公开(公告)号：US20140324376A1

公开(公告)日：2014-10-30

申请号：US14330756

申请日：2014-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd REARICK ,  Melville DAVEY ,  Mark BEAUCHEMIN

IPC: G01N27/416

CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor may can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

Abstract translation: 提供了一种方法，计算机程序产品和系统来校准具有多个传感器的传感器阵列。 该方法可以包括将参考电极的电压从第一电压扫描到第二电压，其中参考电极与传感器阵列流体连通。 可以在第一和第二电压内的一个或多个电压监视多个传感器中的每一个的输出电压。 可以在一个或多个电压中的每一个处计算多个传感器的总体平均增益。 此外，可以确定传感器的采集窗口。 采集窗口可以包括在特定参考电极电压下提供最大总平均增益的传感器的最大分布。

公开(公告)号：US20170194985A1

公开(公告)日：2017-07-06

申请号：US15368765

申请日：2016-12-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Charles Sugnet ,  Simon CAWLEY ,  Mohit GUPTA ,  Iztok MARJANOVIC ,  Todd REARICK ,  Mark BEAUCHEMIN ,  Earl HUBBELL

IPC: H03M7/30 ,  G06F19/24

CPC classification number: H03M7/30 ,  G16B40/00

Abstract: A compression method includes measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises at least one region associated with expected measured values and at least one region associated with unpredictable measured values; applying a first compression process to the waveform, the first compression process including an averaging of one or more frames in one or more portions of the waveform; applying a keyframe delta compression to the frame-averaged data; forming a compressed data structure including a keyframe and calculated differences subsequent to the keyframe; and storing one or more compressed data structures in memory.

公开(公告)号：US20210116414A1

公开(公告)日：2021-04-22

申请号：US17063621

申请日：2020-10-05

Applicant: Life Technologies Corporation

Inventor： Todd REARICK ,  Melville DAVEY ,  Mark BEAUCHEMIN

IPC: G01N27/416 ,  G16B30/00 ,  G01N27/414

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

公开(公告)号：US20150355265A1

公开(公告)日：2015-12-10

申请号：US14830391

申请日：2015-08-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark BEAUCHEMIN

IPC: G01R31/26 ,  G01N27/414 ,  G01R31/28

CPC classification number: G01R31/2851 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/416 ,  G01R31/2621

Abstract: An apparatus including an array of sensors including a plurality of chemical sensors and a plurality of reference sensors, each chemical sensor coupled to a corresponding reaction region for receiving at least one reactant, and each reference sensor comprising a field effect transistor having a gate coupled to a corresponding reference line and an access circuit for accessing the chemical sensors and the reference sensors and a controller to apply bias voltages to the reference lines to select corresponding reference sensors, acquire output signals from the selected reference sensors, and identify one or more defects in the access circuit based on differences between the acquired output signals and expected output signals.

Abstract translation: 一种包括传感器阵列的设备，包括多个化学传感器和多个参考传感器，每个化学传感器耦合到对应的反应区域以接收至少一个反应物，每个参考传感器包括场效应晶体管，栅极耦合到 相应的参考线和用于访问化学传感器和参考传感器的访问电路以及用于向参考线施加偏置电压以选择相应的参考传感器的控制器，从所选择的参考传感器获取输出信号，以及识别一个或多个缺陷 基于所获取的输出信号和预期输出信号之间的差异的存取电路。

公开(公告)号：US20220154176A1

公开(公告)日：2022-05-19

申请号：US17525737

申请日：2021-11-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew WONG ,  Kylan SZETO ,  Shanti SHANKAR ,  Mark BEAUCHEMIN

IPC: C12N15/10 ,  C12Q1/6874 ,  C12Q1/6825 ,  G01N35/10

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

公开(公告)号：US20200075130A1

公开(公告)日：2020-03-05

申请号：US16543475

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shanti SHANKAR ,  Sean ZIMMERMAN ,  Mark BEAUCHEMIN ,  John THOMPSON

IPC: G16B45/00 ,  G06T1/00 ,  G16B30/10 ,  G01N1/28

Abstract: A method for user guided initiating of an instrument includes receiving a run plan via a user interface of the instrument; indicating on the user interface, based on the run plan, a consumable to be provided to the instrument; detecting the presence of the consumable using a vision system; and indicating the presence of the consumable via the user interface.

公开(公告)号：US20190041411A1

公开(公告)日：2019-02-07

申请号：US16100155

申请日：2018-08-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MARRAN ,  Mark BEAUCHEMIN

IPC: G01N33/84 ,  C12Q1/6874

Abstract: A method of preparing a sequencing device includes determining a sensitivity of a pH of a solution to a first reagent, determining an amount of the first reagent to add to the solution to approach a target pH, adding the amount of the first reagent to the solution, and diluting a nucleotide solution with the solution.

公开(公告)号：US20180313786A1

公开(公告)日：2018-11-01

申请号：US15920387

申请日：2018-03-13

Applicant: Life Technologies Corporation

Inventor： Todd REARICK ,  Melville DAVEY ,  Mark BEAUCHEMIN

IPC: G01N27/416 ,  G01N27/414 ,  G06F19/22

CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G16B30/00

Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

公开(公告)号：US20150355269A1

公开(公告)日：2015-12-10

申请号：US14830559

申请日：2015-08-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark BEAUCHEMIN

IPC: G01R31/28 ,  G01N27/414 ,  G01R31/26

Abstract: In one implementation, a method for operating an apparatus is described. The method includes applying a bias voltage to place a transistor of a reference sensor in a known state, the reference sensor in an array of sensors that further includes a chemical sensor coupled to a reaction region for receiving at least one reactant. The method further includes acquiring an output signal from the reference sensor in response to the applied bias voltage, and determining a defect associated with the array if the output signal does not correspond to the known state.

Abstract translation: 在一个实现中，描述了一种用于操作设备的方法。 所述方法包括施加偏置电压以将参考传感器的晶体管置于已知状态，所述参考传感器处于传感器阵列中，所述传感器阵列还包括耦合到用于接收至少一种反应物的反应区域的化学传感器。 该方法还包括响应于施加的偏置电压从参考传感器获取输出信号，以及如果输出信号不对应于已知状态，则确定与阵列相关联的缺陷。

公开(公告)号：US20130231870A1

公开(公告)日：2013-09-05

申请号：US13754566

申请日：2013-01-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Charles SUGNET ,  Simon CAWLEY ,  Mohit GUPTA ,  Iztok MARJANOVIC ,  Todd REARICK ,  Mark BEAUCHEMIN ,  Earl HUBBELL

IPC: H03M7/30

CPC classification number: H03M7/30 ,  G06F19/24

Abstract: A compression method includes measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises at least one region associated with expected measured values and at least one region associated with unpredictable measured values; applying a first compression process to the waveform, the first compression process including an averaging of one or more frames in one or more portions of the waveform; and applying a second compression process to the waveform, the second compression process including a truncating of data corresponding to a portion of the waveform that is not related to a nucleotide incorporation component of the waveform.

Abstract translation: 压缩方法包括测量与传感器阵列上发生的化学事件相关联的波形，其中所述波形包括与预期测量值相关联的至少一个区域和与不可预测的测量值相关联的至少一个区域; 对所述波形应用第一压缩处理，所述第一压缩处理包括所述波形的一个或多个部分中的一个或多个帧的平均值; 以及对所述波形应用第二压缩处理，所述第二压缩处理包括对应于与所述波形的核苷酸掺入成分无关的所述波形的一部分的数据的截断。