摘要:
In the conventional initial operation and activation processing (pre-processing), a processing time of ten odd hours or more is usually required, and special processing equipment and complex processing steps are needed. An aqueous alcohol solution is prepared, a membrane electrode assembly (10) for a solid polymer-type fuel cell is brought into contact with the aqueous alcohol solution, and the assembly (10) is then washed with water. Then, the membrane electrode assembly (10) is sandwiched between bipolar plates (30, 31) to configure a unit cell. The unit cell is sandwiched between collector plates (50, 51), a plurality of unit cells sandwiched between the collector plates are stacked, and the stack is tightened and held between insulating plates (60, 61) and end plates (70, 71) to produce a solid polymer-type fuel cell.
摘要:
T-DNA tagging with a promoterless .beta.-glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity constitutively. The gene fusion has been cloned and sequenced. It has been re-inserted into N. tabacum by Agrobacterium-mediated transformation. The N. tabacum DNA upstream from the GUS gene was approximately 2 kb in length and showed no homology to known sequences. This DNA, which contains a constitutive promoter, is useful in controlling the expression of exogenous genes in transgenic plants of diverse plant species.
摘要:
T-DNA tagging with a promoterless β-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.
摘要:
The present invention is directed to translational regulatory elements that mediate the amount of protein produced within a host capable of expressing a construct comprising one or more translational regulatory elements in operative association with a gene of interest. These translational regulatory elements were derived from T1275 (tCUP) and exhibit a high degree of similarity with members of the RENT family of repetitive elements. Translational regulatory elements are disclosed that either increase or decrease he amount of protein produced within the host organism. These translational elements are operative in a wide range of hosts including plant, animals, yeast, fungi and bacteria. Analogs, derivatives and fragments of these translational elements are also disclosed.
摘要:
A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.
摘要:
A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.
摘要:
The present invention relates to an apparatus for forming a fastener member having a base and a plurality of headed stems arranged in columns and rows and projecting from the base. The fastener member is formed by injection molding a molten material into a base mold, sacrificial stem mold and head mold, wherein the material is solidified. After the base mold and head mold are removed, the stem mold may be removed to release the fastener member.
摘要:
An interengaging fastener is provided including two fastener members and structure for locating one fastener member with respect to the other fastener member. In one embodiment, the locating structure includes a protrusion extending from one fastener member and an opening formed in the other fastener member.
摘要:
An acoustic decorative sheet includes a film layer, a glass cloth layer, and an adhesive layer separating the film layer from the glass cloth layer. The adhesive layer is adhered to the film layer and the glass cloth layer. The film layer and the adhesive layer have a plurality of micro bores disposed there through. The micro bores have a diameter in a range from 20 to 500 micrometers and number from 2 to 700 microbores per cm2. An acoustic assembly includes the acoustic decorative sheet, a substrate; and an air gap at least partially separating the acoustic decorative sheet from the substrate.
摘要:
A multilayer sheeting with a 3D floating image. The sheeting includes a layer of microlenses and a multilayer material disposed adjacent the microlenses. The multilayer material includes multiple adjacent layers having X-Y planar positions and a Z-direction orthogonal to the X-Y planar positions. Individual images, which contrast with the material, are formed in the multilayer material and include connected elements at interfaces between the multiple layers and conjunction elements between connected elements. The connected elements are registered in the Z-direction at the X-Y planar positions in the interfaces between the layers. The individual images collectively form a composite 3D image that appears to the unaided eye to be three-dimensional and floating above or below the sheeting, or both.