公开(公告)号：US07151170B1

公开(公告)日：2006-12-19

申请号：US09980364

申请日：2000-06-02

申请人： Kim Boutilier ,  Therese Ouellet ,  Jan Custers ,  Jiro Hattori ,  Brian Miki ,  Michiel Van Lookeren Campagne

发明人： Kim Boutilier ,  Therese Ouellet ,  Jan Custers ,  Jiro Hattori ,  Brian Miki ,  Michiel Van Lookeren Campagne

IPC分类号： C12N15/29

CPC分类号： C12N15/8261 ,  C12N15/8209 ,  C12N15/8231 ,  Y02A40/146

摘要： The present invention provides for a gene obtained during the induction of microspore embryogenisis. The protein encoded by this gene renders plant cells embryongenic, and increases the regenerative capacity of the plant cell. Also disclosed is the regulatory region of this gene and its use for directing the expression of a gene of interest within a suitable host cell.

摘要翻译： 本发明提供了在诱导小孢子胚发生过程中获得的基因。 由该基因编码的蛋白质使植物细胞胚胎发育，并增加植物细胞的再生能力。 还公开了该基因的调节区及其用于在合适的宿主细胞内引导感兴趣的基因的表达的用途。

公开(公告)号：US20050055742A1

公开(公告)日：2005-03-10

申请号：US10866529

申请日：2004-06-10

申请人： Brian Miki ,  Jiro Hattori ,  Teresa Martin-Heller ,  Helene Labbe ,  Kamal Malik ,  Elizabeth Foster ,  Keqiang Wu ,  Daniel Brown ,  Lining Tian ,  Therese Ouellet ,  Peijun Zhang ,  Elizabeth James ,  Pierre Fobert ,  Venkatram Iyer

发明人： Brian Miki ,  Jiro Hattori ,  Teresa Martin-Heller ,  Helene Labbe ,  Kamal Malik ,  Elizabeth Foster ,  Keqiang Wu ,  Daniel Brown ,  Lining Tian ,  Therese Ouellet ,  Peijun Zhang ,  Elizabeth James ,  Pierre Fobert ,  Venkatram Iyer

IPC分类号： C07K14/415 ,  C12N15/82 ,  C12Q1/68 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04

CPC分类号： C07K14/415 ,  C12N15/8216

摘要： An nucleotide sequence and that exhibits regulatory element activity is disclosed. The nucleotide sequence may be defined by SEQ ID NO:22, a nucleotide sequence that hybridizes to the nucleic acid sequence of SEQ ID NO:22, or a compliment thereof. Also disclosed is a chimeric construct comprising the nucleotide sequence operatively linked with a coding region of interest. A method of expressing a coding region of interest within a plant by introducing the chimeric construct described above, into the plant, and expressing the coding region of interest is also provided. Also disclosed are plants, seed, or plant cells comprising the chimeric construct as defined above.

摘要翻译： 公开了核苷酸序列并表现出调节元件活性。 核苷酸序列可以由SEQ ID NO：22定义，与SEQ ID NO：22的核酸序列杂交的核苷酸序列或其补体。 还公开了包含与感兴趣的编码区可操作地连接的核苷酸序列的嵌合构建体。 还提供了通过将上述嵌合构建体引入植物并表达感兴趣的编码区而在植物内表达感兴趣的编码区的方法。 还公开了包含如上定义的嵌合构建体的植物，种子或植物细胞。

公开(公告)号：US5824872A

公开(公告)日：1998-10-20

申请号：US593121

申请日：1996-02-01

申请人： Brian Miki ,  Jiro Hattori ,  Pierre Fobert ,  Venkatran N. Iyer

发明人： Brian Miki ,  Jiro Hattori ,  Pierre Fobert ,  Venkatran N. Iyer

IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H5/00 ,  C07H21/04 ,  C12N15/63

CPC分类号： C07K14/415 ,  C12N15/8216

摘要： T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity constitutively. The gene fusion has been cloned and sequenced. It has been re-inserted into N. tabacum by Agrobacterium-mediated transformation. The N. tabacum DNA upstream from the GUS gene was approximately 2 kb in length and showed no homology to known sequences. This DNA, which contains a constitutive promoter, is useful in controlling the expression of exogenous genes in transgenic plants of diverse plant species.

摘要翻译： 用无启动子β-葡糖醛酸糖苷酶（GUS）基因的T-DNA标签产生了组成型表达GUS活性的转基因烟草植物。 基因融合已被克隆并测序。 通过农杆菌介导的转化将其重新插入到烟草中。 GUS基因上游的烟草DNA长度约为2 kb，与已知序列无同源性。 含有组成型启动子的该DNA可用于控制不同植物物种的转基因植物中外源基因的表达。

公开(公告)号：US07303873B2

公开(公告)日：2007-12-04

申请号：US10437261

申请日：2003-05-13

申请人： Brian Miki ,  Thérèse Ouellet ,  Jiro Hattori ,  Elizabeth Foster ,  Hélène Labbé ,  Teresa Martin-Heller ,  Lining Tian ,  Daniel Charles William Brown ,  Peijun Zhang ,  Keqiang Wu

发明人： Brian Miki ,  Thérèse Ouellet ,  Jiro Hattori ,  Elizabeth Foster ,  Hélène Labbé ,  Teresa Martin-Heller ,  Lining Tian ,  Daniel Charles William Brown ,  Peijun Zhang ,  Keqiang Wu

IPC分类号： C12Q1/68 ,  C12N15/63 ,  C12N15/82 ,  C07H21/02 ,  C12N5/04

CPC分类号： C07K14/415 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8234

摘要： T-DNA tagging with a promoterless β-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.

摘要翻译： 用无启动子β-葡糖醛酸糖苷酶（GUS）基因的T-DNA标签产生转基因烟草植物，其仅在开发种皮或组成型中表达GUS活性。 GUS融合物的克隆和缺失分析表明，负责种衣特异性的启动子位于植物DNA接近GUS基因的位置。 该区域的分析表明，该转基因植物中GUS表达的种皮特异性是由隐匿启动子旁边的T-DNA插入产生的。 该启动子可用于控制植物种子中发育中的种皮的基因表达。 类似地，隐匿性组成型启动子的克隆和表征揭示了几个隐蔽调节区的发生。 这些区域包括启动子，负调控元件，转录增强子，核心启动子区和翻译增强子等调控元件。

公开(公告)号：US5756324A

公开(公告)日：1998-05-26

申请号：US625198

申请日：1996-04-01

申请人： Chris Baszczynski ,  Eric Barbour ,  Brian Miki ,  Jiro Hattori

发明人： Chris Baszczynski ,  Eric Barbour ,  Brian Miki ,  Jiro Hattori

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  C12N15/32 ,  C12N15/33

CPC分类号： C07K14/415 ,  C12N15/8222 ,  C12N15/8286 ,  C12N15/8289

摘要： A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.

摘要翻译： 已经发现，分离和表征赋予小孢子特异性基因表达的新型DNA调节元件。 小孢子特异性调节元件可用于控制破坏小孢子功能的外源基因的表达。 因此，可以通过使用小孢子特异性调节元件来产生雄性不育植物来实现对花粉产生的控制。 可以使用各种方法来恢复F1代这种雄性不育植物中的雄性生育力。 此外，小孢子特异性调节元件可用于赋予对病毒和昆虫害虫的抗性。

公开(公告)号：US5633438A

公开(公告)日：1997-05-27

申请号：US345756

申请日：1994-11-22

申请人： Chris Baszczynski ,  Eric Barbour ,  Brian Miki ,  Jiro Hattori

发明人： Chris Baszczynski ,  Eric Barbour ,  Brian Miki ,  Jiro Hattori

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H1/02

CPC分类号： C07K14/415 ,  C12N15/8222 ,  C12N15/8286 ,  C12N15/8289

摘要： A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.

摘要翻译： 已经发现，分离和表征赋予小孢子特异性基因表达的新型DNA调节元件。 小孢子特异性调节元件可用于控制破坏小孢子功能的外源基因的表达。 因此，可以通过使用小孢子特异性调节元件来产生雄性不育植物来实现对花粉产生的控制。 可以使用各种方法来恢复F1代这种雄性不育植物中的雄性生育力。 此外，小孢子特异性调节元件可用于赋予对病毒和昆虫害虫的抗性。

公开(公告)号：US20110059384A1

公开(公告)日：2011-03-10

申请号：US12991279

申请日：2009-04-14

申请人： Yukihisa Okada ,  Hideyuki Okada ,  Jiro Hattori

发明人： Yukihisa Okada ,  Hideyuki Okada ,  Jiro Hattori

IPC分类号： H01M8/10 ,  H01M8/00

CPC分类号： H01M8/1016 ,  H01M8/04223 ,  H01M2008/1095 ,  Y02P70/56

摘要： In the conventional initial operation and activation processing (pre-processing), a processing time of ten odd hours or more is usually required, and special processing equipment and complex processing steps are needed. An aqueous alcohol solution is prepared, a membrane electrode assembly (10) for a solid polymer-type fuel cell is brought into contact with the aqueous alcohol solution, and the assembly (10) is then washed with water. Then, the membrane electrode assembly (10) is sandwiched between bipolar plates (30, 31) to configure a unit cell. The unit cell is sandwiched between collector plates (50, 51), a plurality of unit cells sandwiched between the collector plates are stacked, and the stack is tightened and held between insulating plates (60, 61) and end plates (70, 71) to produce a solid polymer-type fuel cell.

摘要翻译： 在常规的初始操作和激活处理（预处理）中，通常需要十个小时以上的处理时间，并且需要特殊的处理设备和复杂的处理步骤。 制备含水醇溶液，使固体聚合物型燃料电池的膜电极组件（10）与醇水溶液接触，然后用水洗涤组件（10）。 然后，将膜电极组件（10）夹在双极板（30,31）之间以构成晶胞。 单元电池被夹在集电板（50,51）之间，堆叠夹在集电板之间的多个单位电池，堆叠被紧固并保持在绝缘板（60,61）和端板（70,71）之间， 以生产固体聚合物型燃料电池。

公开(公告)号：US06784289B2

公开(公告)日：2004-08-31

申请号：US09747007

申请日：2000-12-21

申请人： Thérèse Ouellet ,  Brian M. Miki ,  Elizabeth Foster ,  Teresa Martin-Heller ,  Lining Tian ,  Daniel C. Brown ,  Peijun Zhang ,  Jiro Hattori ,  Kamal Malik ,  Keqiang Wu ,  David A. Theilmann ,  Raymond Tropiano

发明人： Thérèse Ouellet ,  Brian M. Miki ,  Elizabeth Foster ,  Teresa Martin-Heller ,  Lining Tian ,  Daniel C. Brown ,  Peijun Zhang ,  Jiro Hattori ,  Kamal Malik ,  Keqiang Wu ,  David A. Theilmann ,  Raymond Tropiano

IPC分类号： C07H2104

CPC分类号： C12N15/67 ,  C12N15/8216

摘要： The present invention is directed to translational regulatory elements that mediate the amount of protein produced within a host capable of expressing a construct comprising one or more translational regulatory elements in operative association with a gene of interest. These translational regulatory elements were derived from T1275 (tCUP) and exhibit a high degree of similarity with members of the RENT family of repetitive elements. Translational regulatory elements are disclosed that either increase or decrease he amount of protein produced within the host organism. These translational elements are operative in a wide range of hosts including plant, animals, yeast, fungi and bacteria. Analogs, derivatives and fragments of these translational elements are also disclosed.

摘要翻译： 本发明涉及介导在能够表达包含与感兴趣的基因有效结合的一种或多种翻译调控元件的构建体的宿主内产生的蛋白质的量的翻译调控元件。 这些翻译调控元件来自T1275（tCUP），并且与RENT家族的重复元件的成员呈现高程度的相似性。 公开了翻译调控元件，即增加或减少宿主生物体内产生的蛋白质的量。 这些翻译元件在广泛的宿主中可操作，包括植物，动物，酵母，真菌和细菌。 还公开了这些翻译元件的类似物，衍生物和片段。

公开(公告)号：US5614232A

公开(公告)日：1997-03-25

申请号：US608221

申请日：1996-02-28

申请人： Shinji Torigoe ,  Jiro Hattori ,  Akimitsu Takagi

发明人： Shinji Torigoe ,  Jiro Hattori ,  Akimitsu Takagi

IPC分类号： A44B11/00 ,  A44B18/00 ,  B29C33/52 ,  B29C45/44 ,  B29D5/00 ,  B29D99/00 ,  F16B5/07 ,  B29C45/26

CPC分类号： B29C33/52 ,  B29C45/4457 ,  Y10S425/012

摘要： The present invention relates to an apparatus for forming a fastener member having a base and a plurality of headed stems arranged in columns and rows and projecting from the base. The fastener member is formed by injection molding a molten material into a base mold, sacrificial stem mold and head mold, wherein the material is solidified. After the base mold and head mold are removed, the stem mold may be removed to release the fastener member.

摘要翻译： 本发明涉及一种用于形成紧固件的装置，该装置具有底座和多个以杆和列排列并从基座突出的头部杆。 紧固件通过将熔融材料注塑成型模具，牺牲型茎模具和头模具而形成，其中材料被固化。 在移除基础模具和头部模具之后，可以移除杆模具以释放紧固件。

公开(公告)号：US5579562A

公开(公告)日：1996-12-03

申请号：US285175

申请日：1994-08-03

申请人： Jiro Hattori ,  Shinji Torigoe ,  Norihito Shibahara ,  Osamu Sawajiri ,  Hideo Matsumoto

发明人： Jiro Hattori ,  Shinji Torigoe ,  Norihito Shibahara ,  Osamu Sawajiri ,  Hideo Matsumoto

IPC分类号： A44B18/00 ,  F16B5/07

CPC分类号： F16B5/07 ,  Y10T24/27 ,  Y10T24/2708 ,  Y10T24/2775 ,  Y10T24/2792

摘要： An interengaging fastener is provided including two fastener members and structure for locating one fastener member with respect to the other fastener member. In one embodiment, the locating structure includes a protrusion extending from one fastener member and an opening formed in the other fastener member.

摘要翻译： 提供了一种相互接合的紧固件，其包括两个紧固件构件和用于相对于另一个紧固件构件定位一个紧固件构件的结构。 在一个实施例中，定位结构包括从一个紧固件构件延伸的突起和形成在另一个紧固件中的开口。