-
公开(公告)号：US07585674B2
公开(公告)日：2009-09-08
申请号：US10479214
申请日：2002-05-28
申请人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
发明人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
CPC分类号： C07K14/32 , C07K2319/00 , C12N1/20
摘要： A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.
摘要翻译： 一种微生物，其中一个或多个选自参与孢子形成中期至晚期孢子形成的基因的基因已被删除或失活; 以及通过使用微生物生产目标产物（蛋白质）的方法。 当使用该微生物时不形成孢子，从而能够减少能量损失，副产物的产生和特定的生产速度，从而能够生产目标产物（蛋白质），从而减少不必要的培养基消耗。 此外，可以延长生产周期，由此有效地生产目标产物（蛋白质）。
-
公开(公告)号：US07981659B2
公开(公告)日：2011-07-19
申请号：US12083539
申请日：2006-09-25
IPC分类号： C12N1/20
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。
-
公开(公告)号：US08778650B2
公开(公告)日：2014-07-15
申请号：US13266525
申请日：2010-04-26
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： The invention is directed to an alkaline protease that has the amino acid sequence of SEQ ID NO: 2 or of an amino acid sequence having an identity of 90% or more thereto, except that one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of SEQ ID NO: 2, or at positions corresponding thereto, are substituted with certain other amino acid residues. The invention is also directed to detergent compositions and culture medium that contain the protease.
摘要翻译： 本发明涉及具有SEQ ID NO：2的氨基酸序列或90％以上的氨基酸序列的碱性蛋白酶，除了选自（a）的位置的一个或多个氨基酸残基 ）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h）位置204，（i） 319和（j）SEQ ID NO：2的位置337或与其相应的位置被某些其它氨基酸残基取代。 本发明还涉及含有蛋白酶的洗涤剂组合物和培养基。
-
公开(公告)号：US20120058928A1
公开(公告)日：2012-03-08
申请号：US13266525
申请日：2010-04-26
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, H is, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, H is, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
摘要翻译： 由碱性蛋白酶衍生的碱性蛋白酶变体，其由SEQ ID NO：2所示的氨基酸序列组成或由与其同一性为90％以上的氨基酸序列组成，所述变体具有突变，其中一个或多个氨基酸残基 在从（a）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h） 204，（i）位置319和（j）由SEQ ID NO：2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代：（a）或与其对应的位置： Typ，Leu，Val，Ile，Met，Tyr，Gln，Lys，Thr，Phe，Arg，Ser，Cys，Ala或His; （b）或相应位置：Glu，Met，Asp，Val，Gln，Arg，Cys，Trp，Ala或Phe; （c）或对应的位置：Met，Glu，Arg，Val，Lys，Phe，Tyr，Ile，His，Asp或Cys; （d）或与之对应的位置：Trp; （e）或与之对应的位置：His，Trp，Ser或Leu; （f）或对应位置：Ala，Glu，Gln，Ser，Cys，Gly，H is，Lys，Arg，Met或Asn; （g）或对应的位置：Ala，Ser或Cys; （h）或相应位置：Glu，Asp，Cys，Val，Thr，Pro，His，Ile，Trp，Ser，Asn，Lys或Arg; （i）或与其相对应的位置：Trp，Val，Thr，Leu，Ile，Cys，Glu，Lys，Tyr，Arg，Phe，Gln，Met，Pro，Asp，Asn，H is或Ser; 和（j）或对应的位置：Arg，Gly，Ser，Lys，Gln，Thr，His，Ala，Cys或Val。
-
公开(公告)号：US20090221055A1
公开(公告)日：2009-09-03
申请号：US12083539
申请日：2006-09-25
IPC分类号： C12N1/21
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。
-
公开(公告)号：US07829322B2
公开(公告)日：2010-11-09
申请号：US11722162
申请日：2005-12-20
申请人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
发明人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
摘要： A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.
摘要翻译： 提供能够提高蛋白质或多肽的生产力的宿主微生物，通过将编码蛋白质或多肽的基因导入宿主微生物而获得的重组微生物，以及使用该重组微生物产生蛋白质或多肽的方法。 还提供了通过向宿主微生物中导入编码异源蛋白质或多肽的基因而获得的重组微生物，其中在所述宿主微生物中，枯草芽孢杆菌aprX基因或与aprX基因相对应的基因已被缺失或敲除， 用于使用重组微生物产生蛋白质或多肽。
-
公开(公告)号：US20090170154A1
公开(公告)日：2009-07-02
申请号：US10590275
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
CPC分类号： C12N9/2417 , C12N9/2437 , C12N9/54 , C12Y302/01004
摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.
摘要翻译： 本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。 一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物 通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。
-
公开(公告)号：US20080026976A1
公开(公告)日：2008-01-31
申请号：US11590769
申请日：2006-11-01
申请人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
发明人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to mutant α-amylases that may be produced at high yield from recombinant microorganisms.
摘要翻译： 本发明涉及可以从重组微生物以高产率产生的突变型α-淀粉酶。
-
公开(公告)号：US07033981B2
公开(公告)日：2006-04-25
申请号：US10383630
申请日：2003-03-10
申请人： Yoshihiro Hakamada , Kazuhisa Sawada , Keiji Endo , Hiroshi Kodama , Yasunao Wada , Shitsu Shikata , Tohru Kobayashi
发明人： Yoshihiro Hakamada , Kazuhisa Sawada , Keiji Endo , Hiroshi Kodama , Yasunao Wada , Shitsu Shikata , Tohru Kobayashi
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004
摘要： The present invention relates to alkaline cellulase variants having an amino acid sequence which exhibits at least 95% homology with the amino acid sequence of SEQ ID NO: 2, wherein said alkaline cellulase variant is mutated to include at least one substitution at (a) position 10, (b) position 16, (c) position 22, (d) position 33, (e) position 39, (f) position 76, (g) position 109, (h) position 242, (i) position 263, (j) position 308, (k) position 462, (l) position 466, (m) position 468, (n) position 552, (o) position 564, or (p) position 608 in SEQ ID NO: 2, or at a position corresponding thereto, wherein said alkaline cellulase variant has alkaline cellulase activity. The present invention also relates to a gene encoding the variant; a vector containing the gene; a transformant containing the vector; and a detergent composition containing the alkaline cellulase variant.
摘要翻译： 本发明涉及具有与SEQ ID NO：2的氨基酸序列具有至少95％同源性的氨基酸序列的碱性纤维素酶变体，其中所述碱性纤维素酶变体突变以在（a）位置包括至少一个取代 10，（b）位置16，（c）位置22，（d）位置33，（e）位置39，（f）位置76，（g）位置109，（h）位置242，（i）位置263， （j）位置308，（k）SEQ ID NO：2中的位置462，（l）位置466，（m）位置468，（n）位置552，（o）位置564或（p）位置608，或 在相应的位置，其中所述碱性纤维素酶变体具有碱性纤维素酶活性。 本发明还涉及编码该变体的基因; 含有该基因的载体; 含有载体的转化体 和含有碱性纤维素酶变体的洗涤剂组合物。
-
公开(公告)号：US07855065B2
公开(公告)日：2010-12-21
申请号：US10590275
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
CPC分类号： C12N9/2417 , C12N9/2437 , C12N9/54 , C12Y302/01004
摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism. A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.
摘要翻译： 本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。 一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物 通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。
-
-
-
-
-
-
-
-
-
搜索结果
88 条记录 (耗时 0.024 秒)