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    Droplet dispensing systems
    3.
    发明授权
    Droplet dispensing systems 有权

    公开(公告)号：US11919001B2

    公开(公告)日：2024-03-05

    申请号：US16625021

    申请日：2018-06-21

    Applicant: SPHERE FLUIDICS LIMITED

    Inventor： Dimitris Josephides ,  Marian Rehak ,  David Holmes ,  Frank F. Craig

    IPC: B01L3/00

    CPC classification number: B01L3/502784 ,  B01L3/502761 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2400/0487

    Abstract: This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities. We disclose herein a microfluidic droplet dispensing system comprising: a droplet feed line to carry droplets for dispensing; a dispensing line to carry dispensing fluid for dispensing the droplets; a droplet outlet line coupled to a droplet outlet; a first junction having a first input to receive a flow from said droplet feed line, having a second input to receive a flow from said dispensing line and having an output to said droplet outlet line; a waste line coupled to said droplet output line between said first junction and said droplet output; a first pressure source to provide pressurised dispensing fluid to said dispensing line to eject droplets from said droplet output; a droplet dispensing valve between said first pressure source and said dispensing line to control the ejection of droplets from said droplet output; wherein the system further comprises: a second pressure source to provide pressurised dispensing fluid to a droplet protect line; a second junction having a first input from said droplet feed line, having a second input from said droplet protect line, and having an output; and a volume flow throttle coupled between the output of said second junction and the first input of said first junction to restrict a volume flow from said second junction into said first junction.

    SYSTEM AND METHODS
    4.
    发明申请
    SYSTEM AND METHODS 有权

    公开(公告)号：US20210308682A1

    公开(公告)日：2021-10-07

    申请号：US17349068

    申请日：2021-06-16

    Applicant: SPHERE FLUIDICS LIMITED

    Inventor： Frank F. CRAIG ,  Marian Rehak ,  David Holmes ,  Clive A. Smith ,  Xin Liu ,  Giuseppe Benazzi ,  Xin Li ,  Vinayaka Pawate ,  Robert Frank Marchington ,  Richard Francis Day ,  Michael Stewart Hazell

    IPC: B01L3/00 ,  G01N35/08 ,  G01N15/14 ,  C12M1/00

    Abstract: We describe instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one embodiment a method of such processing comprises: providing a plurality of the entities in a fluid: preparing a droplet from said fluid: determining whether said droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of said determination; and dispensing said sorted droplet into a reservoir, wherein said dispensing comprises: identifying said sorted droplet for dispensing; extracting said sorted droplet from a first fluidic flow path of said fluid by transferring said sorted droplet from said first fluidic flow path into a second fluidic flow path; and ejecting said sorted droplet from said second fluidic flow path into said reservoir by applying pressure to said second fluidic flow path. In preferred embodiments the droplet contents are tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and afterwards retrieved for further processing or analysis, or for subsequent use, for example in fermenting or the like.

    DROPLET PROCESSING METHODS AND SYSTEMS
    5.
    发明申请
    DROPLET PROCESSING METHODS AND SYSTEMS 有权

    公开(公告)号：US20210293691A1

    公开(公告)日：2021-09-23

    申请号：US17266447

    申请日：2019-08-06

    Applicant: Sphere Fluidics Limited

    Inventor： Nicholas Dayrell-Armes ,  David Holmes ,  Frank F Craig ,  Marian Rehak ,  Dimitris Josephides ,  Robert Salter ,  William Whitley ,  Sinan Gokkaya ,  Raphael Ruis

    IPC: G01N15/14 ,  B01L3/00 ,  G06K9/00 ,  G06K9/62 ,  G06T7/70

    Abstract: A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.

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