公开(公告)号：US20190300936A1

公开(公告)日：2019-10-03

申请号：US16382294

申请日：2019-04-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jacob K. FREUDENTHAL ,  Geoffrey DAHLHOFF ,  Kevin MAHER ,  Ming Song CHEN ,  Gary LIM ,  Ronald DANEHY ,  David FORTESCUE ,  Ming SHEN ,  Woon Liang Soh ,  Francis T. CHENG ,  Ruoyun WU ,  Thiam Chye LEE ,  Gary A. CHAPPELL ,  Chee Kiong LIM ,  Jeff DERE ,  Ted E. STRAUB ,  Jorge FONSECA ,  Kuan Moon BOO ,  Soo Yong LAU

IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00

Abstract: A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lends can be a bi-convex lens.

公开(公告)号：US20200232943A1

公开(公告)日：2020-07-23

申请号：US16487795

申请日：2018-02-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gary LIM ,  David LIU ,  Peng LI ,  Bin CAO ,  Kirk HIRANO ,  Alexander DUKHOVNY ,  John DIXON

IPC: G01N27/447

Abstract: A biological analysis device (100) for performing capillary electrophoresis includes a voltage section (124) configured to generate a voltage differential across a cathode (106) connector and an anode (116) connector, an optical detector system (112) configured to detect light emission from a sample, a temperature regulation section (126), and a cartridge holding portion configured to receive at least a portion of a removable cartridge (102) comprising one or more capillaries (104) and a separation medium container (118). The biological analysis device may include one or more actuators (122) configured to actuate components of the removable cartridge when the removable cartridge is received in the cartridge holding portion. Devices and methods relate to biological analysis.

公开(公告)号：US20160168626A1

公开(公告)日：2016-06-16

申请号：US15048324

申请日：2016-02-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald DANEHY ,  Gary LIM ,  Jacob FREUDENTHAL

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/50851 ,  B01L3/50855 ,  B01L3/50857 ,  B01L2200/026 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0893 ,  B01L2300/161 ,  C12M23/12 ,  C12M23/38

Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.

Abstract translation: 用于容纳多个生物样品的壳体包含基底，基底和盖。 底物附着到基底上，并且包括被配置成接收一个或多个生物样品的多个反应区域。 盖子包括内表面，并且被构造成附接到基座，使得基座和盖子一起提供容纳基底的空腔。 内表面包括中心区域和围绕中心区域设置的凹陷区域。 中心区域构造成覆盖反应区域，而凹陷区域在垂直于内表面的方向上偏离中心区域。

公开(公告)号：US20220283118A1

公开(公告)日：2022-09-08

申请号：US17703859

申请日：2022-03-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gary LIM ,  David LIU ,  Peng LI ,  Bin CAO ,  Kirk HIRANO ,  Alexander DUKHOVNY ,  John DIXON

IPC: G01N27/447

Abstract: A biological analysis device for performing capillary electrophoresis includes a voltage section configured to generate a voltage differential across a cathode connector and an anode connector, an optical detector system configured to detect light emission from a sample, a temperature regulation section, and a cartridge holding portion configured to receive at least a portion of a removable cartridge comprising one or more capillaries and a separation medium container. The biological analysis device may include one or more actuators configured to actuate components of the removable cartridge when the removable cartridge is received in the cartridge holding portion. Devices and methods relate to biological analysis.

公开(公告)号：US20130210674A1

公开(公告)日：2013-08-15

申请号：US13743217

申请日：2013-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ian A. HARDING ,  Donald SANDELL ,  Kevin BODNER ,  Jaynish PATEL ,  Timothy WOUDENBERG ,  Mark OLDHAM ,  H. Pin KAO ,  Gary LIM ,  John P. BODEAU ,  Dar BAHATT ,  Sergey V. ERMAKOV ,  Adrian FAWCETT ,  Jones C. Jones

IPC: C12N15/10

CPC classification number: C12N15/1072 ,  B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2300/021 ,  B01L2300/041 ,  B01L2300/0829 ,  B01L2300/12 ,  B01L2300/1805

Abstract: A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.