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    RNA/DNA nanoparticle for siRNA target-specific delivery and vehicle including the same
    3.
    发明申请
    RNA/DNA nanoparticle for siRNA target-specific delivery and vehicle including the same 审中-公开
    用于siRNA靶特异性递送的RNA / DNA纳米颗粒和包含其的载体

    公开(公告)号：US20160208245A1

    公开(公告)日：2016-07-21

    申请号：US14754556

    申请日：2015-06-29

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Hyung Jun AHN ,  Ick Chan KWON ,  Mihue JANG ,  Jong Hwan KIM

    IPC: C12N15/113

    CPC classification number: C12N15/111 ,  C12N2310/14 ,  C12N2310/3515 ,  C12N2310/531 ,  C12N2320/32

    Abstract: A RNA/DNA nanoparticle for delivering siRNA where a RNA transcript including at least one hairpin structure hybridizes DNA-cholesterol conjugate and folate-DNA conjugate including a complementary sequence to the RNA transcript, and a composition including the RNA/DNA nanoparticle is provided. More specifically, because various siRNA used for different applications can be contained in the RNA/DNA nanoparticle for delivering siRNA at a high loading efficiency, and has stability to the outer attacks such as nuclease degradation. The RNA/DNA nanoparticle siRNA can be prepared by self-assembly without using polycationic agent which is harmful agent for body. The folate targeting to various cancer cells can accumulate the nanoparticle selectively on target cancer cell after intravenous injection, and make excellent gene-silencing effect inside the cancer tissue, thereby being used as a good agent for treating cancers.

    Abstract translation: 提供了用于递送siRNA的RNA / DNA纳米颗粒，其中包含至少一个发夹结构的RNA转录物与DNA-胆固醇缀合物和包含与RNA转录物的互补序列的叶酸-DNA缀合物杂交，并且包含RNA / DNA纳米颗粒的组合物。 更具体地，因为用于不同应用的各种siRNA可以包含在用于以高负载效率递送siRNA的RNA / DNA纳米颗粒中，并且对外部攻击如核酸酶降解具有稳定性。 RNA / DNA纳米颗粒siRNA可以通过自组装制备，而不使用作为身体有害物质的聚阳离子。 针对各种癌细胞的叶酸可以在静脉注射后在靶癌细胞上选择性地积聚纳米颗粒，并且在癌组织内部具有优异的基因沉默效果，从而被用作治疗癌症的良好剂。

    METHOD FOR IN VIVO TARGETING OF NANOPARTICLES VIA BIOORTHOGONAL COPPER-FREE CLICK CHEMISTRY
    4.
    发明申请
    METHOD FOR IN VIVO TARGETING OF NANOPARTICLES VIA BIOORTHOGONAL COPPER-FREE CLICK CHEMISTRY 有权
    通过生物元素无铜点击化学方法对纳米颗粒进行生物分析

    公开(公告)号：US20130251784A1

    公开(公告)日：2013-09-26

    申请号：US13774247

    申请日：2013-02-22

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Kwang Meyung KIM ,  Ick Chan KWON ,  Kuiwon CHOI ,  Heebeom KOO ,  Sang-min LEE ,  Inchan YOUN

    IPC: A61K47/26 ,  A61K47/34

    CPC classification number: A61K47/26 ,  A61K47/34 ,  A61K47/549 ,  A61K47/555 ,  A61K47/6911 ,  A61K49/0032 ,  A61K49/0052

    Abstract: The present disclosure relates to a method for in vivo targeting of a nanoparticle via bioorthogonal copper-free click chemistry, more particularly to a method for in vivo targeting of a nanoparticle, including: injecting a precursor capable of being metabolically engineered in vivo when injected into a living system and having a first bioorthogonal functional group into the living system; and injecting a nanoparticle having a second bioorthogonal functional group which can perform a bioorthogonal copper-free click reaction with the first bioorthogonal functional group attached thereto into the living system.In accordance with the present disclosure, accumulation of nanoparticles at a target site in a living system can be increased remarkably and the biodistribution of the nanoparticles can be controlled since the nanoparticles bound to a cell surface are taken up into the cell with time.

    Abstract translation: 本公开内容涉及一种通过生物正交无铜点击化学体内靶向纳米颗粒的方法，更具体地涉及纳米颗粒的体内靶向方法，包括：注射能够在注射入体内时被代谢工程化的前体 生活系统，并具有第一生物正交功能组进入生活系统; 并且将具有第二生物正交官能团的纳米颗粒注射到与生物体系附着的第一生物正交官能团进行生物正交无铜点击反应。 根据本公开，可以显着增加生物体系中靶位点上的纳米颗粒的积累，并且可以控制纳米颗粒的生物分布，因为结合到细胞表面的纳米颗粒随时间被吸收到细胞中。

    METHOD FOR INDUCING TRANS-DIFFERENTIATION OF CARDIOMYOCYTES BASED ON EXOSOME
    6.
    发明申请
    METHOD FOR INDUCING TRANS-DIFFERENTIATION OF CARDIOMYOCYTES BASED ON EXOSOME 审中-公开

    公开(公告)号：US20200002677A1

    公开(公告)日：2020-01-02

    申请号：US16456127

    申请日：2019-06-28

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Ick Chan KWON ,  Sun Hwa KIM ,  Yoosoo YANG ,  Hyosuk KIM

    IPC: C12N5/077

    Abstract: The present invention relates to a method for inducing trans-differentiation of cardiomyocytes based on exosome, and more particularly, to a method for inducing trans-differentiation of a fibroblast into a cardiomyocyte, comprising the steps of: isolating exosomes in a culture medium during a process of differentiating a stem cell into the cardiomyocyte; culturing a fibroblast in a cardiomyocyte reprogramming medium containing the isolated exosomes; and culturing the fibroblast cultured in a cardiomyocyte differentiation medium containing the isolated exosomes.

    GELATIN-BASED NANOPARTICLE COMPLEX FOR TUMOR-TARGETED DELIVERY OF siRNA AND METHOD FOR PREPARING THE SAME
    8.
    发明申请
    GELATIN-BASED NANOPARTICLE COMPLEX FOR TUMOR-TARGETED DELIVERY OF siRNA AND METHOD FOR PREPARING THE SAME 有权
    用于肿瘤定向递送siRNA的基于胶原的纳米复合物及其制备方法

    公开(公告)号：US20150038690A1

    公开(公告)日：2015-02-05

    申请号：US14340845

    申请日：2014-07-25

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Kwang Meyung KIM ,  Sun Hwa KIM ,  Ick Chan KWON ,  Ji Young YHEE ,  Sojin LEE

    IPC: A61K47/48 ,  A61K31/7088

    CPC classification number: A61K31/7088 ,  A61K31/713 ,  A61K47/6435 ,  A61K47/6903 ,  A61K47/6929

    Abstract: Disclosed is a gelatin-based nanoparticle complex for tumor-targeted delivery of siRNA for specific gene silencing in tumor cells. The gelatin-based nanoparticle complex includes: poly-siRNA chains whose ends are modified with thiol groups; and thiolated gelatin bound to the poly-siRNA chains through disulfide crosslinking and charge interactions. The gelatin-based nanoparticle complex is not degraded in the bloodstream and can be efficiently absorbed into tumor cells without cytotoxicity. The delivered siRNA can effectively silence target gene expression. Also disclosed is a method for preparing the gelatin-based nanoparticle complex.

    Abstract translation: 公开了一种用于肿瘤靶向递送siRNA用于肿瘤细胞中特异性基因沉默的明胶基纳米颗粒复合物。 基于明胶的纳米颗粒复合物包括：末端用巯基修饰的多siRNA链; 并通过二硫键交联和电荷相互作用将与siRNA结合的多聚siRNA链结合在一起。 基于明胶的纳米颗粒复合物在血液中不降解，并且可以有效地吸收到肿瘤细胞中而没有细胞毒性。 递送的siRNA可以有效地沉默靶基因表达。 还公开了一种制备明胶基纳米颗粒复合物的方法。

    METHYLENE BLUE NANOPARTICLE FOR BIOIMAGING AND PHOTODYNAMIC THERAPY AND USE THEREOF
    10.
    发明申请
    METHYLENE BLUE NANOPARTICLE FOR BIOIMAGING AND PHOTODYNAMIC THERAPY AND USE THEREOF 审中-公开
    用于生物转化和光化学治疗的亚甲基蓝色纳米颗粒及其用途

    公开(公告)号：US20150273084A1

    公开(公告)日：2015-10-01

    申请号：US14667935

    申请日：2015-03-25

    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

    Inventor： Sehoon KIM ,  Ick Chan KWON ,  Sangyoup LEE

    IPC: A61K49/00 ,  A61K9/00 ,  A61K9/107 ,  A61K41/00

    CPC classification number: A61K41/0057 ,  A61K9/1075 ,  A61K9/5123 ,  A61K9/5146 ,  A61K49/003 ,  A61K49/0082

    Abstract: The present invention relates to a methylene blue nanoparticle for bioimaging and photodynamic therapy, and a use thereof as a cancer therapeutic agent and a contrast agent. The methylene blue nanoparticle of the present invention for use as a topical cancer targeting photo therapeutic agent is composed of only a material of which the composition is clinically used or derived from human bodies, and thus a nanopreparation in which a barrier to clinical entry is low and the possibility of commercialization is very high, exhibits near-infrared fluorescence along with cancer targeting property, capacity of generating singlet oxygen and the like, and thus may be used for both bioimaging diagnosis such as optical imaging, and cancer targeting photodynamic therapy.

    Abstract translation: 本发明涉及用于生物成像和光动力学治疗的亚甲基蓝纳米颗粒及其作为癌症治疗剂和造影剂的用途。 用作局部癌症靶向光疗法的本发明的亚甲基蓝纳米颗粒仅由组合物临床使用或衍生自人体的材料组成，因此其中临床进入的障碍低的纳米制备 并且商品化的可能性非常高，显示近红外荧光以及癌靶向性，产生单线态氧的能力等，因此可用于生物成像诊断例如光学成像和癌症靶向光动力疗法。

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