公开(公告)号：US20180237595A1

公开(公告)日：2018-08-23

申请号：US15871863

申请日：2018-01-15

Applicant: Life Technologies AS ,  Life Technologies Corporation

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  M. Talha GOKMEN ,  Pontus LUNDBERG ,  Prasanna Krishnan THWAR ,  Alfred LUI ,  Lily LU ,  Wolfgang HINZ ,  Lene Husaboe ,  Elisabeth BREIVOLD ,  Astrid Evenroed MOLTEBERG ,  Synne LARSEN

IPC: C08J3/075 ,  C08F8/12

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US20180223359A1

公开(公告)日：2018-08-09

申请号：US15945348

申请日：2018-04-04

Applicant: Life Technologies Corporation

Inventor： Steven M. MENCHEN ,  Alan BLANCHARD ,  Luisa ANDRUZZI ,  Shaheer H. KHAN ,  Dmitriy GREMYACHINSKIY ,  Alfred LUI ,  Craig STOLARCZYK ,  Tanya SOKOLSKY ,  Prasanna Krishnan THWAR

IPC: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US20170002152A1

公开(公告)日：2017-01-05

申请号：US15200533

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  Talha M. GOKMEN ,  Pontus LUNDBERG ,  Prasanna Krishnan THWAR ,  Alfred LUI ,  Lily LU ,  Wolfgang HINZ ,  Lene HUSABOE ,  Elisabeth BREIVOLD ,  Astrid Evenroed MOLTEBERG ,  Synne LARSEN

IPC: C08J3/075 ,  C08F220/70

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

Abstract translation: 聚合物基材如聚合物颗粒由羧基官能单体形成。 在一个实例中，羧基官能单体具有保护基代替羧基的OH。 一旦单体聚合，就可以除去这种保护基团，从而提供具有羧基官能位点的聚合物网络。 这种位点可用于将其它功能性附加到聚合物基材上。

公开(公告)号：US20160001249A1

公开(公告)日：2016-01-07

申请号：US14789650

申请日：2015-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Ronald L. CICERO ,  Yufang WANG ,  Alexander MASTROIANNI ,  Prasanna Krishnan THWAR ,  Jeremy GRAY ,  Marc GLAZER ,  Tommie Lloyd LINCECUM, JR. ,  Mohammad ALANJARY ,  Joseph KOSCINSKI

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00677 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2523/32 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/155 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. The beads at least partially deposit into the plurality of wells. The method also includes removing liquid from the flow cell, evaporating liquid from the flow cell, for example, by drawing air through the flow cell; and applying a hydrating solution to the flow cell.

Abstract translation: 在传感器基板上加载珠粒的方法包括将包含珠粒的悬浮液施加到在传感器基底上限定的流动池中。 传感器基板包括多个孔。 珠粒至少部分沉积到多个孔中。 该方法还包括从流动池中去除液体，从流动池中蒸发液体，例如通过抽吸空气流过流动池; 以及将水合溶液施加到所述流动池。

公开(公告)号：US20230407391A1

公开(公告)日：2023-12-21

申请号：US18209766

申请日：2023-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. MENCHEN ,  Alan BLANCHARD ,  Luisa ANDRUZZI ,  Shaheer KHAN ,  Dmitriy GREMYACHINSKIY ,  Alfred LUI ,  Craig STOLARCZYK ,  Tanya SOKOLSKY ,  Prasanna Krishnan THWAR

IPC: C12Q1/6876 ,  C12Q1/6834 ,  C08F8/40

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C08F8/40

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US20190390018A1

公开(公告)日：2019-12-26

申请号：US16253141

申请日：2019-01-21

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Geir FONNUM ,  Steven M. MENCHEN ,  M. Talha GOKMEN ,  Pontus LUNDBERG ,  Prasanna Krishnan THWAR ,  Alfred LUI ,  Lily LU ,  Wolfgang HINZ ,  Lene HUSABOE ,  Elisabeth BREIVOLD ,  Astrid Evenroed MOLTEBERG ,  Synne LARSEN

IPC: C08J3/075 ,  C08F220/70 ,  C08F8/12 ,  C08F220/58

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US20170009275A1

公开(公告)日：2017-01-12

申请号：US15202487

申请日：2016-07-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. MENCHEN ,  Guobing XIANG ,  Prasanna Krishnan THWAR ,  Jeffrey FREDIANI ,  Allen WONG ,  Alfred LUI ,  Lily LU

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q2565/518 ,  C12Q2525/117 ,  C12Q2525/197 ,  C12Q2565/549 ,  C12Q2563/149

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

Abstract translation: 水凝胶网络包括具有偶联位点的水凝胶聚合物，在末端与偶联位点处的水凝胶聚合物共轭的寡核苷酸和耦合在寡核苷酸的末端与偶联位点之间的功能部分。 这样的水凝胶网络可以通过以下方法形成：包括活化底物的偶联位点并将与寡核苷酸的末端连接的连接体结合到活化的偶联位点，连接在寡核苷酸的末端和 接头部分。