公开(公告)号：US20050164198A1

公开(公告)日：2005-07-28

申请号：US10513928

申请日：2003-05-12

申请人： Kensaku Imai ,  Hisayuki Horai ,  Hirofumi Doi

发明人： Kensaku Imai ,  Hisayuki Horai ,  Hirofumi Doi

IPC分类号： C12M1/00 ,  C12Q1/68 ,  G06F17/30 ,  G06F19/22 ,  H04R23/00 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50

CPC分类号： H04R23/008 ,  G16B30/00

摘要： All DNA's of a mutant of a sequencing target organic species (e.g., a resistant bacterium, a tumor cell, or a virus) are extracted, and shotgun libraries are generated for the DNA's. DNA fragments are sequentially fetched from these libraries, and each fragment is subjected to sequencing using an automated DNA sequencer. Fragment sequence information such as base sequences of the respective DNA fragments of the mutant output from the sequencer is stored in a fragment sequence database.

摘要翻译： 提取测序目标有机物种（例如抗性细菌，肿瘤细胞或病毒）的突变体的所有DNA，并为DNA产生猎枪文库。 从这些文库顺序取出DNA片段，并使用自动化DNA测序仪对每个片段进行测序。 片段序列信息，例如从定序器输出的突变体的各个DNA片段的碱基序列存储在片段序列数据库中。

公开(公告)号：US20050154690A1

公开(公告)日：2005-07-14

申请号：US10503387

申请日：2003-02-04

申请人： Kiyoshi Nitta ,  Hirofumi Doi ,  Yasuhiro Kikuchi ,  Hisayuki Horai

发明人： Kiyoshi Nitta ,  Hirofumi Doi ,  Yasuhiro Kikuchi ,  Hisayuki Horai

IPC分类号： G06F17/22 ,  G06F17/30 ,  G06F17/24 ,  G06F17/21 ,  G06F15/00 ,  G06F17/00 ,  G06N5/02 ,  G06F17/27

CPC分类号： G06F17/2217 ,  G06F16/30

摘要： In the present invention, a textual document is syntactically analyzed and knowledge is constructed from a single word or plural words. The knowledge is then marked, from the broken down knowledge (represented by the underscores in FIG. 1) or from a part-of-speech, as a related object (node) or a relation (edge) (represented by ‘n’ or ‘e’ shown in FIG. 1). In other words, in the present invention a textual document is treated as knowledge constructed from a single word or plural words. The knowledge extracted from the textual document is structured to form a knowledge structure (such as a graph structure constituted from nodes and edges). At least one link can be established between each of the knowledge structure elements and a semantically closest concept entry in a hierarchical concept dictionary.

摘要翻译： 在本发明中，文本文档被语法分析，并从单个单词或多个单词构成知识。 然后将知识从分解的知识（由图1中的下划线表示）或从词性部分标记为相关对象（节点）或关系（边缘）（由“n”或 'e'）。 换句话说，在本发明中，文本文档被视为从单个单词或多个单词构成的知识。 从文本文档中提取的知识被构造成形成知识结构（例如由节点和边缘构成的图形结构）。 可以在每个知识结构元素和分层概念词典中的语义上最接近的概念条目之间建立至少一个链接。

公开(公告)号：US20050146951A1

公开(公告)日：2005-07-07

申请号：US10499911

申请日：2003-01-06

申请人： Hisayuki Horai ,  Kiyoshi Nitta ,  Hirofumi Doi

发明人： Hisayuki Horai ,  Kiyoshi Nitta ,  Hirofumi Doi

IPC分类号： G06F17/30 ,  G06F19/24 ,  G06F19/28 ,  G11C7/00

CPC分类号： G16B50/00

摘要： A user designates two targets a relation of which the user is to search as a search start target and a search end target, respectively. The user also designates various search conditions. A binary relation database that stores binary relational information extracted, in advance, from various types of biomolecular databases (e.g., databases that store sequence information on DNAs and the like, structural information on proteins and the like, etc), compound databases (e.g., databases that store characteristics of organic compounds and the like), and literature databases (e.g., databases that store technical literatures, papers, and the like) is accessed, and knowledge between the search start target and the search end target is automatically searched.

摘要翻译： 用户分别将用户要搜索的关系的两个目标指定为搜索开始目标和搜索结束目标。 用户还指定各种搜索条件。 预先从各种类型的生物分子数据库中提取二进制关系信息的二元关系数据库（例如，存储关于DNA等的序列信息的数据库，关于蛋白质等的结构信息等），化合物数据库（例如， 存储有机化合物的特征的数据库等），以及文献数据库（例如存储技术文献，论文等的数据库），自动搜索搜索开始对象与搜索结束目标之间的知识。

公开(公告)号：US20080004355A1

公开(公告)日：2008-01-03

申请号：US11793959

申请日：2005-12-27

申请人： Naoya Wada ,  Takashi Okamoto ,  Keiji Tanigaki ,  Hirofumi Doi ,  Kensaku Imai

发明人： Naoya Wada ,  Takashi Okamoto ,  Keiji Tanigaki ,  Hirofumi Doi ,  Kensaku Imai

IPC分类号： A61K35/00 ,  A61P35/00 ,  C12N15/63 ,  C12N5/00 ,  C12N9/10 ,  C12Q1/34 ,  C12Q1/48 ,  C12Q1/68

CPC分类号： C12Y207/07049 ,  A61K45/06 ,  C12N9/1241 ,  G01N33/574 ,  G01N2333/9125 ,  G01N2333/916 ,  G01N2500/02

摘要： A method is described for enhancing degradation of telomerase. This method includes inhibiting the binding of TERT (telomerase catalytic subunit) to USP21, the binding of NEDD8-conjugated TERT to USP21, or the NEDD8 deconjugation by USP21 from NEDD8-conjugated TERT. A method of inhibiting telomerase activity is also described. The method includes utilizing the method of enhancing the degradation. A method of identifying a compound that inhibits the binding or the NEDD8 deconjugation is further described. An agent for inhibiting telomerase activity is described. An agent for preventing and/or treating diseases attributable to the enhanced telomerase activity is described and includes an inhibitory agent. Also, a method of preventing and/or treating diseases is described and includes using the inhibition method or the inhibitory agent. Further, a reagent kit is described.

摘要翻译： 描述了一种增强端粒酶降解的方法。 该方法包括抑制TERT（端粒酶催化亚单位）与USP21的结合，NEDD8缀合的TERT与USP21的结合，或者通过NEDD8缀合的TERT的USP21的NEDD8解缀。 还描述了抑制端粒酶活性的方法。 该方法包括利用增强降解的方法。 进一步描述鉴定抑制结合或NEDD8解缀合的化合物的方法。 描述了抑制端粒酶活性的药剂。 描述了用于预防和/或治疗归因于增强的端粒酶活性的疾病的药剂，并且包括抑制剂。 此外，描述了预防和/或治疗疾病的方法，并且包括使用抑制方法或抑制剂。 此外，描述了试剂盒。

公开(公告)号：US20060276387A1

公开(公告)日：2006-12-07

申请号：US10544061

申请日：2004-11-26

申请人： Hirofumi Doi ,  Kensaku Imai ,  Naoya Wada

发明人： Hirofumi Doi ,  Kensaku Imai ,  Naoya Wada

IPC分类号： A61K38/22 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C07K14/62 ,  G01N33/74 ,  G01N2500/02

摘要： A method for promoting insulin gene transcription, which comprises the step of inhibiting binding of IPF1 and any one of proteins selected from the following group: (i) HNF3G, (ii) PHF1, and (iii) DLX4; and a method for screening a substance that promotes insulin gene transcription, which comprises the step of bringing a test substance into contact with IPF1 and/or any one of proteins selected from the following group under a condition that allows the binding of IPF1 and said protein and then determining whether or not the test substance inhibits the binding of IPF1 and said protein by detecting presence or absence, or change of a signal and/or a marker generated by the binding of IPF1 and said protein in a system in which the signal and/or the marker can be detected: (i) HNF3G, (ii) PHF1, and (iii) DLX4.

摘要翻译： 一种促进胰岛素基因转录的方法，其包括抑制IPF1与选自以下组中的任何一种蛋白质结合的步骤：（i）HNF3G，（ii）PHF1和（iii）DLX4; 以及用于筛选促进胰岛素基因转录的物质的方法，其包括在允许IPF1和所述蛋白质结合的条件下使测试物质与IPF1和/或选自以下组的任何一种蛋白质接触的步骤 然后通过检测存在或不存在检测物质来抑制IPF1和所述蛋白质的结合，或者通过IPF1和所述蛋白质的结合产生的信号和/或标记的变化来抑制IPF1和所述蛋白质的结合，其中信号和 /或可以检测标记：（i）HNF3G，（ii）PHF1和（iii）DLX4。

公开(公告)号：US20070105796A1

公开(公告)日：2007-05-10

申请号：US10563074

申请日：2005-04-28

申请人： Naoya Wada ,  Takashi Okamoto ,  Keiji Tanigaki ,  Hirofumi Doi ,  Yasuhiro Kikuchi ,  Kensaku Imai

发明人： Naoya Wada ,  Takashi Okamoto ,  Keiji Tanigaki ,  Hirofumi Doi ,  Yasuhiro Kikuchi ,  Kensaku Imai

IPC分类号： A61K48/00

CPC分类号： C12N9/1205

摘要： The present invention provides a method for inhibiting the activation of telomerase and an agent for inhibiting the activation of telomerase, a method for inhibiting telomerase activity and an agent for inhibiting telomerase activity, a method for preventing and/or a method for treating a cancer disease, an agent for preventing and/or an agent for treating a cancer disease, all of which comprises inhibiting the binding of MAPKAPK3 to TERT that is a catalytic subunit of telomerase or inhibiting the phosphorylation of TERT by active MAPKAPK3; a method of identifying a compound that inhibits the binding of MAPKAPK3 to TERT or a compound that inhibits the phosphorylation of TERT by active MAPKAPK3; and a reagent kit.

摘要翻译： 本发明提供抑制端粒酶活化的方法和抑制端粒酶活化的试剂，抑制端粒酶活性的方法和抑制端粒酶活性的方法，预防和/或治疗癌症的方法 ，用于预防和/或治疗癌症疾病的药物，所有这些都包括抑制MAPKAPK3与作为端粒酶催化亚基的TERT的结合或抑制活性MAPKAPK3的TERT磷酸化; 鉴定抑制MAPKAPK3与TERT结合的化合物或抑制活性MAPKAPK3磷酸化TERT的化合物的方法; 和试剂盒。

公开(公告)号：US08003346B2

公开(公告)日：2011-08-23

申请号：US11994437

申请日：2006-07-04

申请人： Tadaaki Tokida ,  Satoshi Hihara ,  Takashi Kudou ,  Akira Kawamura ,  Hirofumi Doi ,  Yoshizumi Ishino

发明人： Tadaaki Tokida ,  Satoshi Hihara ,  Takashi Kudou ,  Akira Kawamura ,  Hirofumi Doi ,  Yoshizumi Ishino

IPC分类号： G01N33/52 ,  C07H17/00 ,  C07K14/00

CPC分类号： C07K14/4738 ,  C12N9/1252 ,  C12N15/69

摘要： The present invention is to construct a DNA replication reaction system which is excellent in versatility and is easily used. An amino acid sequence of a PCNA monomer which is one of factors involved in DNA replication is prepared so that amino acid residues causing mutual charge repulsion constitute a site which causes, when an N terminal region of the PCNA monomer and a C terminal region of another PCNA monomer act as an interface to form a multimeric complex, an intermolecular interaction of the monomers in an interface region of the monomers.

摘要翻译： 本发明是构建通用性优异且易于使用的DNA复制反应体系。 制备作为涉及DNA复制的因素之一的PCNA单体的氨基酸序列，使得导致相互电荷排斥的氨基酸残基构成了当PCNA单体的N末端区域和另一个的C末端区域时引起的位点 PCNA单体作为形成多聚体复合物的界面，单体在界面区域中的分子间相互作用。

公开(公告)号：US20110183331A1

公开(公告)日：2011-07-28

申请号：US12993338

申请日：2009-05-19

申请人： Hirofumi Doi ,  Masahiro Matsuoka ,  Tatsuhiro Nakano ,  Chizuru Nagayoshi

发明人： Hirofumi Doi ,  Masahiro Matsuoka ,  Tatsuhiro Nakano ,  Chizuru Nagayoshi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6841 ,  C12Q2543/10 ,  C12Q2527/125 ,  C12Q2545/101

摘要： Disclosed is a method for identifying the presence of a target gene mRNA, which comprises hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes, wherein the oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA, and wherein the dummy oligonucleic acids are substantially equal in length with the oligonucleic acid probes, and are neither hybridizable with regions of the target gene mRNA with which the oligonucleic acid probes hybridize nor with the oligonucleic acid probes.

摘要翻译： 公开了一种鉴定目标基因mRNA的存在的方法，其包括将一种或多种寡核酸探针与组织样品中表达的靶基因mRNA杂交，并检测添加到以下的至少一种的低分子量化合物标记： 寡核酸探针的碱基，其中寡聚核酸探针与组织样品接触，用于与组织样品预处理（预杂交）（预杂交）一种或多种假寡聚核酸或寡核酸探针的混合物与靶基因mRNA杂交 使虚拟寡核酸与样品组织接触，将寡核酸探针与靶基因mRNA杂交，并且其中虚拟寡核酸与寡核酸探针的长度基本相等，并且不与靶基因的区域杂交 寡聚核酸探针与其杂交的mRNA也不与低聚核酸探针杂交。

公开(公告)号：US20100231070A1

公开(公告)日：2010-09-16

申请号：US12305872

申请日：2007-04-19

申请人： Kenta Hatano ,  Hirofumi Doi ,  Toshihiko Miyake

发明人： Kenta Hatano ,  Hirofumi Doi ,  Toshihiko Miyake

IPC分类号： H02K7/06

CPC分类号： H02K7/06

摘要： An actuator having an output axis which is moved linearly by a rotatory force, and a boss for holding the above-mentioned output axis in such a way that the output axis can move linearly includes a connecting member for propagating the linear motion of the above-mentioned output axis to an external device. The above-mentioned connecting member is comprised of a joint connected to the above-mentioned output axis, and a link plate having an end rotatably connected to the above-mentioned joint, and another end rotatably connected to the external device. A mechanism for preventing rotation of the above-mentioned output axis is constructed of only a connecting portion for connecting the above-mentioned joint, the above-mentioned link plate, and the above-mentioned external device to one another.

摘要翻译： 具有通过旋转力线性移动的输出轴的致动器和用于保持上述输出轴线的凸起，使得输出轴线性线性地移动，具有用于传播上述输出轴的直线运动的连接部件， 提到的输出轴到外部设备。 上述连接构件包括连接到上述输出轴线的接头和具有可旋转地连接到上述接头的端部的连接板，以及可旋转地连接到外部设备的另一端。 用于防止上述输出轴的旋转的机构仅由用于将上述接头，上述连接板和上述外部装置彼此连接的连接部分构成。

公开(公告)号：US20070134663A1

公开(公告)日：2007-06-14

申请号：US10568578

申请日：2004-08-13

申请人： Satoshi Hihara ,  Hirofumi Doi

发明人： Satoshi Hihara ,  Hirofumi Doi

IPC分类号： C12Q1/68 ,  C12Q1/48 ,  C12N9/99

CPC分类号： G01N33/6875 ,  C12Q1/485 ,  G01N2500/02

摘要： The task is to provide protein interaction inhibitors and/or detection method for interaction inhibitors related to regulation of T cell activation and production of IL-2. The present invention provides inhibitors of interaction between PKC theta and KPNA1, and inhibitors of interaction between KPNA1 and NF-kappaB. Inhibitors of interaction is defined as an candidate compound demonstrating the inhibition of interaction by assaying whether interaction has occurred or not between 2 types of protein and the candidate compound under the conditions allowing interaction of each combination. The detection kit is equipped with samples providing proteins of combinations to be interacted.

摘要翻译： 任务是提供蛋白质相互作用抑制剂和/或与调节T细胞活化和产生IL-2相关的相互作用抑制剂的检测方法。 本发明提供PKCθ与KPNA1之间相互作用的抑制剂，以及KPNA1与NF-κB之间相互作用的抑制剂。 相互作用的抑制剂被定义为通过测定在允许每种组合的相互作用的条件下，在两种类型的蛋白质和候选化合物之间是否发生相互作用来证明相互作用的抑制的候选化合物。 检测试剂盒配备提供待相互作用的组合蛋白质的样品。