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    METHOD FOR THE PRODUCTION OF FREE CARBOXYLIC ACIDS
    1.
    发明申请
    METHOD FOR THE PRODUCTION OF FREE CARBOXYLIC ACIDS 失效
    生产游离羧酸的方法

    公开(公告)号：US20110189742A1

    公开(公告)日：2011-08-04

    申请号：US13002519

    申请日：2009-04-29

    申请人： Thomas Haas ,  Thomas Tacke ,  Achim Marx ,  Alexander Schraven ,  Olivier Zehnacker ,  Eva Maria Wittmann

    发明人： Thomas Haas ,  Thomas Tacke ,  Achim Marx ,  Alexander Schraven ,  Olivier Zehnacker ,  Eva Maria Wittmann

    IPC分类号： C12P7/40

    CPC分类号： C12P7/40 ,  C07C51/02 ,  C07C51/41 ,  C07C51/47 ,  C12P7/42 ,  C07C59/01 ,  C07C59/06 ,  C07C59/255 ,  C07C59/08 ,  C07C59/265

    摘要： A process for the preparation of free carboxylic acids including: A) preparation of carboxylic acid by a biological cell located in an aqueous medium with addition of an amine of formula (I) where R1, R2 and R3, independently of one another, are identical or different, branched or unbranched, optionally substituted hydrocarbon radicals or H; B) for cases where the added amine A) is water-soluble, addition of a water-insoluble amine of formula (I), where, in A) or B), a multiphase system is obtained and the corresponding ammonium carboxylate is formed from the water-insoluble amine and the carboxylic acid; C) removal of the water-insoluble phase; and D) heating of the water-insoluble phase with release of free carboxylic acid.

    摘要翻译： 一种制备游离羧酸的方法，包括：A）通过加入式（I）的胺的位于水性介质中的生物细胞制备羧酸，其中R1，R2和R3彼此独立地相同 或不同的支链或非支链的任选取代的烃基或H; B）对于添加的胺A）是水溶性的，加入式（I）的水不溶性胺，其中在A）或B）中获得多相体系，并且相应的羧酸铵由 水不溶性胺和羧酸; C）去除水不溶性相; 和D）释放游离羧酸时加热水不溶性相。

    RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID
    4.
    发明申请
    RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID 审中-公开
    重组细胞生产2-羟基丁酸

    公开(公告)号：US20110171702A1

    公开(公告)日：2011-07-14

    申请号：US13001204

    申请日：2009-04-28

    申请人： Liv Reinecke ,  Steffen Schaffer ,  Achim Marx ,  Markus Poetter ,  Thomas Haas

    发明人： Liv Reinecke ,  Steffen Schaffer ,  Achim Marx ,  Markus Poetter ,  Thomas Haas

    IPC分类号： C12P7/40 ,  C12N5/10 ,  C12N1/00 ,  C12P7/42 ,  C12P7/62

    CPC分类号： C12P7/625 ,  C12P7/42

    摘要： The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor.

    摘要翻译： 本发明涉及一种经遗传修饰以能够产生比其野生型更多的2-羟基异丁酸或更多含2-羟基异丁酸单体单元的聚羟基链烷酸酯的细胞，其特征在于2-羟基异丁酸或含有2-羟基异丁酸的聚羟基链烷酸酯， 通过作为中间体的乙酰乙酰辅酶A和作为前体的3-羟基丁酰辅酶A制备羟基异丁酸单体单元。

    Process for preparing methacrylic acid or methacrylic esters
    5.
    发明授权
    Process for preparing methacrylic acid or methacrylic esters 有权
    制备甲基丙烯酸或甲基丙烯酸酯的方法

    公开(公告)号：US09234218B2

    公开(公告)日：2016-01-12

    申请号：US12602593

    申请日：2008-05-30

    申请人： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

    发明人： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

    IPC分类号： C12P7/42 ,  C12P7/52 ,  C12N9/02 ,  C12P7/40 ,  C12P7/62 ,  C08F120/06 ,  C08F120/10 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88

    CPC分类号： C12P7/42 ,  C08F120/06 ,  C08F120/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/52 ,  C12P7/625 ,  C12Y102/04004 ,  C12Y103/99012 ,  C12Y206/01042 ,  C12Y301/02004 ,  C12Y402/01017

    摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.

    摘要翻译： 本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其 野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳 二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸， 如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸 酸，并且在适当的情况下酯化甲基丙烯酸。 本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。

    Nucleotide sequences which code for the menE gene
    7.
    发明授权
    Nucleotide sequences which code for the menE gene 失效
    编码menE基因的核苷酸序列

    公开(公告)号：US06946271B2

    公开(公告)日：2005-09-20

    申请号：US09834722

    申请日：2001-04-16

    申请人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Walter Pfefferle

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12N9/00 ,  C12P13/08

    CPC分类号： C12N9/93 ,  C12P13/08 ,  C12Y602/01026

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the menE gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含SEQ ID NO： 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与 ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少所述menE 基因以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

    Nucleotide sequences encoding histidine kinase from corynebacterium glutamicum
    8.
    发明授权
    Nucleotide sequences encoding histidine kinase from corynebacterium glutamicum 失效
    编码来自谷氨酸棒杆菌的组氨酸激酶的核苷酸序列

    公开(公告)号：US06746854B2

    公开(公告)日：2004-06-08

    申请号：US09824551

    申请日：2001-08-01

    申请人： Brigitte Bathe ,  Caroline Kreutzer ,  Achim Marx ,  Walter Pfefferle

    发明人： Brigitte Bathe ,  Caroline Kreutzer ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12P1308

    CPC分类号： C12N9/1223 ,  C12P13/08

    摘要： This invention relates to novel polynucleotide sequences encoding the histidine kinase luminescence expression sensor (luxS) gene from Corynebaclerium glutamicum, probes to the novel polynucleotide sequences encoding the luxS gene, vector and host cells containing the novel luxS polynucleotide sequences, the encoded Lux S polypeptide, and a process for the fermentative preparation of amino acids using bacteria which the luxS gene is attenuated.

    摘要翻译： 本发明涉及编码来自谷氨酸棒杆菌的组氨酸激酶发光表达传感器（luxS）基因的新型多核苷酸序列，探针编码luxS基因的新型多核苷酸序列，含有新型luxS多核苷酸序列的载体和宿主细胞，编码的Lux S多肽， 以及使用luxS基因减毒的细菌发酵制备氨基酸的方法。

    Nucleotide sequences which code for the zwa1 gene
    9.
    发明授权
    Nucleotide sequences which code for the zwa1 gene 失效
    编码zwa1基因的核苷酸序列

    公开(公告)号：US06632644B2

    公开(公告)日：2003-10-14

    申请号：US09731909

    申请日：2000-12-08

    申请人： Bettina Möckel ,  Walter Pfefferle ,  Achim Marx ,  Jörn Kalinowski ,  Brigitte Bathe ,  Alfred Pühler

    发明人： Bettina Möckel ,  Walter Pfefferle ,  Achim Marx ,  Jörn Kalinowski ,  Brigitte Bathe ,  Alfred Pühler

    IPC分类号： C12P1308

    CPC分类号： C07K14/34 ,  C12N9/00 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.

    摘要翻译： 本发明涉及一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含氨基酸序列 b）编码多肽的多核苷酸，其包含与SEQ ID NO 2的氨基酸序列至少70％的程度相同的氨基酸序列，c） 与a）和b）和d）多核苷酸互补的多核苷酸，其包含a），b）或c），/或PTEXT的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及发酵 在使用棒状细菌中扩增zwa1基因的L-氨基酸的制备