公开(公告)号：US07189506B1

公开(公告)日：2007-03-13

申请号：US09518297

申请日：2000-03-03

申请人： Moon Young Lim ,  Cynthia A. Edwards ,  Kirk E. Fry ,  Thomas W. Bruice ,  Douglas B. Starr ,  Megan E. Laurance ,  Yan Kwok ,  Albert W. Tam

发明人： Moon Young Lim ,  Cynthia A. Edwards ,  Kirk E. Fry ,  Thomas W. Bruice ,  Douglas B. Starr ,  Megan E. Laurance ,  Yan Kwok ,  Albert W. Tam

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/90 ,  C07K2319/71 ,  C12N2800/40 ,  C12N2830/001 ,  C12N2830/15

摘要： The present invention provides molecular switch system methods and compositions for use in regulatable gene expression. The system includes a nucleic acid construct which has a DNA response sequence for a transcriptional regulatory protein operably linked to a promoter, a compound binding sequence in the vicinity of the DNA response sequence, a transgene under the control of the promoter; and a DNA binding compound. In some cases, the molecular switch system further includes a nucleic acid sequence encoding a transcriptional regulatory protein operably linked to a second promoter. The invention further provides a method for screening compounds for the ability to function in the molecular switch system and thereby regulate gene expression.

摘要翻译： 本发明提供用于可调节基因表达的分子开关系统方法和组合物。 该系统包括具有与启动子可操作连接的转录调节蛋白的DNA应答序列的核酸构建体，DNA应答序列附近的化合物结合序列，启动子控制下的转基因; 和DNA结合化合物。 在一些情况下，分子交换系统还包括编码与第二启动子可操作地连接的转录调节蛋白的核酸序列。 本发明还提供了一种筛选化合物在分子开关系统中起作用并从而调节基因表达的方法。

公开(公告)号：US06838556B2

公开(公告)日：2005-01-04

申请号：US09875453

申请日：2001-06-06

申请人： Jungsuh P. Kim ,  Douglas B. Starr ,  Albert W. Tam ,  Megan E. Laurance ,  Emil F. Michelotti ,  Mark D. Velligan ,  Derek R. Latour ,  Rita L. Thomas ,  Ana Kongpachith ,  Liana T. Sheppard ,  Moon Young Kim ,  Thomas W. Bruice

发明人： Jungsuh P. Kim ,  Douglas B. Starr ,  Albert W. Tam ,  Megan E. Laurance ,  Emil F. Michelotti ,  Mark D. Velligan ,  Derek R. Latour ,  Rita L. Thomas ,  Ana Kongpachith ,  Liana T. Sheppard ,  Moon Young Kim ,  Thomas W. Bruice

IPC分类号： C12N15/09 ,  C07K14/02 ,  C07K14/315 ,  C07K14/47 ,  C07K14/705 ,  C07K14/72 ,  C07K14/82 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/86 ,  C12Q1/68 ,  C12N15/11

CPC分类号： C07K14/005 ,  C07K14/315 ,  C07K14/4738 ,  C07K14/70578 ,  C07K14/72 ,  C07K14/82 ,  C12N9/86 ,  C12N2730/10122

摘要： This invention provides nucleic acid sequences, vectors and host cells comprising regulatory regions associated with various promoters including a cyclin D1 promoter, a CD40L promoter, three HBV promoters (core, pre-S1 and HBV-X), a vancomycin-resistant enterococci (VRE) promoter, an androgen receptor promoter, a Her2 promoter, and β-lactamase promoter. The invention further provides methods of regulating gene expression comprising the regulatory regions of such promoters.

公开(公告)号：US07094406B2

公开(公告)日：2006-08-22

申请号：US09851410

申请日：2001-05-07

申请人： Gregory R. Reyes ,  Patrice O. Yarbough ,  Daniel W. Bradley ,  Krzysztof Z. Krawczynski ,  Albert W. Tam ,  Kirk E. Fry

发明人： Gregory R. Reyes ,  Patrice O. Yarbough ,  Daniel W. Bradley ,  Krzysztof Z. Krawczynski ,  Albert W. Tam ,  Kirk E. Fry

IPC分类号： A61K39/29 ,  C07K4/02 ,  C07K7/00 ,  C07K14/08

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K16/10 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2760/10122 ,  C12N2770/00022 ,  C12N2770/24222 ,  C12N2770/28122 ,  C12Q1/707 ,  G01N33/5767 ,  Y10T436/143333

摘要： Viral proteins derived from an enterically transmitted non-A/non-B viral hepatitis agent (HEV) are disclosed. In one embodiment, the protein is immunologically reactive with antibodies present in individuals infected with the viral hepatitis agent. This protein is useful in a diagnostic method for detecting infection by the enterically transmitted agent. Specific epitopes have been identified that are reactive with sera of individual infected with different strains of HEV. Also disclosed are DNA probes derived from a cloned sequence of the viral agent. These probes are useful for identifying and sequencing the entire viral agent and for assaying the presence of the viral agent in an infected sample, by using probe-specific amplification of virus-derived DNA fragments.

公开(公告)号：US06229005B1

公开(公告)日：2001-05-08

申请号：US09128275

申请日：1998-08-03

申请人： Gregory R. Reyes ,  Patrice O. Yarbough ,  Daniel W. Bradley ,  Krzysztof Z. Krawczynski ,  Albert W. Tam ,  Kirk E. Fry

发明人： Gregory R. Reyes ,  Patrice O. Yarbough ,  Daniel W. Bradley ,  Krzysztof Z. Krawczynski ,  Albert W. Tam ,  Kirk E. Fry

IPC分类号： C07H2104

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K16/10 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2760/10122 ,  C12N2770/00022 ,  C12N2770/24222 ,  C12N2770/28122 ,  C12Q1/707 ,  G01N33/5767 ,  Y10T436/143333

摘要： Viral proteins derived from an enterically transmitted non-A/non-B viral hepatitis agent (HEV) are disclosed. In one embodiment, the protein is immunologically reactive with antibodies present in individuals infected with the viral hepatitis agent. This protein is useful in a diagnostic method for detecting infection by the enterically transmitted agent. Specific epitopes have been identified that are reactive with sera of individual infected with different strains of HEV. Also disclosed are DNA probes derived from a cloned sequence of the viral agent. These probes are useful for identifying and sequencing the entire viral agent and for assaying the presence of the viral agent in an infected sample, by using probe-specific amplification of virus-derived DNA fragments.

摘要翻译： 公开了从肠道传播的非A /非B病毒性肝炎病毒（HEV）衍生的病毒蛋白。 在一个实施方案中，所述蛋白质与存在于感染病毒性肝炎病毒的个体中的抗体具有免疫反应性。 该蛋白质可用于检测肠道传播物质感染的诊断方法。 已经鉴定出与不同HEV病毒株感染的个体血清反应的特异性表位。 还公开了衍生自病毒剂的克隆序列的DNA探针。 这些探针可用于通过使用病毒衍生的DNA片段的探针特异性扩增来鉴定和测序整个病毒剂并测定感染样品中病毒剂的存在。

公开(公告)号：US5885768A

公开(公告)日：1999-03-23

申请号：US876941

申请日：1992-05-01

申请人： Gregory R. Reyes ,  Daniel W. Bradley ,  Albert W. Tam ,  Mitchell Carl

发明人： Gregory R. Reyes ,  Daniel W. Bradley ,  Albert W. Tam ,  Mitchell Carl

IPC分类号： G01N15/00 ,  A61K38/00 ,  A61K39/00 ,  A61K39/29 ,  A61P31/12 ,  A61P37/04 ,  C07K7/08 ,  C07K14/08 ,  C07K16/10 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/70 ,  C12R1/19 ,  G01N33/576

CPC分类号： C07K14/005 ,  C07K16/10 ,  G01N33/5767 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2770/28122

摘要： Immunogenic peptides derived from the ORF1, ORF2, and ORF3 regions of hepatitis E virus (HEV), diagnostic reagents containing the peptide antigens, vaccine compositions containing the antigens, and antibodies which are immunoreactive with the antigens are disclosed.

摘要翻译： 公开了衍生自戊型肝炎病毒（HEV）的ORF1，ORF2和ORF3区域的免疫原性肽，含有肽抗原的诊断试剂，含有抗原的疫苗组合物和与抗原免疫反应的抗体。

公开(公告)号：US5686239A

公开(公告)日：1997-11-11

申请号：US240049

申请日：1994-05-09

申请人： Gregory R. Reyes ,  Albert W. Tam ,  Patrice O. Yarbough

发明人： Gregory R. Reyes ,  Albert W. Tam ,  Patrice O. Yarbough

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/08 ,  C07K16/10 ,  C12Q1/70 ,  C07K14/005 ,  C07K17/00 ,  G01N33/543

CPC分类号： C07K14/005 ,  C07K16/10 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2770/28122 ,  Y10S435/975

摘要： Peptide antigens are provided which are derived from the enterically transmitted non-A/non-B viral hepatitis agent, known as hepatitis E virus (HEV). The HEV derived peptides and in particular, SG3, are immunoreactive with sera from individuals infected with HEV. The antigens are useful as diagnostic reagents in diagnostic methods and kits for determining infection of an individual with HEV.

摘要翻译： 提供肽抗原，其衍生自称为戊型肝炎病毒（HEV）的肠道传播的非A /非B型病毒性肝炎病毒。 HEV衍生的肽，特别是SG3与来自感染HEV的个体的血清具有免疫反应性。 抗原在诊断方法和试剂盒中可用作诊断试剂，用于确定HEV感染个体。

公开(公告)号：US06455492B1

公开(公告)日：2002-09-24

申请号：US07870985

申请日：1992-04-20

申请人： Gregory R. Reyes ,  Daniel W. Bradley ,  Jr-Shin Twu ,  Michael A. Purdy ,  Albert W. Tam ,  Krzysztof Z. Krawczynski ,  Patrice O. Yarbough

发明人： Gregory R. Reyes ,  Daniel W. Bradley ,  Jr-Shin Twu ,  Michael A. Purdy ,  Albert W. Tam ,  Krzysztof Z. Krawczynski ,  Patrice O. Yarbough

IPC分类号： A01N3718

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K16/10 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2770/28022 ,  C12N2770/28122 ,  G01N33/5767

摘要： Antigen and antibody vaccine composition effective in preventing hepatitis E virus (HEV) infection are disclosed. The antigen composition includes peptides corresponding to carboxyl terminal end regions of the second and third open reasing frames of the HEV genome. The composition is effective in preventing HEV infection after vaccination. The antibody composition contains an antibody effective to block HEV infection of human primary hepatocytes in culture.

摘要翻译： 公开了有效预防戊型肝炎病毒（HEV）感染的抗原和抗体疫苗组合物。 抗原组合物包括对应于HEV基因组的第二和第三开放载体框架的羧基末端区域的肽。 该组合物在接种疫苗后有效预防HEV感染。 抗体组合物含有有效阻断培养物中人原代肝细胞HEV感染的抗体。

公开(公告)号：US5770689A

公开(公告)日：1998-06-23

申请号：US484054

申请日：1995-06-07

申请人： Gregory R. Reyes ,  Daniel W. Bradley ,  Jr-Shin Twu ,  Michael A. Purdy ,  Albert W. Tam ,  Krzysztof Z. Krawczynski ,  Patrice O. Yarbough

发明人： Gregory R. Reyes ,  Daniel W. Bradley ,  Jr-Shin Twu ,  Michael A. Purdy ,  Albert W. Tam ,  Krzysztof Z. Krawczynski ,  Patrice O. Yarbough

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K39/00 ,  A61K39/29 ,  A61K39/395 ,  A61P31/12 ,  C07K14/08 ,  C07K16/10 ,  C12N15/51 ,  C12P21/08 ,  C12Q1/70 ,  G01N33/576 ,  C07K5/00

CPC分类号： C07K14/005 ,  C07K16/10 ,  G01N33/5767 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2770/28022 ,  C12N2770/28122

摘要： An antigen composition hepatitis E virus (HEV) infection are disclosed. The antigen composition includes peptides corresponding to carboxyl terminal end regions of the second and third open reading frames of the HEV genome.

摘要翻译： 公开了一种抗原组合物戊型肝炎病毒（HEV）感染。 抗原组合物包括对应于HEV基因组的第二和第三开放阅读框的羧基末端区的肽。

公开(公告)号：US5741490A

公开(公告)日：1998-04-21

申请号：US259148

申请日：1994-06-13

申请人： Gregory R. Reyes ,  Daniel W. Bradley ,  Jr-Shin Twu ,  Michael A. Purdy ,  Albert W. Tam ,  Krzysztof Z. Krawczynski ,  Patrice D. Yarbough

发明人： Gregory R. Reyes ,  Daniel W. Bradley ,  Jr-Shin Twu ,  Michael A. Purdy ,  Albert W. Tam ,  Krzysztof Z. Krawczynski ,  Patrice D. Yarbough

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/08 ,  C07K16/10 ,  A61K39/29

CPC分类号： C07K14/005 ,  C07K16/10 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2770/28122

摘要： Antigen and antibody vaccine composition effective in preventing hepatitis E virus (HEV) infection are disclosed. The antigen composition includes a peptide corresponding to a carboxyl terminal end region of the capsid protein encoded by the second open reading frame 2 of the HEV genome. The composition is effective in preventing HEV infection after vaccination. The antibody composition contains an antibody effective to block HEV infection of human primary hepatocytes in culture.