公开(公告)号：US5115101A

公开(公告)日：1992-05-19

申请号：US721005

申请日：1991-06-26

申请人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

发明人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

IPC分类号： C07K16/06 ,  C07K16/36 ,  G01N33/68

CPC分类号： G01N33/6842 ,  C07K16/065 ,  C07K16/36 ,  G01N33/68

摘要： Antibody preparation purified using immobilized protein A and yet substantially free of protein A that may have solubilized during the purificaiton process. The antibodies include less than 15 ng protein A per mg of antibody, preferably less than 1 ng/mg. Low protein A content is obtained by first contacting the antibodies and solubilized protein A with an ion exchange resin under conditions to adsorb both. The antibodies and protein A are then sequentially eluted under conditions of increasing ionic strength.

摘要翻译： 使用固定化蛋白A纯化但基本上不含可能在纯化过程中溶解的蛋白A的抗体制备。 抗体包括每mg抗体少于15ng蛋白A，优选小于1ng / mg。 通过在吸附两者的条件下首先使抗体和溶解的蛋白A与离子交换树脂接触来获得低蛋白质A含量。 然后在增加离子强度的条件下将抗体和蛋白A顺序洗脱。

公开(公告)号：US4983722A

公开(公告)日：1991-01-08

申请号：US204054

申请日：1988-06-08

申请人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

发明人： James W. Bloom ,  Melvin F. Wong ,  Gautam Mitra

IPC分类号： G01N33/53 ,  A61K39/395 ,  C07K1/18 ,  C07K14/435 ,  C07K14/705 ,  C07K16/00 ,  C07K16/06 ,  C07K16/36 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  G01N33/531 ,  G01N33/68

CPC分类号： G01N33/6842 ,  C07K16/065 ,  C07K16/36 ,  G01N33/68

摘要： Protein A is selectively isolated from an antibody--Protein A mixture by exposing the mixture to an anion exchange material under conditions sufficient to adsorb both components and then sequentially eluting the antibodies and protein A under conditions of increasing ionic strength. Resulting antibody preparations have less than about 15 ng of Protein A per mg of antibody.

公开(公告)号：US4948877A

公开(公告)日：1990-08-14

申请号：US192044

申请日：1988-05-09

申请人： Gautam Mitra ,  Milton M. Mozen

发明人： Gautam Mitra ,  Milton M. Mozen

IPC分类号： A61K38/00 ,  C07K16/06

CPC分类号： C07K16/065 ,  A61K38/00

摘要： Immune serum globulins (ISG) can be made substantially free of infectious retroviruses by storing the ISG in a liquid state at conditions of pH, temperature and time sufficient to inactivate substantially all infectious retroviruses. Preferred inactivation methods involve use of either of two specified storage conditions: (1) at a pH equal to or less than about 4.25 at a temperature of about 27.degree. C. for at least 3 days, or (2) at a pH equal to or less than about 6.8 at a temperature of about 45.degree. C. for at least about 8 hours.

摘要翻译： 通过将pH值，温度和时间足以灭活基本上所有感染性逆转录病毒的条件，将ISG储存在液体状态，可以使免疫血清球蛋白（ISG）基本上不含感染性逆转录病毒。 优选的灭活方法包括使用两种特定的储存条件中的任一种：（1）在约27℃的温度下等于或小于约4.25的pH至少3天，或（2）在等于 或在约45℃的温度下小于约6.8，至少约8小时。

公开(公告)号：US4364861A

公开(公告)日：1982-12-21

申请号：US153341

申请日：1980-05-27

申请人： Gautam Mitra ,  Michael H. Coan ,  Shohachi Wada

发明人： Gautam Mitra ,  Michael H. Coan ,  Shohachi Wada

IPC分类号： A61K38/00 ,  C12N9/64 ,  A61K35/14 ,  A61K35/16 ,  A61K37/02 ,  C07G7/00

CPC分类号： C12N9/647 ,  A61K38/00 ,  Y10S530/831

摘要： Blood-coagulation-promoting products substantially free of thrombin are prepared from human blood plasma by contacting a human blood plasma fraction containing coagulation Factors II, VII, IX and X with an anion exchanger to adsorb the coagulation Factors, which are subsequently eluted from the anion exchanger. The eluate is treated to generate a substance having Factor VIII Inhibitor Bypassing Activity and being substantially free of thrombin, and activated Factor X.

摘要翻译： 通过将含有凝血因子II，VII，IX和X的人血浆级分与阴离子交换剂接触以吸附凝血因子，从人血浆中制备基本上不含凝血酶的促凝血产物，随后从阴离子 交换机。 处理洗脱液以产生具有因子VIII抑制剂旁路活性并且基本上不含凝血酶和活化因子X的物质。

公开(公告)号：US4496689A

公开(公告)日：1985-01-29

申请号：US565810

申请日：1983-12-27

申请人： Gautam Mitra

发明人： Gautam Mitra

IPC分类号： C12N11/02 ,  A61K35/14 ,  A61K35/16 ,  A61K38/00 ,  A61K38/16 ,  A61K38/55 ,  A61K47/48 ,  A61L2/00 ,  A61P11/00 ,  C07K1/22 ,  C07K14/81 ,  C07K17/02 ,  C07K17/06 ,  C07K19/00 ,  C07G7/00

CPC分类号： A61L2/0023 ,  A61K47/4823 ,  A61L2/0005 ,  C07K17/06 ,  A61K38/00 ,  Y10S514/826

摘要： There is disclosed a process for producing a covalently attached alpha-1-proteinase inhibitor - water soluble polymer complex useful for pulmonary emphysema therapy, a covalently attached alpha-1-proteinase inhibitor - water soluble polymer complex produced by the process, a composition thereof in a pharmaceutically acceptable carrier, and a method for treating pulmonary emphysema by administering to a human patient a therapeutically effective amount of the complex or preparation.

摘要翻译： 公开了一种共价连接的α-1-蛋白酶抑制剂 - 可用于肺气肿治疗的水溶性聚合物复合物，共价连接的α-1-蛋白酶抑制剂 - 通过该方法生产的水溶性聚合物复合物的方法，其组合物在 药学上可接受的载体，以及通过向人类患者施用治疗有效量的复合物或制剂来治疗肺气肿的方法。

公开(公告)号：US3972775A

公开(公告)日：1976-08-03

申请号：US484010

申请日：1974-06-28

申请人： Charles R. Wilke ,  Gautam Mitra

发明人： Charles R. Wilke ,  Gautam Mitra

IPC分类号： C12N9/42 ,  C12P19/14 ,  C13K1/02 ,  C12D13/02 ,  C12B3/08

CPC分类号： C12N9/2437 ,  C12P19/14 ,  C12Y302/01004 ,  C13K1/02 ,  Y10S435/813 ,  Y10S435/816 ,  Y10S435/929 ,  Y10S435/945

摘要： A process for the production of sugar, mainly glucose, by the enzymatic degradation of cellulosic materials, particularly cellulosic wastes, which comprises hydrolyzing the cellulosic material in the presence of cellulase enzyme to produce a sugar solution and recovering from the hydrolysis products a major proportion of the cellulase enzyme used in the hydrolysis reaction for re-use. At least a portion of the required makeup cellulase enzyme is produced in a two-stage operation wherein, in the first stage, a portion of the output sugar solution is utilized to grow a cellulase-secreting microorganism, and, in the second stage, cellulase enzyme formation is induced in the microorganism-containing culture medium by the addition of an appropriate inducer, such as a cellulosic material. Cellulase enzyme is precipitated from the culture liquid by the addition of an organic solvent material, such as a low molecular weight alkyl ketone or alcohol, and the cellulase precipitate is then fed to the hydrolysis reaction.

摘要翻译： 一种通过纤维素材料，特别是纤维素废物的酶降解来生产糖，主要是葡萄糖的方法，其包括在纤维素酶存在下水解纤维素材料以产生糖溶液并从水解产物中回收大部分 用于水解反应中的纤维素酶用于再利用。 至少一部分所需的补充纤维素酶在两阶段操作中产生，其中在第一阶段中，将一部分输出的糖溶液用于生长纤维素酶分泌微生物，并且在第二阶段中，纤维素酶 通过添加合适的诱导剂如纤维素材料在含微生物的培养基中诱导酶的形成。 纤维素酶通过加入有机溶剂材料如低分子量烷基酮或醇从培养液中沉淀出来，然后将纤维素酶沉淀物加入到水解反应中。

公开(公告)号：US07650027B2

公开(公告)日：2010-01-19

申请号：US11263534

申请日：2005-11-01

申请人： Murli Manohar Joshi ,  Ram Prakash Bajpai ,  Gautam Mitra ,  Harish Kumar Sardana ,  Hari Narayan Bhargaw ,  Saroj Batra

发明人： Murli Manohar Joshi ,  Ram Prakash Bajpai ,  Gautam Mitra ,  Harish Kumar Sardana ,  Hari Narayan Bhargaw ,  Saroj Batra

IPC分类号： G06K9/00 ,  B07C5/00 ,  G07D7/00

CPC分类号： G07D7/121 ,  G07D7/1205

摘要： A currency genuineness detection system using plurality of opto-electronic sensors with both transmission and reflective (including fluorescence) properties of security documents is developed. Both detection sensing strategies utilise integrated response of the wide optical band sensed under UV visible along with optional near infra red light illumination. A security document is examined under static condition. A window signal signature is thus possible from photodetectors responses for various kinds of documents of different denominations, kinds and country of origin. A programmable technique for checking the genuineness of a security document is possible by feeding a unique code of the currency under examination.

摘要翻译： 开发了使用多个安全文件传输和反射（包括荧光）特性的光电传感器的货币真实度检测系统。 两种检测传感策略都利用了在紫外可见下感测到的宽光谱带以及可选的近红外光照度的综合响应。 在静态条件下检查安全文件。 因此，可以对来自不同面额，种类和原籍国的各种文件的光电检测器响应进行窗口信号签名。 可以通过提供检查中的货币的唯一代码来检查安全文档的真实性的可编程技术。

公开(公告)号：US4543210A

公开(公告)日：1985-09-24

申请号：US658081

申请日：1984-10-04

申请人： Gautam Mitra ,  Paul K. Ng

发明人： Gautam Mitra ,  Paul K. Ng

IPC分类号： C07K14/745 ,  A61K35/14 ,  A61K35/16 ,  A61K38/00 ,  A61K38/16 ,  A61K38/43 ,  C07K14/435 ,  C07K14/755 ,  A23J1/06

CPC分类号： C07K14/755 ,  A61K38/00 ,  Y10S530/83

摘要： There is disclosed an improved process for producing high purity antihemophilic factor concentrate from an antihemophilic factor-containing dispersion or solution isolated from blood plasma or a blood plasma fraction, wherein the improvement is in carrying-out two consecutive precipitations using a combination of precipitants in each precipitation, first a combination of 1-4% by weight, based on weight of solution, of polyethylene glycol and 0.1-0.2 ml of 1-3%, based on weight of suspension, aluminum hydroxide suspension per gram of protein in the starting dispersion or solution, followed by a combination of added polyethylene glycol to provide a final concentration of 9-13% by weight, based on weight of the resulting solution, and 10-20% by weight of glycine, based on weight of the polyethylene glycol solution, and 10-20% by weight, based on weight of the polyethylene glycol solution, of sodium chloride.

摘要翻译： 公开了一种用于从分离自血浆或血浆级分的抗血友病因子分散体或溶液中产生高纯度抗血友病因子浓缩物的改进方法，其中改进是使用各自中的沉淀剂的组合进行两次连续的沉淀 沉淀，首先将1-4重量％的组合，基于溶液的重量，聚乙二醇和0.1-0.2ml的1-3％，基于悬浮液的重量，在起始分散体中每克蛋白质的氢氧化铝悬浮液 或溶液，随后加入的聚乙二醇的组合，以得到的溶液的重量为基准提供9-13重量％的终浓度，和基于聚乙二醇溶液重量的10-20重量％甘氨酸 ，10-20重量％，基于聚乙二醇溶液的重量计，氯化钠。

公开(公告)号：US4361510A

公开(公告)日：1982-11-30

申请号：US344912

申请日：1982-02-02

申请人： Gautam Mitra

发明人： Gautam Mitra

IPC分类号： A61K38/00 ,  C12N9/64 ,  A61K35/14 ,  A61K35/16 ,  C07G7/00

CPC分类号： C12N9/644 ,  C12N9/647 ,  C12Y304/21022 ,  A61K38/00 ,  Y10S530/831

摘要： The disclosure pertains to a concentrate for controlling bleeding in hemophilia, free of thrombin, heparin, thromboplastin activity, anticomplement activity, depressor activity, and activated Factor X, and containing coagulation Factors II, VII, IX, and X in non-activated form, having a specific activity of at least about 1.5 Factor IX units per milligram of total protein and a Factor IX: Factor VII ratio of at least about 6:1. In the method of the invention a blood fraction containing coagulation Factors II, VII, IX, and X is applied to an anion exchanger to adsorb the coagulation Factors, which are selectively eluted therefrom using an aqueous sodium chloride-citrate solution of increasing ionic strength. The eluate is treated to reduce its concentration of non-volatile salt and concentrated to yield the above product.

摘要翻译： 本公开涉及用于控制血友病出血的浓缩物，其不含凝血酶，肝素，促凝血酶原激活素活性，抗补体活性，抑制剂活性和活化因子X，并且含有非活化形式的凝血因子II，VII，IX和X， 具有至少约1.5倍因子IX单位/毫克总蛋白和因子IX：因子VII比例至少约6:1的比活性。 在本发明的方法中，将含有凝血因子II，VII，IX和X的血液部分施用于阴离子交换剂以吸附凝血因子，其使用增加离子强度的氯化钠 - 柠檬酸钠水溶液选择性地从其中洗脱。 处理洗脱液以减少其非挥发性盐的浓度并浓缩以产生上述产物。

公开(公告)号：US07912272B2

公开(公告)日：2011-03-22

申请号：US12648176

申请日：2009-12-28

申请人： Murli Manohar Joshi ,  Ram Prakash Bajpai ,  Gautam Mitra ,  Harish Kumar Sardana ,  Hari Narayan Bhargaw ,  Saroj Batra

发明人： Murli Manohar Joshi ,  Ram Prakash Bajpai ,  Gautam Mitra ,  Harish Kumar Sardana ,  Hari Narayan Bhargaw ,  Saroj Batra

IPC分类号： G06K9/00 ,  G06K7/00 ,  G06K9/74 ,  B07C5/00

CPC分类号： G07D7/121 ,  G07D7/1205

摘要： A currency genuineness detection system using plurality of opto-electronic sensors with both transmission and reflective (including fluorescence) properties of security documents is developed. Both detection sensing strategies utilize integrated response of the wide optical band sensed under UV visible along with optional near infra red light illumination. A security document is examined under static condition. A window signal signature is thus possible from photodetectors responses for various kinds of documents of different denominations, kinds and country of origin. A programmable technique for checking the genuineness of a security document is possible by feeding a unique code of the currency under examination.

摘要翻译： 开发了使用多个安全文件传输和反射（包括荧光）特性的光电传感器的货币真实度检测系统。 两种检测传感策略都利用了在紫外可见下感测到的宽光谱带以及可选的近红外光照度的综合响应。 在静态条件下检查安全文件。 因此，可以对来自不同面额，种类和原籍国的各种文件的光电检测器响应进行窗口信号签名。 可以通过提供检查中的货币的唯一代码来检查安全文档的真实性的可编程技术。