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公开(公告)号：US20130260370A1
公开(公告)日：2013-10-03
申请号：US13876022
申请日：2011-11-30
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises a metal silicate, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供浓缩装置，其包括（1）多孔纤维非织造基质和（2）至少一种包含金属硅酸盐的浓缩剂的颗粒，所述颗粒 位于多孔纤维非织造基体中; （b）提供包含至少一种靶细胞分析物的样品; （c）使浓缩装置与样品接触，使得至少一部分目标细胞分析物与浓缩装置结合或捕获; 和（d）检测至少一种结合的靶细胞分析物的存在。
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公开(公告)号：US20130244225A1
公开(公告)日：2013-09-19
申请号：US13825676
申请日：2011-11-23
IPC分类号： G01N1/40 , G01N33/569
CPC分类号： G01N1/405 , B01D2239/0442 , B01D2239/0618 , C12M47/02 , C12Q1/06 , G01N33/56911 , G01N2001/4088 , Y02A50/52
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises diatomaceous earth, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供浓缩装置，其包括（1）多孔纤维非织造基质和（2）至少一种包含硅藻土的浓缩剂的颗粒，所述颗粒为 嵌入多孔纤维非织造基质中; （b）提供包含至少一种靶细胞分析物的样品; （c）使浓缩装置与样品接触，使得至少一部分目标细胞分析物与浓缩装置结合或捕获; 和（d）检测至少一种结合的靶细胞分析物的存在。
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公开(公告)号：US20120009569A1
公开(公告)日：2012-01-12
申请号：US13257406
申请日：2010-03-22
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising ferric oxide, titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供包含烧结多孔聚合物基质的浓缩装置，所述多孔聚合物基质包含至少一种含有硅藻土的浓缩剂，所述浓缩剂在其表面的至少一部分上具有包括 包含氧化铁，二氧化钛，细纳米金或铂的表面改性剂或其组合; （b）提供包含至少一种微生物菌株的样品; 和（c）使浓缩装置与样品接触，使得至少一部分微生物菌株与浓缩装置结合或捕获。
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公开(公告)号：US20120034621A1
公开(公告)日：2012-02-09
申请号：US13257382
申请日：2010-03-22
IPC分类号： C12Q1/04 , C12N7/02 , C12N1/14 , C08J9/00 , C12N1/20 , C12N1/10 , C08K3/34 , G01N33/569 , C12N1/16
CPC分类号： C08J9/0066 , B01D61/147 , B01D63/00 , C08J9/0061 , C08J2323/06 , C08J2423/06 , C12Q1/24
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供包含烧结的多孔聚合物基质的浓缩装置，所述多孔聚合物基质包含至少一种浓缩剂，所述浓缩剂包含无定形金属硅酸盐，并且具有具有金属原子与硅原子的表面组成 比例小于或等于0.5，如通过X射线光电子能谱（XPS）测定的; （b）提供包含至少一种微生物菌株的样品; 和（c）使浓缩装置与样品接触，使得至少一部分微生物菌株与浓缩装置结合或捕获。
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公开(公告)号：US09964474B2
公开(公告)日：2018-05-08
申请号：US13257406
申请日：2010-03-22
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising ferric oxide, titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.
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公开(公告)号：US08969011B2
公开(公告)日：2015-03-03
申请号：US13257382
申请日：2010-03-22
IPC分类号： G01N33/53 , C12Q1/04 , C12Q1/02 , C12N7/02 , C12N1/20 , C12N7/00 , C12N1/12 , C12N1/10 , B01D61/14 , B01D63/00 , C12Q1/24 , C12N7/04
CPC分类号： C08J9/0066 , B01D61/147 , B01D63/00 , C08J9/0061 , C08J2323/06 , C08J2423/06 , C12Q1/24
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供包含烧结的多孔聚合物基质的浓缩装置，所述多孔聚合物基质包含至少一种浓缩剂，所述浓缩剂包含无定形金属硅酸盐，并且具有具有金属原子与硅原子的表面组成 比例小于或等于0.5，如通过X射线光电子能谱（XPS）测定的; （b）提供包含至少一种微生物菌株的样品; 和（c）使浓缩装置与样品接触，使得至少一部分微生物菌株与浓缩装置结合或捕获。
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7.发明申请公开(公告)号：US20120276580A1
公开(公告)日：2012-11-01
申请号：US13511169
申请日：2010-12-17
CPC分类号： C12N1/02 , B01J20/041 , B01J20/06 , B01J20/10 , B01J20/14 , B01J20/3071 , B01J20/3204 , B01J20/3236 , G01N1/405
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing an adsorption buffer-modified inorganic concentration agent that is prepared by a process comprising (1) contacting at least one inorganic concentration agent with at least one cation-containing salt solution, so as to wet at least a portion of the inorganic concentration agent and (2) drying the resulting at least partially wet inorganic concentration agent; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the adsorption buffer-modified inorganic concentration agent with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the adsorption buffer-modified inorganic concentration agent.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供吸附缓冲剂改性的无机浓缩剂，其通过以下方法制备，所述方法包括：（1）使至少一种无机浓缩剂与至少一种含阳离子的盐溶液 以至少润湿至少一部分无机浓缩剂，和（2）干燥得到的至少部分湿的无机浓缩剂; （b）提供包含至少一种微生物菌株的样品; 和（c）使吸附缓冲液改性的无机浓缩剂与样品接触，使得至少一部分微生物菌株与吸附缓冲液改性的无机浓缩剂结合或捕获。
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公开(公告)号：US20110318814A1
公开(公告)日：2011-12-29
申请号：US13142292
申请日：2009-12-30
CPC分类号： G01N1/4077 , C12M47/04 , C12Q1/24 , G01N1/405 , G01N33/1866 , G01N2001/4088
摘要： A method for isolating microorganisms from a sample, the sample including sample matrix and microorganisms, the method including the steps of providing a receptacle, the receptacle configured to allow filtering of the sample and to reversibly contain the sample and a concentration agent; adding the sample to the receptacle, wherein a microorganism-bound composition will be formed in the receptacle, the microorganism-bound composition including concentration agent-bound microorganisms and sample matrix; and filtering the microorganism-bound composition through a filter to collect the concentration agent-bound microorganisms on the filter, wherein the filter has an average pore size that is greater than the average size of the microorganisms. Kits and systems are also disclosed herein.
摘要翻译： 一种用于从样品中分离微生物的方法，所述样品包括样品基质和微生物，所述方法包括提供容器的步骤，所述容器被配置为允许样品过滤并且可逆地容纳样品和浓缩剂; 将样品加入到容器中，其中将在容器中形成微生物结合的组合物，微生物结合的组合物包括与浓度剂结合的微生物和样品基质; 并通过过滤器过滤微生物结合的组合物以收集过滤器上与浓度剂结合的微生物，其中过滤器的平均孔径大于微生物的平均尺寸。 本文还公开了套件和系统。
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公开(公告)号：US09624464B2
公开(公告)日：2017-04-18
申请号：US12678362
申请日：2008-10-02
IPC分类号： G01N33/53 , C12Q1/04 , C12Q1/02 , C12N7/02 , C12N1/20 , C12N7/00 , C12N1/12 , C12N1/10 , C12N1/02 , C12N11/14 , C12Q1/24 , G01N33/543
CPC分类号： G01N33/54313 , C12N1/02 , C12N11/14 , C12Q1/04 , C12Q1/24 , G01N33/552 , G01N33/553
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to about 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.
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公开(公告)号：US09145541B2
公开(公告)日：2015-09-29
申请号：US12679063
申请日：2008-10-02
IPC分类号： C12N1/00 , C12Q1/24 , C12N11/00 , C12N1/02 , B01J20/02 , B01J20/06 , B01J20/28 , C12N11/14 , C12Q1/04 , G01N1/40 , G01N33/543
CPC分类号： C12N1/02 , B01J20/0229 , B01J20/06 , B01J20/28004 , B01J20/2803 , C12N11/14 , C12Q1/04 , C12Q1/24 , G01N1/405 , G01N33/54313
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a particulate concentration agent that comprises gamma-FeO(OH); (b) providing a fluid sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the concentration agent is dispersed in the sample and at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.
摘要翻译： 用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供包含γ-FeO（OH）的颗粒浓缩剂; （b）提供包含至少一种微生物菌株的流体样品; 和（c）使浓缩剂与样品接触，使得至少一部分浓缩剂分散在样品中，并且至少一部分微生物菌株与浓缩剂结合或捕获。
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