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公开(公告)号：US07968318B2
公开(公告)日：2011-06-28
申请号：US11447554
申请日：2006-06-06
申请人： Oreste J. Lantero , Mian Li , Jayarama K. Shetty
发明人： Oreste J. Lantero , Mian Li , Jayarama K. Shetty
摘要： A method is disclosed for producing glucose from a granular starch substrate including, contacting a slurry comprising granular starch obtained from plant material with an alpha-amylase at a temperature below the starch gelatinization temperature of the granular starch to produce oligosaccharides and hydrolyzing the oligosaccharides to produce a mash comprising at least 20% glucose and further comprising fermenting the mash to obtain ethanol.
摘要翻译： 公开了一种用于从颗粒淀粉基质生产葡萄糖的方法，包括在低于淀粉糊化温度的淀粉糊化温度的温度下使包含从植物材料获得的颗粒淀粉与α-淀粉酶接触的浆料接触以产生寡糖并水解寡糖以产生 含有至少20％葡萄糖的醪液，还包括发酵所述糊状物以获得乙醇。
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公开(公告)号：US20110223639A1
公开(公告)日：2011-09-15
申请号：US13113516
申请日：2011-05-23
申请人： Oreste J. LANTERO , Mian Li , Jayarama K. Shetty
发明人： Oreste J. LANTERO , Mian Li , Jayarama K. Shetty
摘要： The present invention concerns a method of producing glucose from a granular starch substrate comprising, contacting a slurry comprising granular starch obtained from plant material with an alpha-amylase at a temperature below the starch gelatinization temperature of the granular starch to produce oligosaccharides and hydrolyzing the oligosaccharides to produce a mash comprising at least 20% glucose and further comprising fermenting the mash to obtain ethanol.
摘要翻译： 本发明涉及一种从颗粒状淀粉基质制备葡萄糖的方法，包括：在低于淀粉糊化温度的淀粉糊化温度的温度下将包含从植物材料获得的颗粒状淀粉与α-淀粉酶接触的浆液接触以产生寡糖并水解低聚糖 以产生包含至少20％葡萄糖的醪液，并进一步包括发酵所述醪液以获得乙醇。
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公开(公告)号：US20070281344A1
公开(公告)日：2007-12-06
申请号：US11447554
申请日：2006-06-06
申请人： Oreste J. Lantero , Mian Li , Jayarama K. Shetty
发明人： Oreste J. Lantero , Mian Li , Jayarama K. Shetty
摘要： The present invention concerns a method of producing glucose from a granular starch substrate comprising, contacting a slurry comprising granular starch obtained from plant material with an alpha-amylase at a temperature below the starch gelatinization temperature of the granular starch to produce oligosaccharides and hydrolyzing the oligosaccharides to produce a mash comprising at least 20% glucose and further comprising fermenting the mash to obtain ethanol.
摘要翻译： 本发明涉及一种从颗粒状淀粉基质制备葡萄糖的方法，包括：在低于淀粉糊化温度的淀粉糊化温度的温度下将包含从植物材料获得的颗粒状淀粉与α-淀粉酶接触的浆液接触以产生寡糖并水解低聚糖 以产生包含至少20％葡萄糖的醪液，并进一步包括发酵所述醪液以获得乙醇。
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4.发明授权Enzyme compositions comprising a glucoamylase, an acid stable alpha amylase, and an acid fungal protease 有权包含葡糖淀粉酶，酸稳定的α淀粉酶和酸性真菌蛋白酶的酶组合物公开(公告)号：US08048657B2
公开(公告)日：2011-11-01
申请号：US12250789
申请日：2008-10-14
摘要： The present invention relates to an enzyme blend composition comprising a glucoamylase, an acid stable alpha amylase, and an acid fungal protease. The present invention is further directed to a method for producing end products such as alcohols from fermentable sugars, comprising the steps of: (a) contacting a slurry comprising a milled grain that contains starch with an alpha amylase to produce a liquefact; (b) contacting the liquefact with a glucoamylase, an acid stable alpha amylase, and an acid fungal protease, to produce fermentable sugars; and (c) fermenting the fermentable sugars in the presence of a fermenting organism to produce end products.
摘要翻译： 本发明涉及包含葡糖淀粉酶，酸稳定的α淀粉酶和酸性真菌蛋白酶的酶混合组合物。 本发明还涉及从可发酵糖生产最终产品如醇的方法，包括以下步骤：（a）使含有淀粉的研磨颗粒与α-淀粉酶的浆料接触以产生液化; （b）使液化酶与葡糖淀粉酶，酸稳定的α淀粉酶和酸性真菌蛋白酶接触以产生可发酵的糖; 和（c）在发酵生物存在下发酵可发酵糖以产生最终产物。
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5.发明授权公开(公告)号：US08323932B2
公开(公告)日：2012-12-04
申请号：US13230707
申请日：2011-09-12
申请人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
发明人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
IPC分类号： C12P13/04 , C12P13/24 , C12P13/22 , C12P13/20 , C12P19/14 , C12P19/20 , C12P19/02 , C12N9/32 , C12N9/34 , C07H21/04
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。
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6.发明授权公开(公告)号：US08034590B2
公开(公告)日：2011-10-11
申请号：US11875279
申请日：2007-10-19
申请人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
发明人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。
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公开(公告)号：US20090203101A1
公开(公告)日：2009-08-13
申请号：US12250789
申请日：2008-10-14
摘要： The present invention relates to an enzyme blend composition comprising a glucoamylase, an acid stable alpha amylase, and an acid fungal protease. The present invention is further directed to a method for producing end products such as alcohols from fermentable sugars, comprising the steps of: (a) contacting a slurry comprising a milled grain that contains starch with an alpha amylase to produce a liquefact; (b) contacting the liquefact with a glucoamylase, an acid stable alpha amylase, and an acid fungal protease, to produce fermentable sugars; and (c) fermenting the fermentable sugars in the presence of a fermenting organism to produce end products.
摘要翻译： 本发明涉及包含葡糖淀粉酶，酸稳定的α淀粉酶和酸性真菌蛋白酶的酶混合组合物。 本发明还涉及从可发酵糖生产最终产品如醇的方法，包括以下步骤：（a）使含有淀粉的研磨颗粒与α-淀粉酶的浆料接触以产生液化; （b）使液化酶与葡糖淀粉酶，酸稳定的α淀粉酶和酸性真菌蛋白酶接触以产生可发酵的糖; 和（c）在发酵生物存在下发酵可发酵糖以产生最终产物。
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8.发明申请Expression of Granular Starch Hydrolyzing Enzymes in Trichoderma and process for producing glucose from granular starch substrates 有权颗粒状淀粉水解酶在木霉菌中的表达及由颗粒淀粉基质生产葡萄糖的方法公开(公告)号：US20090203087A1
公开(公告)日：2009-08-13
申请号：US11875279
申请日：2007-10-19
申请人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, JR. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
发明人： Toby M. Baldwin , Benjamin S. Bower , Gopal K. Chotani , Nigel Dunn-Coleman , Oreste J. Lantero, JR. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty , Bruce A. Strohm
CPC分类号： C12P19/14 , C12N1/14 , C12N9/242 , C12N9/2428 , C12P19/02 , C12P19/04 , C12P19/20 , C12P19/24 , Y02E50/17 , Y02P20/52
摘要： The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.
摘要翻译： 本发明涉及丝状真菌宿主细胞，特别涉及可用于生产具有葡糖淀粉酶活性的异源粒状淀粉水解酶（GSHE）的木霉宿主细胞。 此外，本发明涉及一种葡萄糖浆的制造方法，其特征在于，使粒状淀粉底物获得的粒状淀粉浆料与α淀粉酶和GSHE同时等于或低于所述粒状淀粉的凝胶化温度，得到组合物 的葡萄糖浆。
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9.发明授权公开(公告)号：US07037704B2
公开(公告)日：2006-05-02
申请号：US10999886
申请日：2004-11-30
申请人： Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty
发明人： Nigel Dunn-Coleman , Oreste J. Lantero, Jr. , Suzanne E. Lantz , Michael J. Pepsin , Jayarama K. Shetty
CPC分类号： C12P7/06 , C12N9/242 , C12N9/2428 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25–40° C. to obtain a fermentation broth having 5 to 20% ethanol.
摘要翻译： 本发明涉及含有具有淀粉淀粉水解活性的酸稳定性α淀粉酶（asAA）的粒状淀粉水解酶组合物。 本发明还涉及一种生产醇的一步法，该方法包括在发酵步骤中使颗粒淀粉基质与具有颗粒状淀粉水解（GSH）活性的酸稳定性α淀粉酶（asAA）和葡糖淀粉酶（GA）接触， 还在25-40℃的温度下包含产乙醇微生物以获得具有5至20％乙醇的发酵液。
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10.发明授权Method for the treatment of sticky cotton fiber with transglucosidase from Aspergillus niger 失效用来自黑曲霉的转葡糖苷酶处理粘性棉纤维的方法
公开(公告)号：US5516689A
公开(公告)日：1996-05-14
申请号：US54226
申请日：1993-04-30
IPC分类号： C12N9/10 , C12N9/30 , C12P19/14 , C12R1/685 , C12S11/00 , D01C1/00 , D06L1/00 , D06L1/12 , D06M16/00 , D21C1/00
CPC分类号： D06L1/00 , D01C1/00 , D06L1/12 , D06M16/003 , D06M2101/06
摘要： An enzyme composition and a means of reducing the stickiness of honeydew contaminated cotton is disclosed. The composition includes, and the method uses, enzymes such as transglucosidases and pectinases which are capable of hydrolyzing sugars that make-up honeydew. Suitable transglucosidases include those obtained from Aspergillus niger. Particularly preferred is a transglucosidase obtained from Aspergillus niger ATCC 14916.
摘要翻译： 公开了一种酶组合物和降低蜜汁污染棉的粘性的方法。 该组合物包括并且该方法使用能够水解构成蜜汁的糖的酶如转葡糖苷酶和果胶酶。 合适的转葡糖苷酶包括从黑曲霉获得的那些。 特别优选的是从黑曲霉ATCC 14916获得的转葡糖苷酶。
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