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12 条记录 (耗时 0.017 秒)

    Droplet sorting based on ionization mass spectrometry
    1.
    发明授权
    Droplet sorting based on ionization mass spectrometry 有权

    公开(公告)号：US10545083B2

    公开(公告)日：2020-01-28

    申请号：US15502630

    申请日：2015-08-07

    申请人： Sphere Fluidics Limited

    发明人： Clive Adrian Smith ,  Xin Li ,  Frank F. Craig

    IPC分类号： G01N15/10 ,  B01L3/00 ,  H01J49/04 ,  H01J49/00

    摘要： We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.

    Microfluidic structures
    3.
    发明授权
    Microfluidic structures 有权

    公开(公告)号：US10828640B2

    公开(公告)日：2020-11-10

    申请号：US15754754

    申请日：2016-09-06

    申请人： SPHERE FLUIDICS LIMITED

    发明人： Xin Li ,  Xin Liu ,  Alexandra Clay

    IPC分类号： B01L3/00 ,  B01L3/02

    摘要： A microfluidic structure for spacing out and aligning entities in an aqueous suspension is provided. The structure comprises: a channel for guiding entities in an aqueous suspension; a first comb of first inlets arranged on a first side of the channel for introducing a spacing medium into the channel; and a second comb of second inlets arranged on a second side of the channel for introducing the spacing medium into the channel; wherein the first side is opposite the second side, and wherein one of the first inlets has a corresponding, respective one of the second inlets at a substantially similar longitudinal position along the channel.

    Microfluidic devices and systems
    4.
    发明授权
    Microfluidic devices and systems 有权

    公开(公告)号：US10247655B2

    公开(公告)日：2019-04-02

    申请号：US14907867

    申请日：2014-07-30

    申请人： Sphere Fluidics Limited

    发明人： Clive A. Smith ,  Xin Li ,  Karine Enesa ,  Xin Liu ,  Frank F. Craig ,  Graeme Whyte

    IPC分类号： G01N15/14 ,  B01L3/00 ,  C12Q1/04 ,  C12Q1/06 ,  G01N21/49 ,  G01N15/00

    摘要： A method of label-free cell or particle sorting in a microfluidic device includes providing a stream of aqueous droplets in oil in a channel of the microfluidic device, wherein at least some of the droplets include cells or particles and illuminating the stream from a first direction. Then, one detects scattered light from cells or particles within said aqueous droplets in a second direction. Next, one determines a number of the cells or particles in each droplet from the scattered light and sorts the aqueous droplets into differentiated streams. Notably, the refractive index of said oil is modified to closely match a refractive index of the aqueous droplets to reduce light scattered from boundaries of the droplets. A volume of the droplets can also controlled.

    EMULSION
    5.
    发明申请
    EMULSION 审中-公开
    乳液

    公开(公告)号：US20160169788A1

    公开(公告)日：2016-06-16

    申请号：US14907936

    申请日：2014-07-30

    申请人： SPHERE FLUIDICS LIMITED

    发明人： Clive A. SMITH ,  Xin Li ,  Karing ENESA ,  Xin LIU ,  Frank F. CRAIG ,  Graeme WHYTE

    IPC分类号： G01N15/14

    CPC分类号： G01N15/1484 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  G01N2015/1481 ,  G01N2015/149

    摘要： The present invention provides an emulsion for sorting droplets in a microfluidic device, the emulsion comprising: a discontinuous aqueous phase; and a continuous oil phase; wherein said aqueous phase comprises at least one analyte, said oil phase comprises a fluorous oil and a refractive index modifying compound comprising at least one aromatic ring, and the refractive index of said aqueous phase and the refractive index of said oil phase are substantially matched.

    摘要翻译： 本发明提供了一种用于在微流体装置中分选液滴的乳液，所述乳液包括：不连续的水相; 和连续油相; 其中所述水相包含至少一种分析物，所述油相包括含氟油和包含至少一个芳环的折射率改性化合物，并且所述水相的折射率和所述油相的折射率基本匹配。

    SYSTEM AND METHODS
    10.
    发明申请
    SYSTEM AND METHODS 有权

    公开(公告)号：US20210308682A1

    公开(公告)日：2021-10-07

    申请号：US17349068

    申请日：2021-06-16

    申请人： SPHERE FLUIDICS LIMITED

    发明人： Frank F. CRAIG ,  Marian Rehak ,  David Holmes ,  Clive A. Smith ,  Xin Liu ,  Giuseppe Benazzi ,  Xin Li ,  Vinayaka Pawate ,  Robert Frank Marchington ,  Richard Francis Day ,  Michael Stewart Hazell

    IPC分类号： B01L3/00 ,  G01N35/08 ,  G01N15/14 ,  C12M1/00

    摘要： We describe instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one embodiment a method of such processing comprises: providing a plurality of the entities in a fluid: preparing a droplet from said fluid: determining whether said droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of said determination; and dispensing said sorted droplet into a reservoir, wherein said dispensing comprises: identifying said sorted droplet for dispensing; extracting said sorted droplet from a first fluidic flow path of said fluid by transferring said sorted droplet from said first fluidic flow path into a second fluidic flow path; and ejecting said sorted droplet from said second fluidic flow path into said reservoir by applying pressure to said second fluidic flow path. In preferred embodiments the droplet contents are tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and afterwards retrieved for further processing or analysis, or for subsequent use, for example in fermenting or the like.