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    PLANT-BIOMASS HYDROLYSIS METHOD
    2.
    发明申请
    PLANT-BIOMASS HYDROLYSIS METHOD 审中-公开
    植物生物量水解法

    公开(公告)号：US20150337403A1

    公开(公告)日：2015-11-26

    申请号：US14653139

    申请日：2013-11-19

    Applicant: SHOWA DENKO K.K. ,  NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY

    Inventor： Ichiro FUJITA ,  Tadashi YONEDA ,  Atsushi FUKUOKA ,  Hirokazu KOBAYASHI ,  Mizuho YABUSHITA

    IPC: C13K1/02

    CPC classification number: C13K1/02

    Abstract: A method for hydrolyzing a plant biomass, which includes hydrothermal treatment in the presence of an equivalent concentration of an acid equal to 30 to 1,000% of the equivalent concentration of cations in a plant-biomass hydrolysis reaction solution; and a method for producing glucose using the above method for hydrolyzing a plant biomass. The hydrothermal treatment is preferably conducted by using a solid catalyst comprising a carbon material and using inorganic acid as acid. The method eliminates reaction-inhibiting factors to thereby obtain a high glucose yield.

    Abstract translation: 一种水解植物生物质的方法，其包括在等效浓度相当于植物 - 生物质水解反应溶液中当量阳离子浓度的30至1,000％的酸的存在下的水热处理; 以及使用上述水解植物生物量的方法生产葡萄糖的方法。 水热处理优选通过使用包含碳材料并使用无机酸作为酸的固体催化剂进行。 该方法消除了反应抑制因子，从而获得高葡萄糖产率。

    METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS
    4.
    发明申请
    METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS 审中-公开
    用于处理含细菌生物质的方法

    公开(公告)号：US20160251690A1

    公开(公告)日：2016-09-01

    申请号：US15027617

    申请日：2014-09-09

    Applicant: SHOWA DENKO K.K.

    Inventor： Ichiro FUJITA ,  Shinji YAMAKI ,  Tadashi YONEDA

    IPC: C12P19/14 ,  C12P19/02

    CPC classification number: C12P19/14 ,  C12M45/02 ,  C12P19/02 ,  C12P2201/00 ,  C13K1/02

    Abstract: A treatment method for biomass, which is a pretreatment method including continuously performing hydrothermal treatment using a screw extruder to produce a biomass composition for saccharification from cellulose-containing biomass and containing only a small quantity of furfural, the treatment method sequentially including: pulverizing the cellulose-containing biomass so as to have a maximum grain size of 1,000 μm or less and adjusting a water content ratio thereof to from 30% to 80%; performing hydrothermal treatment in which an element including a seal ring and at least one set of a kneading disc and/or a left-hand screw is arranged immediately upstream of the seal ring, at a temperature of (1) from 150° C. to 200° C. or (2) from 200° C. to 215° C. for 0.1 minute to 15 minutes while performing kneading pulverization having a grinding effect; and cooling a treated product to 100° C. or less to recover the treated product.

    Abstract translation: 一种生物质处理方法，其特征在于，所述预处理方法包括使用螺杆挤出机连续进行水热处理，以生产由含纤维素生物质糖化并仅含有少量糠醛的生物质组合物，所述处理方法依次包括：将所述纤维素 含有生物质，使其最大粒径为1000μm以下，将其含水率调整为30〜80％。 进行水热处理，其中包括密封环和至少一组捏合盘和/或左旋螺杆的元件紧邻密封环的上游，在（1）从150℃到 200℃或（2）从200℃至215℃进行0.1分钟至15分钟，同时进行具有研磨效果的捏合粉碎; 并将处理的产物冷却至100℃或更低以回收经处理的产物。

    METHOD FOR ASSAYING CELLULASE ACTIVITY, SCREENING METHOD USING ASSAYING METHOD, AND HIGH-PERFORMANCE CELLULASE-PRODUCING BACTERIA SELECTED USING SCREENING METHOD
    5.
    发明申请
    METHOD FOR ASSAYING CELLULASE ACTIVITY, SCREENING METHOD USING ASSAYING METHOD, AND HIGH-PERFORMANCE CELLULASE-PRODUCING BACTERIA SELECTED USING SCREENING METHOD 审中-公开
    用于测定纤维素酶活性的方法，使用测定方法的筛选方法和使用筛选方法选择的高性能纤维素酶生产细菌

    公开(公告)号：US20160244804A1

    公开(公告)日：2016-08-25

    申请号：US15044691

    申请日：2016-02-16

    Applicant: SHOWA DENKO K.K.

    Inventor： Ichiro FUJITA ,  Shinji YAMAKI ,  Tadashi YONEDA

    IPC: C12Q1/34 ,  C12N1/14 ,  C12R1/645

    CPC classification number: C12Q1/34 ,  C12R1/645 ,  G01N2333/942

    Abstract: A method for assaying cellulase activity, including: a process (A) of preparing two or more substrate solutions, which have an identical absorbance measured at an identical wavelength and in which cellulose is dispersed at an identical concentration, and measuring the absorbance of each of the substrate solutions; a process (B) of respectively adding different kinds of enzyme solutions to the substrate solutions, and performing an enzyme reaction under the same conditions; a process (C) of measuring the absorbance of each of the substrate solutions after the enzyme reaction; a process (D) of calculating the absorbance decrease values of the substrate solutions before and after the enzyme reaction; and a process (E) of assaying cellulase activities of the enzyme solutions based on the absorbance decrease values. In the assay of the process (E), it is determined that the enzyme solutions have higher cellulase activity as the absorbance decrease values become larger.

    Abstract translation: 一种测定纤维素酶活性的方法，包括：制备两种或多种底物溶液的方法（A），其具有在相同波长下测量的相同吸光度，其中纤维素以相同浓度分散，并测量每种 底物溶液; 分别将不同种类的酶溶液添加到底物溶液中的方法（B），并在相同条件下进行酶反应; 测量酶反应后每种底物溶液的吸光度的方法（C）; 计算酶反应前后底物溶液的吸光度降低值的方法（D）; 以及基于吸光度降低值测定酶溶液的纤维素酶活性的方法（E）。 在方法（E）的测定中，确定随着吸光度降低值变大，酶溶液具有较高的纤维素酶活性。

    METHOD OF REMOVING PROTEIN AGGREGATE
    6.
    发明申请
    METHOD OF REMOVING PROTEIN AGGREGATE 审中-公开

    公开(公告)号：US20180079778A1

    公开(公告)日：2018-03-22

    申请号：US15569424

    申请日：2016-04-28

    Applicant: SHOWA DENKO K.K.

    Inventor： Yuzuru KOKIDO ,  Natsuno MATSUI ,  Hirobumi AOKI ,  Tadashi YONEDA

    IPC: C07K1/18 ,  A61K39/395 ,  C07K16/06

    CPC classification number: C07K1/18 ,  A61K39/39591 ,  C07K16/00 ,  C07K16/065

    Abstract: Provided is a method of removing a protein aggregate which can obtain a useful protein monomer in a high yield and a high purity as a raw material of a pharmaceutical product or the like. The method of removing a protein aggregate includes: a step of making the monomer of a protein and the aggregate of proteins adsorb to the column by making a solution containing a monomer of a protein and an aggregate of proteins pass through a column containing a hard porous polymer self-supporting structure to which a strong cation exchange group is fixed; and a step of making a mobile phase consisting of a mixed solution of a buffer solution and an ionic buffer solution pass through the column to which the monomer of a protein and the aggregate of proteins are adsorbed, to selectively elute the monomer of a protein.

    METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS
    7.
    发明申请
    METHOD FOR TREATING CELLULOSE-CONTAINING BIOMASS 审中-公开
    用于处理含细菌生物质的方法

    公开(公告)号：US20160237616A1

    公开(公告)日：2016-08-18

    申请号：US15027550

    申请日：2014-09-09

    Applicant: SHOWA DENKO K.K.

    Inventor： Ichiro FUJITA ,  Shinji YAMAKI ,  Tadashi YONEDA

    IPC: D21B1/06 ,  B02C19/00 ,  C12P19/14 ,  B30B11/24 ,  C13K1/02 ,  C12P19/02 ,  B02C19/22 ,  B30B15/34

    CPC classification number: D21B1/063 ,  B02C19/0056 ,  B02C19/22 ,  B30B11/24 ,  B30B11/243 ,  B30B15/34 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C13K1/02

    Abstract: A treatment method for biomass, which is a pretreatment method including continuously performing hydrothermal treatment using a screw extruder to produce a biomass composition for saccharification from cellulose-containing biomass serving as a raw material, the treatment method sequentially including: pulverizing the cellulose-containing biomass serving as a raw material so as to have a maximum grain size of 1,000 μm or less and adjusting a water content ratio thereof to from 20% to 80%; performing, in a heating section of the screw extruder, hydrothermal treatment at a temperature of from 150° C. to 250° C. for 0.1 minute to 10 minutes while performing kneading pulverization having a grinding effect; and cooling, in a cooling section of the screw extruder downstream of the heating section, a treated product to 100° C. or less to recover the treated product of the cellulose-containing biomass having an average grain size of 100 μm or less.

    Abstract translation: 一种生物质处理方法，其特征在于，所述预处理方法包括使用螺杆挤出机连续进行水热处理，以生产用作原料的含纤维素生物质的糖化生物质组合物，所述处理方法依次包括：将含纤维素的生物质 作为原料，使其最大粒径为1000μm以下，将其含水率调节为20〜80％。 在螺杆挤出机的加热部分中，在进行具有研磨效果的捏合粉碎的同时，在150℃至250℃的温度下进行0.1分钟至10分钟的水热处理; 在加热部下游的螺杆挤出机的冷却部中，将处理物冷却至100℃以下，回收平均粒径为100μm以下的含纤维素的生物质的处理物。

    METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE
    8.
    发明申请
    METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE 审中-公开
    制备1,4-丁二醇和微生物的方法

    公开(公告)号：US20150353962A1

    公开(公告)日：2015-12-10

    申请号：US14440117

    申请日：2013-09-20

    Applicant: SHOWA DENKO K.K.

    Inventor： Hirobumi AOKI ,  Yuzuru KOKIDO ,  Yoko HASHIMOTO ,  Tadashi YONEDA

    IPC: C12P7/18 ,  C12N9/02

    CPC classification number: C12N9/0008 ,  C12P7/16 ,  C12P7/18 ,  C12Y102/0101 ,  Y02E50/10

    Abstract: A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acyl-CoA reductase, via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA in this order, wherein the reactivity of the acyl-CoA reductase to 4-hydroxybutyryl CoA is greater than or equal to 0.05 times of the reactivity of the acyl-CoA reductase to 3-hydroxybutyryl-CoA.

    Abstract translation: 通过使用酰基辅酶A还原酶的酶反应体系，通过3-羟基丁酰辅酶A，巴豆酰辅酶A和4-羟基丁酰辅酶A按顺序制备1,4-丁二醇的方法，使用微生物和/或其培养物 其中酰基辅酶A还原酶对4-羟基丁酰辅酶A的反应性大于或等于酰基辅酶A还原酶对3-羟基丁酰辅酶A的反应性的0.05倍。

    PROTEIN PURIFICATION METHOD
    10.
    发明申请
    PROTEIN PURIFICATION METHOD 审中-公开

    公开(公告)号：US20180298052A1

    公开(公告)日：2018-10-18

    申请号：US15569466

    申请日：2016-04-28

    Applicant: SHOWA DENKO K.K.

    Inventor： Natsuno MATSUI ,  Yuzuru KOKIDO ,  Hirobumi AOKI ,  Tadashi YONEDA

    IPC: C07K1/18 ,  C07K1/14 ,  C07K1/22

    Abstract: An object of the present invention is to obtain a protein monomer useful as a raw material for medicines industrially and economically in high yield and high purity. In the method for purifying a protein of the present invention, a protein solution containing a protein monomer and a protein aggregate is passed through a column holding a porous rigid polymeric self-supporting structure to which a hydrophobic group is immobilized, and then recovering the protein monomer in a flow-through mode.

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