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    Monoclonal antibodies against melanoma
    2.
    发明授权
    Monoclonal antibodies against melanoma 失效
    针对黑色素瘤的单克隆抗体

    公开(公告)号：US5610280A

    公开(公告)日：1997-03-11

    申请号：US214020

    申请日：1994-03-15

    申请人： Michael Brandt ,  Josef Endl ,  Herbert Jungfer ,  Winfried Albert

    发明人： Michael Brandt ,  Josef Endl ,  Herbert Jungfer ,  Winfried Albert

    IPC分类号： A61K39/395 ,  A61P35/00 ,  C07K16/00 ,  C07K16/30 ,  C07K19/00 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/574 ,  G01N33/577 ,  A61K51/10 ,  C12N5/12 ,  G01N33/53

    CPC分类号： C07K16/3053

    摘要： The present invention provides a human monoclonal antibody against melanoma, characterized in that it binds to the gangliosides GM3 and GD3 but essentially does not bind to the gangliosides GM1, GM2, GD1a, GD1b and GD2, the binding of the antibody to the gangliosides having been determined by immune staining after thin layer chromatographic separation of the gangliosides. The present invention also provides a process for the production of human monoclonal antibodies directed against melanoma, wherein, without previous immunization, B-lymphocytes are isolated from a healthy person, the isolated B-lymphocytes are immortalized, antibodies from the immortalized B-lymphocytes are screened by immune-histochemical analysis for binding against melanoma and/or melanoma metastases, the positively reacting B-lymphocytes are selected, cultured and monoclonal antibodies obtained therefrom.

    摘要翻译： 本发明提供了针对黑素瘤的人单克隆抗体，其特征在于其结合神经节苷脂GM3和GD3，但基本上不结合神经节苷脂GM1，GM2，GD1a，GD1b和GD2，抗体与神经节苷脂的结合 通过神经节苷脂薄层色谱分离后的免疫染色测定。 本发明还提供了一种制备针对黑素瘤的人单克隆抗体的方法，其中，在没有先前免疫的情况下，从健康人分离B淋巴细胞，分离的B淋巴细胞被永生化，来自永生化B淋巴细胞的抗体是 通过用于结合黑素瘤和/或黑素瘤转移的免疫组织化学分析进行筛选，选择阳性反应性B淋巴细胞，培养和从其获得的单克隆抗体。

    Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof
    3.
    发明授权
    Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof 失效
    与谷氨酸脱羧酶特异性结合的同种型IgG的单克隆抗体及其用途

    公开(公告)号：US06214568B1

    公开(公告)日：2001-04-10

    申请号：US09124410

    申请日：1998-07-29

    申请人： Josef Endl ,  Michael Brandt ,  Herbert Jungfer ,  Winfried Albert ,  Rosemarie Kientsch-Engel ,  Werner Scherbaum ,  Wiltrud Richter ,  Thomas Eiermann

    发明人： Josef Endl ,  Michael Brandt ,  Herbert Jungfer ,  Winfried Albert ,  Rosemarie Kientsch-Engel ,  Werner Scherbaum ,  Wiltrud Richter ,  Thomas Eiermann

    IPC分类号： G01N33573

    CPC分类号： C07K16/40 ,  C07K14/47 ,  C07K16/18 ,  C07K16/4241 ,  C07K2317/21

    摘要： The invention concerns human monoclonal antibodies of the IgG isotype against human pancreatic islet cells which can be obtained by immortalizing human lymphocytes of prediabetics or diabetics, treating the culture supernatant of the immortalized cells with a conjugate of antibodies against human Fc &ggr; and a label, subsequently treating with human immunoglobulin, incubating with immobilized human pancreatic islet cells identifying an immortalized human cell culture which produces an antibody against pancreatic islet cells via determination of the label bound to the immobilized islet cells, isolating a human immortalized cell which produces this antibody, propagating this immortalized cell and isolating the monoclonal antibody produced by these cells. The invention also concerns a process for the isolation of an islet cell antigen to which such antibodies bind as well as a method for the determination of antibodies against an islet cell antigen of the pancreas.

    摘要翻译： 本发明涉及针对人胰岛细胞的IgG同种型的人单克隆抗体，其可以通过使老年糖尿病患者或糖尿病患者的人淋巴细胞永生化而获得，用抗Fcγ和标记的抗体缀合物处理永生化细胞的培养物上清液，随后 用人免疫球蛋白处理，与固定的人胰岛细胞孵育，鉴定永生化​​的人细胞培养物，其通过测定与固定化胰岛细胞结合的标记产生抗胰岛细胞的抗体，分离产生该抗体的人永生化细胞，传播该抗体 永生化细胞并分离由这些细胞产生的单克隆抗体。 本发明还涉及用于分离这种抗体结合的胰岛细胞抗原的方法以及用于测定抗胰岛细胞抗原的抗体的方法。

    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor
    4.
    发明授权
    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor 失效
    用于定量细胞群或亚群的方法和适合其的试剂

    公开(公告)号：US4876189A

    公开(公告)日：1989-10-24

    申请号：US927803

    申请日：1986-11-05

    申请人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    发明人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    IPC分类号： G01N33/53 ,  C12Q1/28 ,  C12Q1/34 ,  G01N33/536 ,  G01N33/543 ,  G01N33/569

    CPC分类号： G01N33/56972 ,  G01N33/56966 ,  Y10S435/81 ,  Y10S435/967 ,  Y10T436/101666

    摘要： A process for quantification of cell populations or subpopulations, by incubating a sample with labelled antibodies directed against characteristic surface antigens of the cell population to be quantified to form labelled antibody/antigen complexes. Standards with known, differing particle concentration and having comparable sedimentation behavior to the cells to be determined and further, carrying molecules which are directed against the labelled antibody or a part hereof are also incubated with the labelled antibodies. The cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies and the amount of the labelling is measured not only on the cells but also on the particles. By comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells to be determined in the sample. The standard and a reagent kit for carrying out the process are also the subject of this application.

    摘要翻译： 通过将样品与针对要定量的细胞群的特征性表面抗原的标记抗体孵育以形成标记的抗体/抗原复合物，来定量细胞群体或亚群体的方法。 具有已知的，不同的颗粒浓度并且具有与要测定的细胞具有可比较的沉降行为的标准品，并且进一步携带针对标记的抗体或其部分的分子也与标记的抗体一起温育。 将样品溶液的细胞以及标准溶液的颗粒与过量标记的抗体分离出来，并且不仅在细胞上而且在颗粒上测量标记量。 通过将来自样品的测量值与来自标准溶液的测量值的比较，确定样品中待确定的细胞数量。 用于执行该过程的标准品和试剂盒也是本申请的主题。

    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor
    9.
    发明授权
    Process for the quantification of cell populations or subpopulations and a reagent suitable therefor 失效
    细胞群体或子囊的定量方法及其适合的试剂

    公开(公告)号：US5094940A

    公开(公告)日：1992-03-10

    申请号：US376707

    申请日：1989-07-07

    申请人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    发明人： Hartmut Schetters ,  Josef Endl ,  Winfried Albert

    IPC分类号： G01N33/53 ,  C12Q1/28 ,  C12Q1/34 ,  G01N33/536 ,  G01N33/543 ,  G01N33/569

    CPC分类号： G01N33/56972 ,  G01N33/56966 ,  Y10S435/81 ,  Y10S435/967 ,  Y10T436/101666

    摘要： A process for quantification of cell populations or subpopulations, by incubating a sample with labelled antibodies directed against characteristic surface antigens of the cell population to be quantified to form labelled antibody/antigen complexes. Standards with known, differing particle concentration and having comparable sedimentation behaviour to the cells to be determined and further, carrying molecules which are directed against the labelled antibody or a part hereof are also incubated with the labelled antibodies. The cells of the sample solution, as well as the particles of the standard solution, are separated off from the excess labelled antibodies and the amount of the labelling is measured not only on the cells but also on the particles. By comparison of the measurement value from the sample with the measurement values from the standard solutions, there is ascertained the number of cells to be determined in the sample. The standard and a reagent kit for carrying out the process are also the subject of this application.