公开(公告)号：US08252605B2

公开(公告)日：2012-08-28

申请号：US12431575

申请日：2009-04-28

Applicant: Roland Janzen ,  Carsten Ulrich Schelp

Inventor： Roland Janzen ,  Carsten Ulrich Schelp

IPC: G01N33/543

CPC classification number: G01N33/54333

Abstract: The invention relates to methods and compositions for removing a dissociated species from a fluid medium solution during and after it has detached from a solid-phase immersed in said medium, thereby allowing the concentration of free species to remain close to zero, and for improving the signal to noise ratio in assays. This is achieved by employing a substrate, such as a scavenging solid-phase, having an attached binding partner or partners (“scavenger”) for the specifically binding species and which is present during storage. This substrate may also contain regions for binding signal generating components attached to the solid-phase. This substrate binds any free species bleeding off the solid phase, increasing the reliability and sensitivity of assays. A subset of the substrates in the invention additionally forms cross-linked networks of solid-phase particles that further increase the sensitivity of assays.

Abstract translation: 本发明涉及用于在液体介质溶液从浸入所述介质中的固相脱离时从流体介质溶液中除去离解物质的方法和组合物，从而允许游离物质的浓度保持接近零，并且为了改善 测定中的信噪比。 这通过使用具有附着的结合配偶体或伴侣（“清道夫”）的底物，例如清除固相，并且在储存期间存在。 该衬底还可以包含用于结合连接到固相的信号产生部件的区域。 该底物结合任何自由物质脱离固相，增加了测定的可靠性和灵敏度。 本发明的底物的一个子集另外形成进一步提高测定灵敏度的固相颗粒的交联网络。

公开(公告)号：US20090208987A1

公开(公告)日：2009-08-20

申请号：US12431575

申请日：2009-04-28

Applicant: Roland Janzen ,  Carsten Ulrich Schelp

Inventor： Roland Janzen ,  Carsten Ulrich Schelp

IPC: G01N33/566

CPC classification number: G01N33/54333

Abstract: The invention relates to methods and compositions for removing a dissociated species from a fluid medium solution during and after it has detached from a solid-phase immersed in said medium, thereby allowing the concentration of free species to remain close to zero, and for improving the signal to noise ratio in assays. This is achieved by employing a substrate, such as a scavenging solid-phase, having an attached binding partner or partners (“scavenger”) for the specifically binding species and which is present during storage. This substrate may also contain regions for binding signal generating components attached to the solid-phase. This substrate binds any free species bleeding off the solid phase, increasing the reliability and sensitivity of assays. A subset of the substrates in the invention additionally forms cross-linked networks of solid-phase particles that further increase the sensitivity of assays.

Abstract translation: 本发明涉及用于在液体介质溶液从浸入所述介质中的固相脱离时从流体介质溶液中除去离解物质的方法和组合物，从而允许游离物质的浓度保持接近零，并且为了改善 测定中的信噪比。 这通过使用具有附着的结合配偶体或伴侣（“清道夫”）的底物，例如清除固相，并且在储存期间存在。 该衬底还可以包含用于结合连接到固相的信号产生部件的区域。 该底物结合任何自由物质脱离固相，增加了测定的可靠性和灵敏度。 本发明的底物的一个子集另外形成进一步提高测定灵敏度的固相颗粒的交联网络。

公开(公告)号：US09244083B2

公开(公告)日：2016-01-26

申请号：US13690332

申请日：2012-11-30

Applicant: Zhu Teng ,  Roland Janzen ,  Martin A. Drinan

Inventor： Zhu Teng ,  Roland Janzen ,  Martin A. Drinan

IPC: G01N33/532 ,  G01N33/82 ,  C07C401/00 ,  A61K39/385

CPC classification number: G01N33/82 ,  A61K39/385 ,  C07C401/00 ,  C07F5/003 ,  G01N21/76 ,  G01N33/532 ,  Y10T436/203332

Abstract: Compounds include carbamate derivatives of vitamin D including vitamin D3and vitamin D2. The compounds are useful in methods and kits for determining the presence and/or amount of vitamin D including vitamin D analogs and metabolites thereof in a sample suspected of containing the same.

Abstract translation: 化合物包括维生素D的氨基甲酸酯衍生物，包括维生素D3和维生素D2。 该化合物可用于在怀疑含有其的样品中测定维生素D（包括维生素D类似物及其代谢物）的存在和/或量的存在和/或量的方法和试剂盒。

公开(公告)号：US20140212987A1

公开(公告)日：2014-07-31

申请号：US13753909

申请日：2013-01-30

Applicant: Pratap Singh ,  Roland Janzen ,  Jie Li ,  Nina Zolotarjova

Inventor： Pratap Singh ,  Roland Janzen ,  Jie Li ,  Nina Zolotarjova

IPC: G01N33/82

CPC classification number: G01N33/82 ,  G01N33/5306 ,  G01N33/6854

Abstract: Methods of enhancing signal ratio between calibrators in an assay for an analyte include conducting an assay for the analyte with zero concentration of analyte in a first calibrator to determine a first signal level. The reagents employed in the assay comprise an antibody reagent comprising an antibody for the analyte wherein a hinge region of the antibody is conjugated to a moiety. The assay for the analyte is also conducted with a second concentration of analyte in a second calibrator to determine a second signal level wherein the second analyte concentration is greater than zero and wherein the reagents employed in the assay comprise the antibody reagent. A ratio of the first signal level to the second signal level is determined and evaluated.

Abstract translation: 在分析物测定中增强校准器之间的信号比率的方法包括在第一校准器中对零分析物进行分析物测定以确定第一信号水平。 在测定中使用的试剂包含抗体试剂，其包含用于分析物的抗体，其中抗体的铰链区域与部分缀合。 分析物的测定还用第二校准器中的第二浓度的分析物进行，以确定第二分析物浓度大于零的第二信号水平，并且其中测定中使用的试剂包含抗体试剂。 确定并评估第一信号电平与第二信号电平的比率。

公开(公告)号：US09347947B2

公开(公告)日：2016-05-24

申请号：US12403299

申请日：2009-03-12

Applicant: Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

Inventor： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

IPC: G01N21/76 ,  G01N33/58

CPC classification number: G01N33/78 ,  G01N33/582 ,  G01N2333/59

Abstract: Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

Abstract translation: 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 该组合经受用于将分析物与分析物的结合配偶体结合的条件。 敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

公开(公告)号：US20140154700A1

公开(公告)日：2014-06-05

申请号：US13690332

申请日：2012-11-30

Applicant: Zhu Teng ,  Roland Janzen ,  Martin A. Drinan

Inventor： Zhu Teng ,  Roland Janzen ,  Martin A. Drinan

IPC: G01N33/82

CPC classification number: G01N33/82 ,  A61K39/385 ,  C07C401/00 ,  C07F5/003 ,  G01N21/76 ,  G01N33/532 ,  Y10T436/203332

Abstract: Compounds include carbamate derivatives of vitamin D including vitamin D3 and vitamin D2. The compounds are useful in methods and kits for determining the presence and/or amount of vitamin D including vitamin D analogs and metabolites thereof in a sample suspected of containing the same.

Abstract translation: 化合物包括维生素D的氨基甲酸酯衍生物，包括维生素D3和维生素D2。 该化合物可用于在怀疑含有其的样品中测定维生素D（包括维生素D类似物及其代谢物）的存在和/或量的存在和/或量的方法和试剂盒。

公开(公告)号：US08723134B2

公开(公告)日：2014-05-13

申请号：US12914252

申请日：2010-10-28

Applicant: Roland Janzen

Inventor： Roland Janzen

IPC: H01J37/153

CPC classification number: H01J37/153 ,  B82Y10/00 ,  B82Y40/00 ,  H01J37/12 ,  H01J37/26 ,  H01J37/3177 ,  H01J2237/1205 ,  H01J2237/121 ,  H01J2237/1516 ,  H01J2237/1532

Abstract: A correction device for a charged particle beam device for decreasing, correcting or inverting (that is adjusting) the spherical aberration of a charged particle beam is described. The correction principle is similar to that of common multipole-Correctors. But unlike common devices of that kind this new correction device gets along entirely with plane apertures having specially shaped holes in order to supply the multipoles required for correction and is therefore predestined for miniaturization and the use in multi column devices.

Abstract translation: 描述了用于减少，校正或反转（即调整）带电粒子束的球面像差的带电粒子束装置的校正装置。 校正原理与普通多极校正器相似。 但是与这种常见的装置不同，这种新的校正装置完全与具有特殊形状的孔的平面孔相交，以便提供校正所需的多极，因此被预定为用于小型化和在多列装置中的使用。

公开(公告)号：US20100233725A1

公开(公告)日：2010-09-16

申请号：US12403299

申请日：2009-03-12

Applicant: Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

Inventor： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

IPC: G01N33/53

CPC classification number: G01N33/78 ,  G01N33/582 ,  G01N2333/59

Abstract: Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

Abstract translation: 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 该组合经受用于将分析物与分析物的结合配偶体结合的条件。 敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

公开(公告)号：US20050112586A1

公开(公告)日：2005-05-26

申请号：US10720909

申请日：2003-11-24

Applicant: Roland Janzen ,  Carsten Schelp

Inventor： Roland Janzen ,  Carsten Schelp

IPC: C12Q1/00 ,  C12Q1/68 ,  G01N33/543

CPC classification number: G01N33/54333

Abstract: The invention relates to methods and compositions for removing a dissociated species from a fluid medium solution during and after it has detached from a solid-phase immersed in said medium, thereby allowing the concentration of free species to remain close to zero, and for improving the signal to noise ratio in assays. This is achieved by employing a substrate, such as a scavenging solid-phase, having an attached binding partner or partners (“scavenger”) for the specifically binding species and which is present during storage. This substrate may also contain regions for binding signal generating components attached to the solid-phase. This substrate binds any free species bleeding off the solid phase, increasing the reliability and sensitivity of assays. A subset of the substrates in the invention additionally forms cross-linked networks of solid-phase particles that further increase the sensitivity of assays.

Abstract translation: 本发明涉及用于在液体介质溶液从浸入所述介质中的固相脱离时从流体介质溶液中除去离解物质的方法和组合物，从而允许游离物质的浓度保持接近零，并且为了改善 测定中的信噪比。 这通过使用具有附着的结合配偶体或伴侣（“清道夫”）的底物，例如清除固相，并且在储存期间存在。 该衬底还可以包含用于结合连接到固相的信号产生部件的区域。 该底物结合任何自由物质脱离固相，增加了测定的可靠性和灵敏度。 本发明的底物的一个子集另外形成进一步提高测定灵敏度的固相颗粒的交联网络。

公开(公告)号：US06774372B1

公开(公告)日：2004-08-10

申请号：US10018904

申请日：2002-01-15

Applicant: Harald Rose ,  Peter Schmid ,  Roland Janzen

Inventor： Harald Rose ,  Peter Schmid ,  Roland Janzen

IPC: H01J3726

CPC classification number: B82Y10/00 ,  B82Y40/00 ,  H01J37/3007 ,  H01J37/3174

Abstract: An electron-optical lens arrangement with an axis that can be substantially displaced, and useful for electron lithography, includes a cylinder lens and a quadrupole field. The plane of symmetry of the quadrupole field extends in the mid-plane of the gap pertaining to the cylinder lens. The focussing level of the quadrupole is oriented in the direction of the gap. The amount of the focussing refractive power belonging to the cylinder lens is twice as high as the amount of the quadrupole. A deflection system for the charged particles is connected upstream in the level of the gap pertaining to the cylinder lens and several electrodes or pole shoes, which generate a quadrupole field are provided in the direction of the gap pertaining to the cylinder lens. The electrodes or pole shoes can be individually and, preferably, successively excited and the quadrupole field can be displaced according to the deflection of the particle beam, so that the particle beam impinges upon the area of the quadrupole field. A holding device is provided for an object, such as a wafer, and is arranged vertically in relation to the optical axis and can be displaced in relation to the direction of the gap pertaining to the cylinder lens.

Abstract translation: 可以基本上位移并且可用于电子光刻的电子 - 光学透镜装置包括柱面透镜和四极场。 四极场的对称平面在与柱面透镜相关的间隙的中间平面内延伸。 四极杆的聚焦位置取向于间隙的方向。 属于圆柱透镜的聚焦屈光力的量是四极的量的两倍。 带电粒子的偏转系统在与气缸透镜相关的间隙的水平面上游连接，并且产生四极场的几个电极或极靴在与气缸透镜相关的间隙的方向上设置。 电极或极靴可以单独地，优选地被连续地激发，并且四极场可以根据粒子束的偏转而移位，使得粒子束撞击四极场的区域。 为诸如晶片的物体提供保持装置，并且相对于光轴垂直地布置，并且可以相对于与柱面透镜相关的间隙的方向移位。