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    Esterification or hydrolysis with substrate treated un-dried immobilized lipolytic enzyme
    1.
    发明授权
    Esterification or hydrolysis with substrate treated un-dried immobilized lipolytic enzyme 失效
    用底物处理的未干燥的固定化脂肪分解酶进行酯化或水解

    公开(公告)号：US06716610B2

    公开(公告)日：2004-04-06

    申请号：US09453078

    申请日：1999-12-02

    申请人： Masami Shimizu ,  Toshiteru Komatsu ,  Masao Shimizu ,  Minoru Kase

    发明人： Masami Shimizu ,  Toshiteru Komatsu ,  Masao Shimizu ,  Minoru Kase

    IPC分类号： C12P764

    CPC分类号： C12P7/6418 ,  C12N11/08 ,  C12P7/6454

    摘要： A lipolytic enzyme such as lipase is adsorbed on a porous anion-exchange resin carrier to obtain an immobilized enzyme without drying. The un-dried immobilized enzyme is contacted with a fat or oil for a time which may be about 2 hours to about 24 hours to stabilize the immobilized enzyme, and the immobilized enzyme is recovered from the fat or oil. The recovered immobilized enzyme is then used for esterifying or hydrolyzing a substrate. Water content of the un-dried immobilized enzyme may be 20% or more be weight such as 20-60%. Prior to immobilization, the resin carrier may be treated with a lypophylic aliphatic acid which preferably has 8 to 18 carbons.

    摘要翻译： 将脂肪酶等脂肪分解酶吸附在多孔阴离子交换树脂载体上，得到不干燥的固定化酶。 将未干燥的固定化酶与脂肪或油接触约2小时至约24小时以稳定固定化酶的时间，并且从脂肪或油中回收固定化酶。 然后将回收的固定化酶用于酯化或水解底物。 未干燥固定化酶的含水量可以为20〜20重量％的20重量％以上。 在固定之前，树脂载体可以用优选具有8至18个碳原子的脂环族脂族酸处理。

    Method for manufacturing refined fat or oil
    4.
    发明授权
    Method for manufacturing refined fat or oil 有权
    制造精制脂肪或油的方法

    公开(公告)号：US08927039B2

    公开(公告)日：2015-01-06

    申请号：US13516415

    申请日：2010-12-14

    申请人： Minoru Kase ,  Tetsuya Abe ,  Shinpei Fukuhara ,  Toshiteru Komatsu ,  Keiji Shibata

    发明人： Minoru Kase ,  Tetsuya Abe ,  Shinpei Fukuhara ,  Toshiteru Komatsu ,  Keiji Shibata

    IPC分类号： C11B3/10 ,  C11B3/14 ,  A23D9/02 ,  A23D7/02

    CPC分类号： A23D7/02 ,  C11B3/10 ,  C11B3/14

    摘要： Provided is a method for manufacturing refined fats and oils. Here fats and oils are brought into contact with an adsorbent and subsequently treated with water vapor under at least one condition of the following conditions. In condition I, the time for which the fats and oils are brought into contact with the water vapor in a temperature range of 175° C. to 205° C. for from 5 to 110 minutes. In condition 2, the fats and oils are brought into contact with the water vapor in a temperature range of 205° C. to 215° C. for from 5 to 50 minutes. In condition 3, the fats and oils are brought into contact with the water vapor in a temperature range of 215° C. to 230° C. for from 5 to 30 minutes.

    摘要翻译： 提供了一种制造精炼脂肪和油的方法。 这里使脂肪和油与吸附剂接触，随后在至少一种以下条件的条件下用水蒸气处理。 在条件I中，使脂肪和油在175℃至205℃的温度范围内与水蒸气接触的时间为5至110分钟。 在条件2中，使油脂与水蒸汽在205〜215℃的温度范围内接触5〜50分钟。 在条件3中，使油脂与水蒸气在215〜230℃的温度范围内接触5〜30分钟。

    Process for producing fatty acids
    7.
    发明授权
    Process for producing fatty acids 有权
    生产脂肪酸的方法

    公开(公告)号：US08323934B2

    公开(公告)日：2012-12-04

    申请号：US12067245

    申请日：2006-10-06

    申请人： Minoru Kase ,  Toshiteru Komatsu

    发明人： Minoru Kase ,  Toshiteru Komatsu

    IPC分类号： C12N11/00 ,  C12N11/14 ,  C12N11/16 ,  C12P1/00 ,  C12P7/00 ,  C12P7/40 ,  C12P7/64

    CPC分类号： C11C1/045 ,  C12P7/6418

    摘要： A process for producing fatty acid by hydrolyzing oil and fat, which comprises: a first step of partially hydrolyzing oil and fat by either of the following method (a) or (b), and a second step of hydrolysis by the other method: (a) enzymatic hydrolysis using an immobilized enzyme which is an enzyme immobilized on a support and (b) high pressure and temperature hydrolysis. A method of efficiently producing fatty acid with a reduced content of trans-unsaturated fatty acid in the constituent fatty acids and having a good appearance with reduced coloring by hydrolysis of oil and fat is provided.

    摘要翻译： 一种通过水解油脂生产脂肪酸的方法，其包括：通过以下方法（a）或（b）之一部分水解油脂的第一步骤和通过其它方法水解的第二步骤：（ a）使用固定化酶（其是固定在载体上的酶）和（b）高压和高温水解的酶水解。 提供了一种在构成脂肪酸中有效地生产具有降低的反式不饱和脂肪酸含量的脂肪酸的方法，并且具有外观良好，油脂脂肪水解降低着色的方法。

    PROCESS FOR PRODUCING DIACYLGLYCEROL-RICH FAT OR OIL
    9.
    发明申请
    PROCESS FOR PRODUCING DIACYLGLYCEROL-RICH FAT OR OIL 审中-公开
    生产二油酸甘油或油的方法

    公开(公告)号：US20110076358A1

    公开(公告)日：2011-03-31

    申请号：US12995096

    申请日：2009-02-09

    申请人： Minoru Kase ,  Toshiteru Komatsu

    发明人： Minoru Kase ,  Toshiteru Komatsu

    IPC分类号： A23D9/02

    CPC分类号： C12P7/6454

    摘要： Provided is a process for producing a diacylglycerol-rich fat or oil efficiently under an industrially advantageous condition. Provided is a process for producing a diacylglycerol-rich fat or oil, including subjecting monoacylglycerols to a transesterification reaction by using a lipase in the presence of water.

    摘要翻译： 提供了在工业上有利的条件下有效生产含二酰基甘油的脂肪或油的方法。 提供了生产富含二酰基甘油的脂肪或油的方法，包括在水存在下使用脂肪酶使单酰基甘油进行酯交换反应。

    METHOD FOR PRODUCTION OF IMMOBILIZED ENZYME
    10.
    发明申请
    METHOD FOR PRODUCTION OF IMMOBILIZED ENZYME 审中-公开
    生产固定化酶的方法

    公开(公告)号：US20110053229A1

    公开(公告)日：2011-03-03

    申请号：US12988063

    申请日：2009-04-20

    申请人： Shinpei Fukuhara ,  Minoru Kase ,  Toshiteru Komatsu

    发明人： Shinpei Fukuhara ,  Minoru Kase ,  Toshiteru Komatsu

    IPC分类号： C12P7/64 ,  C12N11/00 ,  C12N11/08 ,  C12P7/62

    CPC分类号： C12N11/00 ,  C12N9/20 ,  C12N11/08 ,  C12P7/62 ,  C12P7/6454

    摘要： Provided is a method of producing an immobilized lipolytic enzyme which has almost the same ability as that of an unused immobilized enzyme by effectively utilizing an immobilization carrier in a used immobilized enzyme which has been used in an esterification reaction.The method of producing an immobilized lipolytic enzyme includes: mixing an immobilized lipolytic enzyme that has been used in an esterification reaction with a solvent to wash the immobilized lipolytic enzyme so that the residual oil content in the immobilized lipolytic enzyme is reduced to 50 parts by mass or less based on 100 parts by mass of an immobilization carrier; bringing the immobilized lipolytic enzyme into contact with an alkali solution; collecting the immobilization carrier; and adsorbing a lipolytic enzyme on the immobilization carrier.

    摘要翻译： 本发明提供一种通过在用于酯化反应的使用的固定化酶中有效利用固定化载体的方法，生产与未使用的固定化酶几乎相同的固定化的脂肪分解酶的方法。 制备固定化的脂肪分解酶的方法包括：将已经用于酯化反应的固定化的脂肪分解酶与溶剂混合以洗涤固定化的脂肪分解酶，使得固定化的脂肪分解酶中的残留油含量降低到50质量份 或更少，基于100质量份的固定化载体; 使固定化的脂肪分解酶与碱溶液接触; 收集固定载体; 并将脂肪分解酶吸附在固定载体上。