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    BIOTECHNOLOGICAL PREPARATION OF 3-HYDROXYISOBUTYRIC ACID
    2.
    发明申请
    BIOTECHNOLOGICAL PREPARATION OF 3-HYDROXYISOBUTYRIC ACID 审中-公开
    3-羟基丁酸的生物技术制备

    公开(公告)号：US20150218600A1

    公开(公告)日：2015-08-06

    申请号：US14363178

    申请日：2012-11-14

    申请人： Thomas Haas ,  Steffen Schaffer ,  Markus Poetter ,  Mirja Wessel ,  Jan Christoph Pfeffer ,  Christian Gehring ,  Nicole Kirchner ,  Eva Maria Wittmann

    发明人： Thomas Haas ,  Steffen Schaffer ,  Markus Poetter ,  Mirja Wessel ,  Jan Christoph Pfeffer ,  Christian Gehring ,  Nicole Kirchner ,  Eva Maria Wittmann

    IPC分类号： C12P7/42 ,  C12N9/88 ,  C12N9/12 ,  C12N9/02 ,  C12N9/00 ,  C12N9/10

    CPC分类号： C12P7/42 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/1217 ,  C12N9/13 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12Y103/99002 ,  C12Y207/02007 ,  C12Y402/01017 ,  C12Y602/01002

    摘要： The invention relates to a method comprising the steps a) providing isobutyric acid, b) bringing isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A synthetase/ligase and/or isobutyrate-coenzyme A transferase, c) bringing the product from step a) into contact with isobutyryl-coenzyme A dehydrogenase, d) bringing the product from step b) into contact with methacrylyl-coenzyme A hydratase, and e) hydrolyzing the product from step d) to form 3-hydroxyisobutyric acid, where at least one of the enzymes is used in the form of a cell which, compared to its wildtype, comprises a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, a cell which has at least one enzyme from the group comprising isobutyryl-coenzyme A synthetase/ligase, isobutyrate-coenzyme A transferase, isobutyrate kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A dehydrogenase, methacrylyl-coenzyme A hydratase and 3-hydroxyisobutyryl-coenzyme A hydrolase and, compared to its wildtype, a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, wherein the cell preferably has, in addition, a monooxygenase, more preferably a monooxygenase of the alkBGT type or a variant thereof and the use of such a cell for preparing 3-hydroxyisobutyric acid.

    摘要翻译： 本发明涉及包括以下步骤的方法：a）提供异丁酸，b）使异丁酸与异丁酸酯激酶和磷酸转移异丁酰基酶和/或异丁酰辅酶A合成酶/连接酶和/或异丁酸酯辅酶A转移酶c的组合接触 ）使来自步骤a）的产物与异丁酰辅酶A脱氢酶接触，d）将来自步骤b）的产物与甲基丙烯酰辅酶A水合酶接触，和e）水解步骤d）中的产物以形成3-羟基异丁酸 酸，其中至少一种酶以细胞形式使用，与其野生型相比，其包含3-羟基异丁酸脱氢酶或其变体的降低的活性，具有至少一种酶的细胞 异丁酰辅酶A合成酶/连接酶，异丁酸酯辅酶A转移酶，异丁酸酯激酶，磷酸转移异丁酰基酶，异丁酰辅酶A脱氢酶，甲基丙烯酰辅酶A水合酶和 3-羟基异丁酰辅酶A水解酶，与其野生型相比，3-羟基异丁酸脱氢酶或其变体的活性降低，其中细胞优选还具有单加氧酶，更优选alkBGT型的单加氧酶或 其变体以及这种细胞用于制备3-羟基异丁酸的用途。

    METHOD FOR THE PRODUCTION OF FREE CARBOXYLIC ACIDS
    3.
    发明申请
    METHOD FOR THE PRODUCTION OF FREE CARBOXYLIC ACIDS 失效
    生产游离羧酸的方法

    公开(公告)号：US20110189742A1

    公开(公告)日：2011-08-04

    申请号：US13002519

    申请日：2009-04-29

    申请人： Thomas Haas ,  Thomas Tacke ,  Achim Marx ,  Alexander Schraven ,  Olivier Zehnacker ,  Eva Maria Wittmann

    发明人： Thomas Haas ,  Thomas Tacke ,  Achim Marx ,  Alexander Schraven ,  Olivier Zehnacker ,  Eva Maria Wittmann

    IPC分类号： C12P7/40

    CPC分类号： C12P7/40 ,  C07C51/02 ,  C07C51/41 ,  C07C51/47 ,  C12P7/42 ,  C07C59/01 ,  C07C59/06 ,  C07C59/255 ,  C07C59/08 ,  C07C59/265

    摘要： A process for the preparation of free carboxylic acids including: A) preparation of carboxylic acid by a biological cell located in an aqueous medium with addition of an amine of formula (I) where R1, R2 and R3, independently of one another, are identical or different, branched or unbranched, optionally substituted hydrocarbon radicals or H; B) for cases where the added amine A) is water-soluble, addition of a water-insoluble amine of formula (I), where, in A) or B), a multiphase system is obtained and the corresponding ammonium carboxylate is formed from the water-insoluble amine and the carboxylic acid; C) removal of the water-insoluble phase; and D) heating of the water-insoluble phase with release of free carboxylic acid.

    摘要翻译： 一种制备游离羧酸的方法，包括：A）通过加入式（I）的胺的位于水性介质中的生物细胞制备羧酸，其中R1，R2和R3彼此独立地相同 或不同的支链或非支链的任选取代的烃基或H; B）对于添加的胺A）是水溶性的，加入式（I）的水不溶性胺，其中在A）或B）中获得多相体系，并且相应的羧酸铵由 水不溶性胺和羧酸; C）去除水不溶性相; 和D）释放游离羧酸时加热水不溶性相。

    MULTI-STAGE SYNTHESIS METHOD WITH SYNTHESIS GAS
    4.
    发明申请
    MULTI-STAGE SYNTHESIS METHOD WITH SYNTHESIS GAS 审中-公开
    具有合成气的多阶段综合方法

    公开(公告)号：US20150125912A1

    公开(公告)日：2015-05-07

    申请号：US14400379

    申请日：2013-05-08

    申请人： Thomas Haas ,  Eva Maria Wittmann

    发明人： Thomas Haas ,  Eva Maria Wittmann

    IPC分类号： C12P7/64 ,  C12P7/40 ,  C12P7/62 ,  C12P7/42

    摘要： The invention provides a process for the preparation of hydrocarbons substituted with at least one group containing at least one oxygen atom, comprising the process steps A) reaction of a carbon source comprising at least one selected from CO2 and CO to give acetate and/or ethanol with a first microorganism, B) separating off of the acetate from the first microorganism, C) reaction of the acetate to give a hydrocarbon substituted with at least one group containing at least one oxygen atom with a second microorganism and optionally D) purification of the hydrocarbon substituted with at least one group containing at least one oxygen atom.

    摘要翻译： 本发明提供一种制备被至少一个含有至少一个氧原子的基团取代的烃的方法，包括以下工艺步骤：A）包含至少一种选自CO 2和CO的碳源的反应，得到乙酸酯和/或乙醇 与第一微生物B）从第一微生物分离出乙酸酯，C）乙酸酯的反应，得到被至少一个含有至少一个氧原子的基团取代的烃与第二微生物的反应，任选地D）纯化 被至少一个含有至少一个氧原子的基团取代的烃。