公开(公告)号：US09243085B2

公开(公告)日：2016-01-26

申请号：US13762941

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Modahl ,  Nini Kjus ,  Astrid Evenroed Molteberg ,  Diem Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F2/16 ,  C08F230/08 ,  C08F8/34 ,  C08F6/24 ,  C07F7/18 ,  C08F130/08 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F222/38 ,  C08F8/32 ,  C12Q1/68

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09062079B2

公开(公告)日：2015-06-23

申请号：US13762489

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/18 ,  C07F7/10 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，将产物与第一溶液分离，并在第二反应条件下将产物与丙烯酰氯混合 形成甲硅烷基保护的二丙烯酰胺化合物的第二溶液。

公开(公告)号：US20200308630A1

公开(公告)日：2020-10-01

申请号：US16849833

申请日：2020-04-15

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Geir Fonnum ,  Nini Kjus

IPC: C12Q1/6806 ,  C12N15/10

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US10017586B2

公开(公告)日：2018-07-10

申请号：US15453750

申请日：2017-03-08

Applicant: LIFE TECHNOLOGIES AS

Inventor： Grete Modahl ,  Geir Fonnum ,  Astrid Molteberg ,  Silje Lien ,  Nini Kjus

IPC: B32B5/16 ,  B32B9/00 ,  C08F4/46 ,  C08F2/22 ,  C08J9/20

CPC classification number: C08F2/22 ,  C08F8/30 ,  C08F12/08 ,  C08F212/36 ,  C08F257/02 ,  C08F2800/20 ,  C08J3/128 ,  C08J9/18 ,  C08J9/20 ,  C08J2325/08 ,  C12N11/08 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/2998 ,  C08F212/08

Abstract: This invention relates to monodisperse cross-linked polymer particles, comprising particles with a substantially smooth outer surface and an average diameter of less than 1 μm, wherein the particles are solid or porous, and wherein the coefficient of variation (CV) % of the particles, when measured by CPS disk centrifugation analysis, is less than 15%. These monodisperse cross-linked polymer particles may comprise magnetic material and are useful in various application. This invention also relates to monodisperse polymer particles for use as seed particles in the Ugelstad process.

公开(公告)号：US11261480B2

公开(公告)日：2022-03-01

申请号：US16849833

申请日：2020-04-15

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Geir Fonnum ,  Nini Kjus

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US09909165B2

公开(公告)日：2018-03-06

申请号：US15431678

申请日：2017-02-13

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Geir Fonnum ,  Nini Kjus

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support while maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US20130210991A1

公开(公告)日：2013-08-15

申请号：US13762941

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Geir Fonnum ,  Grete Modahl ,  Nini Kjus ,  Astrid Evenroed Molteberg ,  Diem Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F6/24

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Abstract translation: 形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元，从而形成包含多个疏水保护基团的聚合物颗粒。 该方法还包括将聚合物颗粒转化为亲水性颗粒。

公开(公告)号：US20130211119A1

公开(公告)日：2013-08-15

申请号：US13762489

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nini Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/18

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.