公开(公告)号：US20140208836A1

公开(公告)日：2014-07-31

申请号：US14238653

申请日：2012-08-22

申请人： James P. Murphy ,  Michael J. Tomany ,  Joseph D. Michienzi ,  Paul Rainville ,  Robert S. Plumb ,  Geoff C. Gerhardt ,  Moon Chul Jung

发明人： James P. Murphy ,  Michael J. Tomany ,  Joseph D. Michienzi ,  Paul Rainville ,  Robert S. Plumb ,  Geoff C. Gerhardt ,  Moon Chul Jung

IPC分类号： G01N30/06

CPC分类号： G01N30/06 ,  B01L3/502715 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1827 ,  G01N30/16 ,  G01N30/6095 ,  G01N30/88 ,  G01N33/49 ,  G01N2001/383 ,  G01N2030/009 ,  G01N2030/062

摘要： An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.

摘要翻译： 用于色谱系统的装置包括具有配置为分析色谱柱的流体通道和在微流体衬底的一侧上的流体端口的微流体衬底。 流体端口在分析色谱柱的头端打开。 干血斑（DBS）收集装置容纳一个或多个干燥的生物样品。 DBS收集装置直接耦合到微流体底物，其中一个生物样品被放置成与微流体底物的流体通道流体连通，并且该生物样品的提取流向分析色谱柱的头端。 流体耦合到流体端口的稀释剂源将溶剂提供到分析柱的头端，以在生物样品流入分析色谱柱之前稀释提取的生物样品。

公开(公告)号：US20130056631A1

公开(公告)日：2013-03-07

申请号：US13698336

申请日：2011-05-09

申请人： Michael J. Tomany ,  James P. Murphy ,  Paul Rainville

发明人： Michael J. Tomany ,  James P. Murphy ,  Paul Rainville

IPC分类号： H01J49/04 ,  H01J49/26

CPC分类号： H01J49/167 ,  G01N30/7266 ,  H01J49/168

摘要： Described are techniques for tuning parameters of a system. For one or more values of a distance, mass spectral analysis of a liquid analyte stream is performed. A corresponding ion signal intensity of a selected ion is obtained. The distance is measured from a first end of a first tube to a second end of a second tube which surrounds said first tube. Using a computing device, a current value of the distance is automatically adjusted in accordance with corresponding ion signal intensities obtained for the selected ion. Using a computing device, a desired value for the distance is automatically determined using the corresponding ion signal intensities. The desired value results in an ion signal intensity for the selected ion which is any of more than a threshold intensity and a maximum of ion signal intensities obtained by performing mass spectral analysis using different values for the distance.

摘要翻译： 描述了用于调整系统参数的技术。 对于一个或多个距离的值，进行液体分析物流的质谱分析。 获得所选离子的相应离子信号强度。 距离是从第一管的第一端到围绕所述第一管的第二管的第二端测量的。 使用计算设备，根据为所选择的离子获得的对应的离子信号强度，自动调整距离的当前值。 使用计算设备，使用相应的离子信号强度自动确定距离的期望值。 期望的值导致所选择的离子的离子信号强度，其通过使用不同的距离值进行质谱分析而获得的超过阈值强度和离子信号强度的最大值中的任何一个。

公开(公告)号：US10024826B2

公开(公告)日：2018-07-17

申请号：US14238653

申请日：2012-08-22

申请人： James P. Murphy ,  Michael J. Tomany ,  Joseph D. Michienzi ,  Paul Rainville ,  Robert S. Plumb ,  Geoff C. Gerhardt ,  Moon Chul Jung

发明人： James P. Murphy ,  Michael J. Tomany ,  Joseph D. Michienzi ,  Paul Rainville ,  Robert S. Plumb ,  Geoff C. Gerhardt ,  Moon Chul Jung

IPC分类号： G01N30/06 ,  G01N30/16 ,  B01L3/00 ,  G01N30/88 ,  G01N33/49 ,  G01N30/60 ,  G01N30/00 ,  G01N1/38

摘要： An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.

公开(公告)号：US20140047905A1

公开(公告)日：2014-02-20

申请号：US14004240

申请日：2012-03-05

申请人： Michael J. Tomany ,  Paul Rainville ,  James P. Murphy

发明人： Michael J. Tomany ,  Paul Rainville ,  James P. Murphy

IPC分类号： G01N30/72 ,  B05B5/025

CPC分类号： G01N30/72 ,  B05B5/025 ,  B05B5/0255 ,  H01J49/165 ,  Y10T29/49002

摘要： An electrospray emitter assembly (4) for an interface device (1) used to connect the output of a liquid chromatograph to the inlet of an electrospray ion source of a mass spectrometer (10). The device (1) incorporates an electrospray emitter assembly (4) that includes an emitter needle (40), a sheath (6) and a housing (5) with an attachment means in the form of a flange (55) for attachment to a mass spectrometer (10). The sheath (6) is arranged to shield the electrospray emitter needle (40) when the assembly is not attached to a mass spectrometer (10). The assembly (4) is operable to move the sheath (6) thereby to reveal, in use, the electrospray emitter needle (40) when the assembly (4) is attached to the mass spectrometer (10).

摘要翻译： 一种用于将液相色谱仪的输出与质谱仪（10）的电喷雾离子源的入口连接的接口装置（1）的电喷射发射器组件（4）。 装置（1）包括电喷射发射器组件（4），其包括发射针（40），护套（6）和壳体（5），其具有法兰（55）形式的附接装置，用于附接到 质谱仪（10）。 护套（6）布置成当组件未连接到质谱仪（10）时屏蔽电喷射发射器针（40）。 组件（4）可操作以移动护套（6），从而当组件（4）附接到质谱仪（10）时，在使用中显示电喷射发射针（40）。

公开(公告)号：US08598522B2

公开(公告)日：2013-12-03

申请号：US13698336

申请日：2011-05-09

申请人： Michael J. Tomany ,  James P. Murphy ,  Paul Rainville

发明人： Michael J. Tomany ,  James P. Murphy ,  Paul Rainville

IPC分类号： G01N30/72

CPC分类号： H01J49/167 ,  G01N30/7266 ,  H01J49/168

摘要： Described are techniques for tuning parameters of a system. For one or more values of a distance, mass spectral analysis of a liquid analyte stream is performed. A corresponding ion signal intensity of a selected ion is obtained. The distance is measured from a first end of a first tube to a second end of a second tube which surrounds said first tube. Using a computing device, a current value of the distance is automatically adjusted in accordance with corresponding ion signal intensities obtained for the selected ion. Using a computing device, a desired value for the distance is automatically determined using the corresponding ion signal intensities. The desired value results in an ion signal intensity for the selected ion which is any of more than a threshold intensity and a maximum of ion signal intensities obtained by performing mass spectral analysis using different values for the distance.

摘要翻译： 描述了用于调整系统参数的技术。 对于一个或多个距离的值，进行液体分析物流的质谱分析。 获得所选离子的对应离子信号强度。 距离是从第一管的第一端到围绕所述第一管的第二管的第二端测量的。 使用计算设备，根据为所选择的离子获得的对应的离子信号强度，自动调整距离的当前值。 使用计算设备，使用相应的离子信号强度自动确定距离的期望值。 期望的值导致所选择的离子的离子信号强度，其通过使用不同的距离值进行质谱分析而获得的超过阈值强度和离子信号强度的最大值中的任何一个。