蛋白质的结合，或者通过IPF1和所述蛋白质的结合产生的信号和/或标记的变化来抑制IPF1和所述蛋白质的结合，其中信号和 /或可以检测标记：（i）HNF3G，（ii）PHF1和（iii）DLX4。

公开(公告)号：US20070105796A1

公开(公告)日：2007-05-10

申请号：US10563074

申请日：2005-04-28

申请人： Naoya Wada ,  Takashi Okamoto ,  Keiji Tanigaki ,  Hirofumi Doi ,  Yasuhiro Kikuchi ,  Kensaku Imai

发明人： Naoya Wada ,  Takashi Okamoto ,  Keiji Tanigaki ,  Hirofumi Doi ,  Yasuhiro Kikuchi ,  Kensaku Imai

IPC分类号： A61K48/00

CPC分类号： C12N9/1205

摘要： The present invention provides a method for inhibiting the activation of telomerase and an agent for inhibiting the activation of telomerase, a method for inhibiting telomerase activity and an agent for inhibiting telomerase activity, a method for preventing and/or a method for treating a cancer disease, an agent for preventing and/or an agent for treating a cancer disease, all of which comprises inhibiting the binding of MAPKAPK3 to TERT that is a catalytic subunit of telomerase or inhibiting the phosphorylation of TERT by active MAPKAPK3; a method of identifying a compound that inhibits the binding of MAPKAPK3 to TERT or a compound that inhibits the phosphorylation of TERT by active MAPKAPK3; and a reagent kit.

摘要翻译： 本发明提供抑制端粒酶活化的方法和抑制端粒酶活化的试剂，抑制端粒酶活性的方法和抑制端粒酶活性的方法，预防和/或治疗癌症的方法 ，用于预防和/或治疗癌症疾病的药物，所有这些都包括抑制MAPKAPK3与作为端粒酶催化亚基的TERT的结合或抑制活性MAPKAPK3的TERT磷酸化; 鉴定抑制MAPKAPK3与TERT结合的化合物或抑制活性MAPKAPK3磷酸化TERT的化合物的方法; 和试剂盒。

公开(公告)号：US20050164198A1

公开(公告)日：2005-07-28

申请号：US10513928

申请日：2003-05-12

申请人： Kensaku Imai ,  Hisayuki Horai ,  Hirofumi Doi

发明人： Kensaku Imai ,  Hisayuki Horai ,  Hirofumi Doi

IPC分类号： C12M1/00 ,  C12Q1/68 ,  G06F17/30 ,  G06F19/22 ,  H04R23/00 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50

CPC分类号： H04R23/008 ,  G16B30/00

摘要： All DNA's of a mutant of a sequencing target organic species (e.g., a resistant bacterium, a tumor cell, or a virus) are extracted, and shotgun libraries are generated for the DNA's. DNA fragments are sequentially fetched from these libraries, and each fragment is subjected to sequencing using an automated DNA sequencer. Fragment sequence information such as base sequences of the respective DNA fragments of the mutant output from the sequencer is stored in a fragment sequence database.

摘要翻译： 提取测序目标有机物种（例如抗性细菌，肿瘤细胞或病毒）的突变体的所有DNA，并为DNA产生猎枪文库。 从这些文库顺序取出DNA片段，并使用自动化DNA测序仪对每个片段进行测序。 片段序列信息，例如从定序器输出的突变体的各个DNA片段的碱基序列存储在片段序列数据库中。

公开(公告)号：US20050130224A1

公开(公告)日：2005-06-16

申请号：US10516133

申请日：2003-06-02

申请人： Seiji Saito ,  Kazuki Ono ,  Mitsuhito Wada ,  Kensaku Imai ,  Shinya Hosogi ,  Takashi Shimada

发明人： Seiji Saito ,  Kazuki Ono ,  Mitsuhito Wada ,  Kensaku Imai ,  Shinya Hosogi ,  Takashi Shimada

IPC分类号： C07K1/00 ,  G01N33/53 ,  G06F17/30 ,  G06F19/16 ,  G06F19/18 ,  G06F19/22 ,  G06G7/48 ,  G06G7/58

CPC分类号： G16B20/00 ,  G16B15/00 ,  G16B30/00

摘要： Objective sequence data (10) which is primary sequence information on an objective protein is entered in an interaction site predicting device by the user. A secondary structure prediction simulation is executed on the objective sequence data (10) entered for secondary structure prediction programs (20a to 20d) that predict a secondary structure of a protein from primary sequence information of the protein. Results of secondary structure prediction (30a to 30d) from the respective secondary structure prediction programs (20a to 20d) are compared (60). Based on the comparison result, frustration of a local portion in the primary sequence information of the objective protein is calculated (70). An interaction site of the objective protein is predicted from the calculated frustration of the local portion (80).

摘要翻译： 作为目标蛋白质的主序列信息的目标序列数据（10）由用户输入到交互站点预测装置。 对从二级结构预测程序（20a到20d）输入的目标序列数据（10）执行二级结构预测模拟，其从蛋白质的一级序列信息预测蛋白质的二级结构。 比较来自各个二级结构预测程序（20a至20d）​​的二级结构预测（30a至30d）的结果（60）。 基于比较结果，计算目标蛋白的一级序列信息中局部部分的挫败（70）。 目标蛋白质的相互作用位点是从计算出的局部部分的挫折中预测的（80）。

公开(公告)号：US06708119B2

公开(公告)日：2004-03-16

申请号：US09785269

申请日：2001-02-20

申请人： Kensaku Imai ,  Masato Kitajima

发明人： Kensaku Imai ,  Masato Kitajima

IPC分类号： G06F1900

CPC分类号： C12N15/10

摘要： An automatic DNA fragment removing method aims at removing a vector unit base sequence, that is, a base sequence in a portion of a vector, with precision from the DNA integrated into the vector and processed in a cloning process, so as to obtain a target DNA fragment in an exact structure. Depending on the vector and the restriction enzyme used for cleaving the vector and generating an object DNA fragment, a retrieval key is generated to retrieve a vector unit from the DNA base sequence obtained as the cloning process. Using the generated retrieval key, the junction between the DNA fragment and the vector unit is specified, and the specified junction and the base sequence outside the junction are automatically removed as the vector unit.

摘要翻译： 自动DNA片段去除方法的目的是从载体整合到载体中的DNA的一部分中去除载体单元碱基序列，即载体的一部分的碱基序列，并在克隆过程中进行处理，以获得目标 DNA片段的确切结构。 根据用于切割载体的载体和限制性内切酶和产生对象DNA片段，产生检索关键码，以从作为克隆过程获得的DNA碱基序列中检索载体单元。 使用生成的检索键，指定DNA片段和向量单位之间的连接点，并且将结点外的指定连接点和基本序列作为向量单元自动去除。

公开(公告)号：US5891632A

公开(公告)日：1999-04-06

申请号：US685959

申请日：1996-07-22

申请人： Kensaku Imai

发明人： Kensaku Imai

IPC分类号： C12N15/09 ,  C12Q1/68 ,  G06F17/30 ,  G06F19/00 ,  G06F19/22 ,  G06Q50/00 ,  G06Q90/00

CPC分类号： C12Q1/6869

摘要： In a DNA base sequencing, a consensus sequence is obtained by linking a plurality of DNA fragments obtained by a DNA sequencer, and a base sequence to be edited is determined in the consensus sequence. Then, a trace corresponding to the determined base sequence to be edited is identified among traces obtained by the DNA sequencer. The identified trace is displayed in correspondence with the base sequence to be edited. At this time, the base sequence to be edited is displayed so that an interval between bases becomes even.

摘要翻译： 在DNA碱基测序中，通过连接由DNA测序仪获得的多个DNA片段获得共有序列，并且在共有序列中确定待编辑的碱基序列。 然后，在由DNA测序仪获得的踪迹中识别与待编辑的确定的碱基序列相对应的踪迹。 所识别的跟踪将与要编辑的基本序列相对应地显示。 此时，显示要编辑的基本序列，使得基底之间的间隔变得均匀。